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RESEARCH ARTICLE
Contents Vol 49(12)

A bromochloromethane formulation reduces enteric methanogenesis in cattle fed grain-based diets

N. W. Tomkins A C , S. M. Colegate B and R. A. Hunter A
+ Author Affiliations
- Author Affiliations

A CSIRO Livestock Industries, JM Rendel Laboratory, PO Box 5545, CQ Mail Centre, Qld 4702, Australia.

B CSIRO Livestock Industries, Plant Toxins Unit, Australian Animal Health Laboratory, Portarlington Road, East Geelong, Vic. 3219, Australia.

C Corresponding author. Email: nigel.tomkins@csiro.au

Animal Production Science 49(12) 1053-1058 https://doi.org/10.1071/EA08223
Submitted: 29 August 2008  Accepted: 15 July 2009   Published: 16 November 2009

Abstract

Enteric fermentation has been estimated to be responsible for 64.2 Mt CO2-e, or ~16% of Australia’s greenhouse gas emissions (Australian Greenhouse Office 2007). A bromochloromethane (BCM) formulation, previously shown to inhibit methanogenesis, was included in the diet of Brahman (Bos indicus) cross steers, twice daily in three separate experiments, to determine the effect on methane production, daily feed intake, liveweight (LW) gain and accumulation of residues of BCM in edible tissue.

In the first experiment, the BCM formulation was fed at rates of 0, 0.15, 0.30, or 0.60 g/100 kg LW, twice daily, for 28 days. Methane production (mean ± s.e.), measured over 11 h after feed was first consumed on day 28, was 0.3 ± 0.13 and 0.1 ± 0.03 L/h for animals treated at a rate of 0.30 and 0.60 g/100 kg LW, respectively. This was significantly less (P < 0.05) than for control animals (4.6 ± 0.46 L/h) and animals treated at a rate of 0.15 g/100 kg LW (2.1 ± 0.28 L/h). The dose rate of 0.30 g/100 kg LW was associated with a decrease in methanogenesis by ~93% compared with the control group and was used in subsequent experiments.

The second experiment evaluated the efficacy of the BCM formulation fed at rates of 0 or 0.30 g/100 kg LW, twice daily, for 90 days. Methane production was measured over 24-h periods, on days 30, 60 and 90. For days 30 and 90, methane production was reduced by 60% (P < 0.05) to 4.2 ± 1.82 L/h and by 50% (P < 0.05) to 6.1 ± 0.63 L/h, respectively, for treated animals compared with the control group.

The final experiment determined the effect on LW gain and detectable residues in edible tissue, with animals given the BCM formulation at rates of 0 or 0.30 g/100 kg LW, twice daily, for 85 days. Liver, kidney, depot fat and muscle samples collected 1 and 10 days after the last day of treatment had concentrations of BCM that did not exceed 0.015 mg/kg and were less than the temporary maximum residue limit (0.02 mg/kg BCM), which applies to bovine meat, fat and edible offal. There were no significant differences in LW gain (1.4 ± 0.10 v. 1.5 ± 0.07 kg/day), feed conversion ratio (5.7 ± 0.32 v. 5.4 ± 0.09), hot carcass weight (235 ± 5.0 v. 250 ± 6.5 kg) or P8 fat depth (6.4 ± 0.89 v. 8.1 ± 1.15 mm) between control and treated animals.

The experiments reported here were completed in 2004 before the Australian Government prohibited the manufacture and use of BCM. It is unlikely that the BCM formulation will be available for commercial use to mitigate livestock methane emissions in Australia. Nevertheless, the study has demonstrated that methane emissions were substantially reduced over a 90-day feedlot finishing period. This indicates that alternative antimethanogens with a similar mechanism of action may have practical commercial relevance.

Additional keywords: antimethanogen, cyclodextrin, methane, residues.


Acknowledgements

The authors acknowledge the valuable contribution of Neil Anderton, Plant Toxins Research Group, (CSIRO Livestock Industries, Geelong) for preparing the BCM formulation; the valuable support and interest of Marion Andrew (CSIRO Livestock Industries, Geelong); Karina Tane, Chris O’Neill, Alan Day and Bill van den Heuvel for assisting in monitoring respiration chambers; Dean Gibson for training and care of experimental animals (CSIRO Livestock Industries, Rockhampton); Robert Dickinson (Food Science Australia, Canon Hill), and AQIS and abattoir staff (AMH Nerimba, Queensland) for slaughter and tissue collection. Peter Kennedy (CSIRO Livestock Industries, Rockhampton) is acknowledged for his valuable and constructive comments on the manuscript.


References


Anderson RC, Carstens GE, Miller RK, Callaway TR, Schultz CL, Edrington TS, Harvey RB, Nisbet DJ (2004) Effect of nitro ethane administration on ruminal VFA production and specific activity of methane production. Journal of Animal and Feed Sciences 13, 23–26. open url image1

Australian Greenhouse Office (2007) ‘National greenhouse gas inventory 2005.’ (Commonwealth of Australia: Canberra)

Bruce HL, Stark JL, Beilken SL (2004) The effects of finishing diet and post-mortem ageing on the eating quality of the M. longissimus thoracis of electrically stimulated Brahman steer carcasses. Meat Science 67, 261–268.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Chalupa W (1977) Manipulating rumen fermentation. Journal of Animal Science 46, 585–599. open url image1

Coleman P , Buddhadasa S , Santhakumar M , Paranandi B (2003) Evaluation of a novel antimethanogen in feedlot cattle. Study report VGLP08/R1. Australian Government Analytical Laboratories, Melbourne.

Denman SE , Tomkins NW , McSweeeney CS (2005) Monitoring the effect of bromochloromethane on methanogen populations within the rumen using qPCR. In ‘2nd international conference on greenhouse gases and animal agriculture GGAA2005’. (Eds CR Soliva, J Takahashi, M Kreuzer) pp. 112–114. (Eidgenössische Technische Hochschule: Zürich)

Denman SE, Tomkins NW, McSweeney CS (2007) Quantitation and diversity analysis of ruminal methanogenic populations in response to the antimethanogenic compound Bromochloromethane. FEMS Microbiology Ecology 62, 313–322.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Hindrichsen IK, Wettstein H-R, Machmüller A, Soliva CR, Bach Knudsen KE, Madsen J, Kreuzer M (2004) Effects of feed carbohydrates with contrasting properties on rumen fermentation and methane release in vitro. Canadian Journal of Animal Science 84, 265–276. open url image1

IPCC (1992) Intergovernmental panel on climate change. Climate Change 1992. The supplementary report to the IPCC Scientific Assessment, Cambridge University Press, Cambridge.

Johnson KA, Johnson DE (1995) Methane emissions from cattle. Journal of Animal Science 73, 2483–2492.
|  CAS | PubMed |
open url image1

Johnson DE, Ward GM (1996) Estimates of animal methane emissions. Environmental Monitoring and Assessment 42, 133–141.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Johnson DE, Wood AS, Stone JB, Moran ET (1972) Some effects of methane inhibition in ruminants (steers). Canadian Journal of Animal Science 52, 703–712.
|  CAS | Crossref |
open url image1

Loftsson T, Brewster ME (1996) Pharmaceutical applications of cyclodextrins. 1. Drug solubilization and stabilization. Journal of Pharmaceutical Sciences 85, 1017–1025.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Machmüller A, Soliva CR, Kreuzer M (2003) Methane-suppressing effect of myristic acid in sheep as affected by dietary calcium and forage proportion. The British Journal of Nutrition 90, 529–540.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

May C , Payne A , Stewart P , Edgar J (1995) A delivery system for agents. International patent application no. PCT/AU95/00733.

McAllister TA, Okine EK, Mathison GW, Cheng KJ (1996) Dietary, environmental and microbial aspects of methane production in ruminants. Canadian Journal of Animal Science 76, 231–243.
|  CAS | Crossref |
open url image1

McCrabb GJ, Hunter RA (1999) Prediction of methane emissions from beef cattle in tropical production systems. Australian Journal of Agricultural Research 50, 1335–1339.
| Crossref | GoogleScholarGoogle Scholar | open url image1

McCrabb GJ, Berger KT, Magner T, May C, Hunter RA (1997) Inhibiting methane production in Brahman cattle by dietary supplementation with a novel compound and the effects on growth. Australian Journal of Agricultural Research 48, 323–329.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Mohammed N, Lila ZA, Ajisaka K, Hara K, Mikuni K, Hara K, Kanda S, Itabashi H (2004a) Inhibition of ruminal microbial methane production by β-cyclodextrin iodopropane, malate and their combination in vitro. Journal of Animal Physiology and Animal Nutrition 88, 188–195.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Mohammed N, Ajisaka N, Lila Z, Hara K, Mikuni K, Hara K, Kanda S, Itabashi H (2004b) Effect of Japanese horseradish oil on methane production and ruminal fermentation in vitro and in steers. Journal of Animal Science 82, 1839–1846.
|  CAS | PubMed |
open url image1

NRA (2002) National Registration Authority. Amendment No. 6F. Amendments to the MRL standard. National Registration Authority. NRA Gazette no. 12, 3 December.

Sawyer MS, Hoover WH, Sniffen CJ (1974) Effects of a ruminal methane inhibitor on growth and energy metabolism in the ovine. Journal of Animal Science 38, 908–914.
|  CAS | PubMed |
open url image1

Svirbely JL, Highman B, Alford WC, von Oettingen WF (1947) The toxicity and narcotic action of mono chloro mono Bromo-methane with special reference to inorganic and volatile bromide in blood, urine and brain. The Journal of Industrial Hygiene and Toxicology 29, 382–389.
|  CAS |
open url image1

Turner HG, Thornton RF (1966) A respiration chamber for cattle. Proceedings of the Australian Society of Animal Production 6, 413–419. open url image1

Wood JM, Kennedy FS, Wolfe RS (1968) The reaction of multihalogenated hydrocarbons with free and bound reduced vitamin B12. Biochemistry 7, 1707–1713.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1