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RESEARCH ARTICLE
Contents Vol 64(1)

Replacing bacitracin zinc antibiotic with symbiotic additive in pullet diet

A. G. Ribeiro https://orcid.org/0000-0001-6730-0209 A * , C. B. V. Rabello https://orcid.org/0000-0002-5912-162X A , M. J. B. Santos https://orcid.org/0000-0002-6023-3426 A , J. C. S. Nascimento https://orcid.org/0000-0003-3107-5876 A , D. A. Silva https://orcid.org/0000-0001-6243-3969 A , E. S. R. Soares https://orcid.org/0000-0001-8271-8056 A , H. E. C. C. C. Manso https://orcid.org/0000-0002-4128-3831 A , W. R. L. Medeiros-Ventura https://orcid.org/0000-0001-5796-2321 A , R. V. Silva Junior https://orcid.org/0000-0002-0208-1065 A , M. A. Siqueira https://orcid.org/0000-0002-0367-855X A and S. P. R. Silva https://orcid.org/0000-0002-2235-3507 B
+ Author Affiliations
- Author Affiliations

A Universidade Federal Rural de Pernambuco, Animal Science Department, Recife, Brazil.

B Universidade de Pernambuco, Mechanical Engineering Department, Recife, Brazil.

* Correspondence to: apoloniogomes962@gmail.com

Handling Editor: Natalie Morgan

Animal Production Science 64, AN23299 https://doi.org/10.1071/AN23299
Submitted: 12 April 2023  Accepted: 5 October 2023  Published: 26 October 2023

© 2024 The Author(s) (or their employer(s)). Published by CSIRO Publishing

Abstract

Context

Currently, since the use of antibiotics as growth promoters in animals has been banned worldwide, it is necessary to use other additives that indorse an adequate balance of the intestinal microbiota, so as to render better results in nutrient acquisition and health of poultry, yielding adequate growth and performance.

Aim

The study evaluated the effects of dietary supplementation with symbiotics during the grower phase, to replace bacitracin zinc.

Methods

The experiment included a total of 684 pullets of the Dekalb White strain, from 6 to 15 weeks of age, distributed in a completely randomised design, with five treatments, and eight replications of 18 birds each. The treatments were composed of two base diets, namely one of corn and soybean meal (reference diet, RD), and another of meat and bone meal (MBM), in addition to two more diets based on MBM, one with an addition of 0.05% of bacitracin zinc (Bac Zn), and the other with 0.1% of symbiotic provided to two groups, including one group that had already consumed the symbiotic since the first day of life (starter phase, Symb-S), and the other that started to consume at the beginning of the experiment (grower phase, Symb-G). Performance, blood variables, nutrient metabolisability, weights of organs of the immune and digestive system, and measurement of length (intestine and caecum) were determined. Data were compared using orthogonal contrast.

Key results

The birds fed with the RD diet presented a worse overall performance and coefficients of apparent metabolisability of dry matter (CAMDM) (6–10 weeks) than did those fed the MBM diet. Birds fed with the Bac Zn diet showed better bodyweight gain (6–15 weeks), coefficients of apparent metabolisability of gross energy (CAMGE) and CAMDM (6–10 weeks) than did those fed the MBM diet. Birds fed with symbiotic diets had better responses in blood biochemistry variables (alkaline phosphatase, total proteins) than did those fed the Bac Zn diet.

Conclusion

The use of symbiotics since the starter and grower phase can replace the use of Bac Zn, without affecting the biochemical and metabolic responses of the pullets.

Implications

Symbiotics improved utilisation of diet nutrients, maintenance of physiological homeostasis, and growth of pullets.

Keywords: blood variables, growth promoters, gut microbiota, nutrient metabolisability, performance, prebiotics, probiotics, supplementation.

References

Abdel-Wareth AAA, Hammad S, Khalaphallah R, Salem WM, Lohakare J (2019) Synbiotic as eco-friendly feed additive in diets of chickens under hot climatic conditions. Poultry Science 98(10), 4575-4583.
| Crossref | Google Scholar | PubMed |

Al-Khalaifa H, Al-Nasser A, Al-Surayee T, Al-Kandari S, Al-Enzi N, Al-Sharrah T, Ragheb G, Al-Qalaf S, Mohammed A (2019) Effect of dietary probiotics and prebiotics on the performance of broiler chickens. Poultry Science 98, 4465-4479.
| Crossref | Google Scholar | PubMed |

Al-Khalaifah HS (2018) Benefits of probiotics and/or prebiotics for antibiotic-reduced poultry. Poultry Science 97, 3807-3815.
| Crossref | Google Scholar | PubMed |

Alavi SAN, Zakeri A, Kamrani B, Pourakbari Y (2012) Effect of prebiotics, probiotics, acidfire, growth promoter antibiotics and synbiotic on humural immunity of broiler chickens. Global Veterinaria 8, 612-617.
| Google Scholar |

AOAC (2003) ‘Official methods and recommended practices of the American Oil Chemists Society.’ (AOCS: Champaign, IL, USA)

Beirão BCB (2011) Avaliação Do Perfil Imune De Aves Empregando Citometria de Fluxo. Dissertação (MSc), Universidade Federal do Paraná, Curitiba, Brazil. Available at http://hdl.handle.net/1884/25691

Bilal M, Si W, Barbe F, Chevaux E, Sienkiewicz O, Zhao X (2021) Effects of novel probiotic strains of Bacillus pumilus and Bacillus subtilis on production, gut health, and immunity of broiler chickens raised under suboptimal conditions. Poultry Science 100(3), 100871.
| Crossref | Google Scholar | PubMed |

Bozkurt M, Alçiçek A, Çabuk M (2004) The effect of dietary inclusion of meat and bone meal on the performance of laying hens at old age. South African Journal of Animal Sciences 34, 31-36.
| Crossref | Google Scholar |

BRASIL (2020) Proibição em território nacional de aditivos melhoradores de desempenho que contenham antimicrobianos classificados como importantes na medicina humana. Instrução Normativa n° 1, de 13 de janeiro de. Diário Oficial da União, Ministério da Agricultura, Pecuária e Abastecimento/Secretaria de Defesa Agropecuária, Brasília, DF, 23 January 2020. Seção 1, ed. 16, p. 6.

Cardozo MV (2011) Salmonella spp. e Clostridium perfringens em farinhas de origem animal utilizadas na fabricação de rações e avaliação de aditivo na inibição de patógeno. Dissertação (MSc), Universidade Federal Paulista ‘Julio de Mesquita Filho’, Jaboticabal, Brazil. Available at http://hdl.handle.net/11449/94934

Casagrande MF (2012) Clostridium perfringens em ingredientes para ração de aves e controle da presença do agente utilizando tratamento químico. Dissertação (MSc), Universidade Federal Paulista ‘Julio de Mesquita Filho’, Jaboticabal, Brazil. Available at http://hdl.handle.net/11449/94884

Castanon JIR (2007) History of the use of antibiotic as growth promoters in european poultry feeds. Poultry Science 86, 2466-2471.
| Crossref | Google Scholar | PubMed |

Costa T, Linhares I, Ferreira R, Neves J, Almeida A (2018) Frequency and antibiotic resistance of bacteria implicated in community urinary tract infections in North Aveiro between 2011 and 2014. Microbial Drug Resistance 24, 493-504.
| Crossref | Google Scholar | PubMed |

Dekalb (2009) Dekalb White Commercial Management Guide. Available at https://www.dekalbpoultry.com/documents/1827/Dekalb_White_CS_management_guide__North_American_Version_L2221-1.pdf

Deng Q, Shi H, Luo Y, Zhao H, Liu N (2020) Effect of dietary Lactobacilli mixture on Listeria monocytogenes infection and virulence property in broilers. Poultry Science 99, 3655-3662.
| Crossref | Google Scholar | PubMed |

Duncan SH, Louis P, Flint HJ (2004) Lactate-utilizing bacteria, isolated from human feces, that produce butyrate as a major fermentation product. Applied and Environmental Microbiology 70, 5810-5817.
| Crossref | Google Scholar | PubMed |

Fathima S, Shanmugasundaram R, Adams D, Selvara RK (2022) Gastrointestinal microbiota and their manipulation for improved growth and performance in chickens. Foods 11, 1401.
| Crossref | Google Scholar | PubMed |

FDA (2018) Summary report on antimicrobials sold or distributed for use in food-producing animals. Center for Veterinary Medicine 7, 1–25. Available at https://www.fda.gov/media/133411/download#:~:text=Of%20the%202018%20domestic%20sales,fluoroquinolones%20for%20less%20than%201

Feitosa TJO, Silva CE, Souza RG, Lima CDS, Gurgel AC, Oliveira LLG, Nóbrega JGS, Júnior JEMC, Melo FO, Santos WBM, Feitoza TO, Costa TF, Brandão PA, Minafra CS (2020) Microbiota intestinal das aves de produção: revisão bibliográfica. Research, Society and Development 9, e42952779.
| Crossref | Google Scholar |

Forte C, Manuali E, Abbate Y, Papa P, Vieceli L, Tentellini M, Trabalza-Marinucci M, Moscati L (2018) Dietary Lactobacillus acidophilus positively influences growth performance, gut morphology, and gut microbiology in rurally reared chickens. Poultry Science 97, 930-936.
| Crossref | Google Scholar | PubMed |

Gadde UD, Oh S, Lillehoj HS, Lillehoj EP (2018) Antibiotic growth promoters virginiamycin and bacitracin methylene disalicylate alter the chicken intestinal metabolome. Scientific Reports 8, 3592.
| Crossref | Google Scholar | PubMed |

Gibson GR, Roberfroid MB (1995) Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. The Journal of Nutrition 125, 1401-1412.
| Crossref | Google Scholar | PubMed |

Harwood CR, Mouillon JM, Pohl S, Arnau J (2018) Secondary metabolite production and the safety of industrially important members of the Bacillus subtilis group. FEMS Microbiology Reviews 42, 721-738.
| Crossref | Google Scholar | PubMed |

Haseeb M, Lakho SA, Huang J, Hasan MW, Naqvi MA, Zhou Z, Yan R, Xu L, Song X, Li X (2020) In vitro effects of 5 recombinant antigens of Eimeria maxima on maturation, differentiation, and immunogenic functions of dendritic cells derived from chicken spleen. Poultry Science 99, 5331-5343.
| Crossref | Google Scholar | PubMed |

Kalani M, Rahimi SH, Salehi TZ, Hajiaghaee R, Behnamifar A (2022) Comparison the effects of probiotic and prebiotic as antibiotic alternatives on Salmonella colonization, performance, and egg quality in laying hens challenged with Salmonella enterica serotype Enteritidis. Iranian Journal of Veterinary Research 23(2), 154-162.
| Crossref | Google Scholar | PubMed |

Khan S, Moore RJ, Stanley D, Chousalkar KK (2020) The gut microbiota of laying hens and its manipulation with prebiotics and probiotics to enhance gut health and food safety. Applied and Environmental Microbiology 86, e00600-20.
| Crossref | Google Scholar |

Kuritza LN, Westphal P, Santin E (2014) Probióticos na avicultura. Ciencia Rural 44, 1457-1465.
| Crossref | Google Scholar |

Lemos MJ, Calixto LFL, Torres-Cordido KAA, Reis TL (2016) Uso de aditivo alimentar equilibrador da flora intestinal em aves de corte e de postura. Arquivos do Instituto Biológico 83, e0862014.
| Crossref | Google Scholar |

Matur E, Ergul E, Akyazi I, Eraslan E, Cirakli ZT (2010) The effects of Saccharomyces cerevisiae extract on the weight of some organs, liver, and pancreatic digestive enzyme activity in breeder hens fed diets contaminated with aflatoxins. Poultry Science 89, 2213-2220.
| Crossref | Google Scholar | PubMed |

Meimandipour A, Shuhaimi M, Soleimani AF, Azhar K, Hair-Bejo M, Kabeir BM, Javanmard A, Anas OM, Yazid AM (2010) Selected microbial groups and short-chain fatty acids profile in a simulated chicken cecum supplemented with two strains of Lactobacillus. Poultry Science 89, 470-476.
| Crossref | Google Scholar | PubMed |

Mendonça AJ (2007) Avaliação hematológica, bioquímica e hemostática de bezerros Brahman provenientes de produção in vitro (PIV) e bezerros Brahman de produção in vivo. Thesis (DSc), Universidade Estadual Paulista, Botucatu, Brazil. Available at http://hdl.handle.net/11449/101311

Mohammed AA, Jiang S, Jacobs JA, Cheng HW (2019) Effect of a synbiotic supplement on cecal microbial ecology, antioxidant status, and immune response of broiler chickens reared under heat stress. Poultry Science 98, 4408-4415.
| Crossref | Google Scholar | PubMed |

Muhammad J, Khan S, Su JQ, Hesham AEL, Ditta A, Nawab J, Ali A (2019) Antibiotics in poultry manure and their associated health issues: a systematic review. Journal of Soils and Sediments 20, 486-497.
| Crossref | Google Scholar |

Pourabedin M, Xu Z, Baurhoo B, Chevaux E, Zhao X (2014) Effects of mannan oligosaccharide and virginiamycin on the cecal microbial community and intestinal morphology of chickens raised under suboptimal conditions. Canadian Journal of Microbiology 60, 255-266.
| Crossref | Google Scholar | PubMed |

Prado OF (2018) Aspectos metabólicos plasmáticos de novilhas nelore e cruzadas confinadas. Dissertação (MSc), Instituto Federal de Educação, Ciências e Tecnologia Goiano, Rio Verde, Brazil.

Rahman MM, Khan MMH, Howlader MMR (2021) Effects of supplementation of probiotics instead of antibiotics to broiler diet on growth performance, nutrient retention, and cecal microbiology. Journal of Advanced Veterinary and Animal Research 8(4), 534-539.
| Crossref | Google Scholar |

Reis MP (2011) Uso da bacitracina de zinco e do sulfato de colistina como melhoradores de desempenho de frangos de corte. Dissertação (MSc),Universidade Federal de Lavras, Minas Gerais, Brazil. Available at http://repositorio.ufla.br/jspui/handle/1/3440

Reis TL, Vieites FM (2019) Antibiótico, Prebiótico, Probiótico E Simbiótico Em Rações De Frangos De Corte E Galinhas Poedeiras. Ciência Animal 29, 133-147 Available at https://revistas.uece.br/index.php/cienciaanimal/article/view/10086.
| Google Scholar |

Ribeiro AG, Rabello CBV, Santos MJB, Nascimento JCS, Silva DA, Oliveira HSH, Macambira GM, Santos ACF, Maciel MS, Carvalho CBM, Mota MAA, Pessoa DV, Silva GD, Siqueira MA, Souza DMMP, Marinho JBM, Silva LAL, Miranda VMMC (2021) Hábitats da microbiota residente e aditivos equilibradores intestinais para aves de produção: revisão. Research, Society and Development 10, e242101018800.
| Crossref | Google Scholar |

Rosolem MC (2017) Relação Entre As Células Dendríticas E Os Linfócitos T Regulatórios Em Neoplasias Mamárias Caninas. Thesis (DSc), Universidade Estadual Paulista, Jaboticabal, Brazil. Available at http://hdl.handle.net/11449/152292

Rostagno HS, Albino LFT, Hannas MI, Donzele JL, Sakomura NK, Perazzo FG, Saraiva A, Teixeira ML, Rodrigues PB, Oliveira RF, Barreto SLT, Brito CO (2017) ‘Tabelas Brasileiras para aves e suínos.’ 4a Edição. (Ed. HS Rostagno) pp. 1–488. (UFV (Viçosa-MG), Departamento de Zootecnia). Available at https://edisciplinas.usp.br/pluginfile.php/4532766/mod_resource/content/1/Rostagno%20et%20al%202017.pdf

Saad SMI (2006) Probióticos e prebióticos: o estado da arte. Revista Brasileira de Ciências Farmacêuticas 42, 1-16.
| Crossref | Google Scholar |

Sakomura NK, Rostagno HS (2007) Planejamento dos experimentos com monogástricos: correção da conversão alimentar pela mortalidade. In ‘Métodos de pesquisa em nutrição de monogástricos. 1’. pp. 30–37. (Funep: Jaboticabal, Brazil)

Sandvang D, Skjoet-Rasmussen L, Cantor MD, Mathis GF, Lumpkins BS, Blanch A (2021) Effects of feed supplementation with 3 different probiotic Bacillus strains and their combination on the performance of broiler chickens challenged with Clostridium perfringens. Poultry Science 100(4), 100982.
| Crossref | Google Scholar | PubMed |

Siewert G, Strominger JL (1967) Bacitracin: an inhibitor of the dephosphorylation of lipid pyrophosphate, an intermediate in the biosynthesis of the peptidoglycan of bacterial cell walls. Proceedings of the National Academy of Sciences of the United States of America 57, 767-773.
| Crossref | Google Scholar |

Silva MN (2017) Hematologia veterinária. Belém: EditAedi, E-book, 110 p. Available at https://livroaberto.ufpa.br/jspui/handle/prefix/734

Śliżewska K, Markowiak-Kopeć P, Żbikowski A, Szeleszczuk P (2020) The effect of synbiotic preparations on the intestinal microbiota and her metabolism in broiler chickens. Scientific Reports 10, 4281.
| Crossref | Google Scholar |

Sokale AO, Menconi A, Mathis GF, Lumpkins B, Sims MD, Whelan RA, Doranalli K (2019) Effect of Bacillus subtilis DSM 32315 on the intestinal structural integrity and growth performance of broiler chickens under necrotic enteritis challenge. Poultry Science 98, 5392-5400.
| Crossref | Google Scholar | PubMed |

Swain BK, Naik PK, Chakurkar EB, Singh NP (2011) Effect of probiotic and yeast supplementation on performance, egg quality characteristics and economics of production in Vanaraja layers. Indian Journal of Poultry Science 46, 313-315.
| Google Scholar |

Sweeney MT, Lubbers BV, Schwarz S, Watts JL (2018) Applying definitions for multidrug resistance, extensive drug resistance and pandrug resistance to clinically significant livestock and companion animal bacterial pathogens. Journal of Antimicrobial Chemotherapy 73, 1460-1463.
| Crossref | Google Scholar | PubMed |

Tang SGH, Sieo CC, Ramasamy K, Saad WZ, Wong HK, Ho YW (2017) Performance, biochemical and haematological responses, and relative organ weights of laying hens fed diets supplemented with prebiotic, probiotic and synbiotic. BMC Veterinary Research 13, 248.
| Crossref | Google Scholar | PubMed |

Van Keulen J, Young BA (1977) Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies. Journal of Animal Science 44, 282-287.
| Crossref | Google Scholar |

Wales AD, Allen VM, Davies RH (2010) Chemical treatment of animal feed and water for the control of Salmonella. Foodborne Pathogens and Disease 7, 3-15.
| Crossref | Google Scholar | PubMed |

WHO (1997) ‘The medical impact of antimicrobial use in food animals.’ (World Health Organization). Available at https://iris.who.int/bitstream/handle/10665/64439/WHO_EMC_ZOO_97.4.pdf

Xiang Q, Wang C, Zhang H, Lai W, Wei H, Peng J (2019) Effects of different probiotics on laying performance, egg quality, oxidative status, and gut health in laying hens. Animals 9(12), 1110.
| Crossref | Google Scholar | PubMed |

Xu H, Lu Y, Li D, Yan C, Jiang Y, Hu Z, Zhang Z, Du R, Zhao X, Zhang Y, Tian Y, Zhu Q, Liu Y, Wang Y (2023) Probiotic mediated intestinal microbiota and improved performance, egg quality and ovarian immune function of laying hens at different laying stage. Frontiers in Microbiology 14(1), 1041072.
| Crossref | Google Scholar |

Youssef IM, Khalil HA, Jaber FA, Alhazzaa RA, Alkholy SO, Almehmadi AM, Alhassani WE, Al-Shehri M, Hassan H, Hassan MS, Halim HSAE, El-Hack MEA, Youssef KM, Abo-Samra MA (2023) Influence of dietary mannan-oligosaccharides supplementation on hematological characteristics, blood biochemical parameters, immune response and histological state of laying hens. Poultry Science 102(11), 103071.
| Crossref | Google Scholar | PubMed |

Zhen W, Zhu T, Wang P, Guo F, Zhang K, Zhang T, Jalukar S, Zhang Y, Bai D, Zhang C, Guo Y, Wang Z, Ma Y (2023) Effect of dietary Saccharomyces-derived prebiotic refined functional carbohydrates as antibiotic alternative on growth performance and intestinal health of broiler chickens reared in a commercial farm. Poultry Science 102(6), 102671.
| Crossref | Google Scholar | PubMed |