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Utilization of oocytes from closely related domestic species for interspecies somatic cell nuclear transfer (iSCNT) in gaur embryo production

Ratree Jintana, Kriengsak Tasripoo, Kitiya Srisakwattana , Wanvipa Suthikrai, Sunpetch Sophon, Theerawat Tharasanit

Abstract

Context: The limited availability of recipient oocytes poses significant challenges in increasing the population of endangered species. Aim: This study demonstrates the use of oocytes from closely related domestic species (bovine, buffalo, and goat) in the production of gaur embryos via interspecies somatic cell nuclear transfer (iSCNT). The developmental abilities of embryos from various combinations—gaur-bovine, gaur-buffalo, gaur-goat, and others—are analyzed. Previous achievements are retrospectively reviewed and summarized. Methods: The methodologies for iSCNT and in vitro gaur embryo production are outlined. Key results: Blastocyst rates among gaur-bovine, gaur-buffalo, and gaur-goat embryos showed no significant differences (P<0.05). Gaur-bovine embryo development was comparable to intraspecies embryos (bovine-bovine), indicating successful developmental compatibility. Pregnancy rates after transferring gaur-bovine embryos were first reported, with rates of 4.76%, 42.86%, 19.05%, and 4.76% at Days 45, 60, 90, and 120, respectively. Conclusion: Bovine, buffalo, and goat oocyte cytoplasm is effective for producing gaur iSCNT embryos, capable of initiating early pregnancies. Implications: Laboratory techniques and methodologies can save costs and time, contributing to the conservation of endangered species.

AN24218  Accepted 17 December 2024

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