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RESEARCH ARTICLE
Contents Vol 55(10)

Pollen fertility in Musa: Viability in cultivars grown in Southern Australia

J. A. Fortescue A B and D. W. Turner A
+ Author Affiliations
- Author Affiliations

A School of Plant Biology (M084), Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

B Corresponding author. email: jafortes@cyllene.uwa.edu.au

Australian Journal of Agricultural Research 55(10) 1085-1091 https://doi.org/10.1071/AR04078
Submitted: 30 March 2004  Accepted: 23 August 2004   Published: 25 October 2004

Abstract

Pollen viability was examined by recording the number of viable as well as non-viable microspores in anthers just before anthesis, as it could be an efficient and rapid indicator of female fertility. It was thought that competency in meiotic restitution in microspores could indicate similar competency in megaspores. Pollen fertility was compared among seeded diploids and edible triploids of Musa and correlated with ovule fertility. Viability was examined using the Alexander’s pollen stain procedure. The seeded diploid species M. acuminata, M. balbisiana, and M. ornata had 3 times more viable pollen than the edible tetraploids (AAAB). M. balbisiana and M. ornata had significantly more viable pollen than M. acuminata. The tetraploids contained 3 times more viable pollen than the edible triploids AAA and ABB and 4 times more than the AAB cultivars. The genome A or B did not affect pollen viability within the triploid cultivars examined. The AAA triploid Gros Michel had the highest percentage of viable pollen at 13% and Green/Red the lowest at 3%. Pollen viability was influenced by meiotic disturbances and an association was made between viability of pollen and viability of embryo sacs.

Pollen viability from anthers of Australian grown species and cultivars of Musa a. ssp. (undescribed subspecies) was compared with published reports of pollen viability from Indian grown species and cultivars. The pollen viability between the Indian cultivated and wild diploids of Musa a. ssp. was similar at 50–66%, but less than the pollen viability of Australian diploid Musa a. ssp of 84%. The Indian-grown triploid cultivars had 21–29% viable pollen and the tetraploids had 28% viable pollen, whereas the Australian grown triploids had 6–10% viable pollen and the tetraploids 29% viable pollen.

There was a difference in pollen viability between genome groups and within genome groups. Different species and cultivars of Musa possess different levels of competency in the production of microspores, which correlated positively with levels of megaspore fertility in the same species and cultivars.

Additional keywords: diploids, Musa acuminata, Musa balbisiana, ovules, pollen viability, tetraploids, triploids.


Acknowledgments

We wish to thank a referee for their insightful comments, staff at the Perth Zoological Gardens, the NSW Agriculture’s Tropical Fruit Research Station, Alstonville, and the plantation at Cudgen, NSW for permission to access their collections of Musa germplasm.


References


Alexander MP (1969) Differential staining of aborted and non aborted pollen. Stain Technology 44, 117–122.
| PubMed |
open url image1

Dahlgren, RMT , Clifford, HT ,  and  Yeo, PF (1985). ‘The families of the monocotyledons. Structure evolution and taxonomy.’ (Springer-Verlag: Berlin)

Dodds KS (1945) Genetical and cytological studies of Musa. VI. The development of female cells of certain edible diploids. Journal of Genetics 46, 161–179. open url image1

Dodds KS, Simmonds NW (1948) Sterility and parthenocarpy in diploid hybrids of Musa. Heredity 2, 101–107. open url image1

Fortescue JA (2002) Reproductive biology of the Musaceae: ovule ontogeny of bananas, plantains and Ensat. Unpublished thesis, The University of Western Australia.

Johri, BM , Ambegaokae, KB ,  and  Srivastava, PS (1992). ‘Comparative embryology of angiosperms.’ Vol. 1–2, (Springer-Verlag: Berlin)

O’Brien, TP ,  and  McCully, ME (1981). ‘The study of plant structure: principles and selected methods.’ (Termarcarphi: Australia)

Sathiamoorthy S (1994) Musa improvement in India. ‘The improvement and testing of : a global partnership’. (Ed. DR Jones) pp. 188–200. (INIBAP: Montpellier, France)

Sathiamoorthy S, Madhava Rao VN (1980) Pollen production in relation to genome and ploidy in banana clones. ‘National Seminar on Banana Production Technology’. (Ed.  CR Muthukrishnan , JBMMd Abdul Khader ) pp. 46–49. (Tamil Nadu Agricultural University: Chennai, TN)


Shepherd K (1960) Seed fertility of edible bananas. Journal of Horticultural Science 35, 6–20. open url image1

Shepherd, K (1999). ‘Cytogenetics of the genus .’ (INIBAP: Montpellier, France)

Simmonds, NW (1962). ‘The evolution of the bananas.’ (Longman: London)

Stover, RH ,  and  Simmonds, NW (1987). ‘Bananas.’ 3rd edn . (Longman: London)