Twenty-five new polymorphic microsatellites for the eastern mosquitofish, Gambusia holbrooki (Actinopterygii : Poeciliidae), an invasive species in Australia
Kate D. L. Umbers A C , Michael D. Jennions A , Michael G. Gardner B and J. Scott Keogh AA Research School of Biology, Australian National University, Canberra, ACT 0200, Australia.
B School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia; Australian Centre for Evolutionary Biology and Biodiversity, University of Adelaide, Adelaide, SA 5005, Australia; and Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, SA 5001, Australia.
C Corresponding author. Email: kate.umbers@anu.edu.au
Australian Journal of Zoology 60(4) 235-237 https://doi.org/10.1071/ZO12095
Submitted: 13 September 2012 Accepted: 16 October 2012 Published: 12 November 2012
Abstract
We isolated 25 new polymorphic microsatellite markers from the eastern mosquitofish, Gambusia holbrooki. Initially, 454 shotgun sequencing was used to identify 1187 loci for which primers could be designed. Of these 1187, we trialled 48 in the target species, 40 of which amplified a product of expected size. Subsequently, those 40 loci were screened for variation in 48 individuals from a single population in Canberra, Australia. Twenty loci were in Hardy–Weinberg equilibrium and polymorphic, with observed heterozygosity ranging from 0.04 to 0.72 (mean: 0.45 ± 0.18) and the number of alleles per locus ranged from 2 to 5 (mean: 3.20 ± 1.05). These loci will be useful in understanding genetic variation, paternity analysis and in managing this species across both its native and invasive range.
Additional keywords: 454 GS-FLX, shotgun sequencing.
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