Free Standard AU & NZ Shipping For All Book Orders Over $80!
Register      Login
Sexual Health Sexual Health Society
Publishing on sexual health from the widest perspective
RESEARCH ARTICLE

Detection of the sexually transmissible genital mycoplasmas by polymerase chain reaction in women

Vessela V. Ouzounova-Raykova A B , Rumyana Markovska A , Gergana Mizgova A and Ivan G. Mitov A
+ Author Affiliations
- Author Affiliations

A Department of Medical Microbiology, Medical University of Sofia, ‘Zdrave’2 street 1431, Sofia, Bulgaria.

B Corresponding author. Email: pumpi@abv.bg

Sexual Health 8(3) 445-446 https://doi.org/10.1071/SH11044
Submitted: 7 March 2011  Accepted: 28 March 2011   Published: 17 August 2011

Abstract

Background: The role of Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium in the development of clinical disorders is still unclear. The aim of this study was to determine their prevalence in symptomatic and asymptomatic women. Methods: A total of 348 women were examined by applying polymerase chain reaction (PCR) methods. Results: The registered prevalence was as follows: U. urealyticum 14.66%; M. hominis 3.16%; and M. genitalium 0.29%. Co-infection was established in 11 swabs. Conclusions: This is the first study in Bulgaria for the detection of mycoplasmas by PCR. Our results demonstrate similar or lower values in comparison with other researchers and further investigations are needed.

Additional keywords: Bulgaria, Mycoplasma hominis, Mycoplasma genitalium, prevalence, Ureaplasma urealyticum.


References

[1]  Lee SR, Chung JM, Kim YG. Rapid one step detection of pathogenic bacteria in urine with sexually transmitted disease (STD) and prostatitis patient by multiplex PCR assay (mPCR). J Microbiol 2007; 45 453–9.
| 1:CAS:528:DC%2BD2sXhsVanu7%2FL&md5=f95ca2983fb6313e1a35ae805e0396bcCAS |

[2]  Fredricks D, Relman D. Paraffin removal from tissue sections for digestion and PCR analysis. Biotechniques 1999; 26 198–200.
| 1:CAS:528:DyaK1MXhtFGku7w%3D&md5=05364df79c958f0a9f52e082e57162d1CAS |

[3]  McIver CJ, Rismanto N, Smith C, Naing ZW, Rayner B, Lusk MJ, et al Multiplex PCR testing detection of higher-than-expected rates of cervical Mycoplasma, Ureaplasma, and Trichomonas and viral agent infections in sexually active Australian women. J Clin Microbiol 2009; 47 1358–63.
Multiplex PCR testing detection of higher-than-expected rates of cervical Mycoplasma, Ureaplasma, and Trichomonas and viral agent infections in sexually active Australian women.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXmt1Cqsr0%3D&md5=f92ed3ec1592f5c068ced2f2991d1571CAS |

[4]  Baczynska A, Hvid M, Lamy P, Birkelund S, Christiansen G, Fedder J. Prevalence of Mycoplasma genitalium, Mycoplasma hominis and Chlamydia trachomatis among Danish patients requesting abortion. Syst Biol Reprod Med 2008; 54 127–34.
Prevalence of Mycoplasma genitalium, Mycoplasma hominis and Chlamydia trachomatis among Danish patients requesting abortion.Crossref | GoogleScholarGoogle Scholar |

[5]  Rosenstein IJ, Morgan DJ, Sheehan M, Lamont RF, Taylor-Robinson D. Bacterial vaginosis in pregnancy: distribution of bacterial species in different Gram-stain categories of the vaginal flora. J Med Microbiol 1996; 44 1–7.

[6]  Mardh PA, Novikova N. Chlamydia trachomatis infections – a major concern for reproductive health. Where do we stand regarding epidemiology, pathogenesis, diagnosis and therapy? Eur J Contracept Reprod Health 2001; 6 115–26.
| 1:STN:280:DC%2BD3MvnsVKlsw%3D%3D&md5=40361bb6d88eb291cdeba986d5ec78cdCAS |

[7]  Maeda SI, Deguchi T, Ishiko H, Matsumoto T, Naito S, Kumon H, et al Detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum (biovar 1) and Ureaplasma urealyticum (biovar 2) in patients with non-gonococcal urethritis using polymerase chain reaction-microtiter plate hybridization. Int J Urol 2004; 11 750–4.
Detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum (biovar 1) and Ureaplasma urealyticum (biovar 2) in patients with non-gonococcal urethritis using polymerase chain reaction-microtiter plate hybridization.Crossref | GoogleScholarGoogle Scholar |

[8]  Simou M. Prevalence of Mycoplasma in female genital tract and susceptibility to antimicrobial agents. 14th European Congress of Clinical Microbiology and Infectious Diseases; Prague/Czech Republic, 2004, May 1–4; abstract number: 902_p1598.

[9]  Baka S, Kouskouni E, Antonopoulou S, Sioutis D, Papakonstantinou M, Hassiakos D, et al Prevalence of Ureaplasma urealyticum and Mycoplasma hominis in women with chronic urinary symptoms. Urology 2009; 74 62–6.
Prevalence of Ureaplasma urealyticum and Mycoplasma hominis in women with chronic urinary symptoms.Crossref | GoogleScholarGoogle Scholar |

[10]  Zdrodowska-Stefanow B, Kłosowska WM, Ostaszewska-Puchalska I, Bułhak-Kozioł V, Kotowicz B. Ureaplasma urealyticum and Mycoplasma hominis infection in women with urogenital diseases. Adv Med Sci 2006; 51 250–3.
| 1:STN:280:DC%2BD2s7lslKrsg%3D%3D&md5=6694f78b5a1dacf92bbf7cd89edae758CAS |

[11]  Dhawan B, Gupta V, Khanna N, Singh M, Chaudhry R. Evaluation of the diagnostic efficacy of PCR for Ureaplasma urealyticum infection in Indian adults with symptoms of genital discharge. Jpn J Infect Dis 2006; 59 57–8.

[12]  Karabay O, Topcuoglu A, Kocoglu E, Gurel S, Gurel H, Ince NK. Prevalence and antibiotic susceptibility of genital Mycoplasma hominis and Ureaplasma urealyticum in a university hospital in Turkey. Clin Exp Obstet Gynecol 2006; 33 36–8.
| 1:STN:280:DC%2BD28zkvVWksg%3D%3D&md5=a0e219d6aff016c361e7d87d3beb494aCAS |

[13]  Nassar FA, Abu-Elamreen FH, Shubair ME, Sharif FA. Detection of Chlamydia trachomatis and Mycoplasma hominis, genitalium and Ureaplasma urealyticum by polymerase chain reaction in patients with sterile pyuria. Adv Med Sci 2008; 53 80–6.
Detection of Chlamydia trachomatis and Mycoplasma hominis, genitalium and Ureaplasma urealyticum by polymerase chain reaction in patients with sterile pyuria.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXht12hs7%2FP&md5=37edb386b055910c2c6f61158a2777fdCAS |

[14]  Olsen B, Lan PT, Stalsby Lundborg C, Khang TH, Unemo M. Population-based assessment of Mycoplasma genitalium in Vietnam – low prevalence among married women of reproductive age in a rural area. J Eur Acad Dermatol Venereol 2009; 23 533–7.
Population-based assessment of Mycoplasma genitalium in Vietnam – low prevalence among married women of reproductive age in a rural area.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD1MzgslSisg%3D%3D&md5=36c5b45a118197c99ac78af61c243e88CAS |

[15]  Andersen B, Sokolowski I, Ostergaard L, Møller JK, Olesen F, Jensen JS. Mycoplasma genitalium: prevalence and behavioural risk factors in the general population. Sex Transm Infect 2007; 83 237–41.
Mycoplasma genitalium: prevalence and behavioural risk factors in the general population.Crossref | GoogleScholarGoogle Scholar |

[16]  Moi H, Reinton N, Moghaddam A. Mycoplasma genitalium is associated with symptomatic and asymptomatic non-gonococcal urethritis in men. Sex Transm Infect 2009; 85 15–8.
Mycoplasma genitalium is associated with symptomatic and asymptomatic non-gonococcal urethritis in men.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD1M%2Fos12hsw%3D%3D&md5=b73b59b3078e0421fbd518e443bcb47aCAS |

[17]  Rahman S, Garland S, Currie M, Tabrizi SN, Rahman M, Nessa K, et al Prevalence of Mycoplasma genitalium in health clinic attendees complaining of vaginal discharge in Bangladesh. Int J STD AIDS 2008; 19 772–4.
Prevalence of Mycoplasma genitalium in health clinic attendees complaining of vaginal discharge in Bangladesh.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD1cnnt1yluw%3D%3D&md5=70e61f3bf1828223f05e0b54cc71bf04CAS |

[18]  Jensen JS, Rnelius EB, Dohn B, Lidbrink P. Comparison of first void urine and urogenital swab specimens for detection of Mycoplasma genitalium and Chlamydia trachomatis by polymerase chain reaction in patients attending a sexually transmitted disease clinic. Sex Transm Dis 2004; 31 499–507.
Comparison of first void urine and urogenital swab specimens for detection of Mycoplasma genitalium and Chlamydia trachomatis by polymerase chain reaction in patients attending a sexually transmitted disease clinic.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXlvV2ru78%3D&md5=92347da8d676b832d8c4297ba46af785CAS |

[19]  Anagrius C, Loré B, Jensen JS. Mycoplasma genitalium: prevalence, clinical significance, and transmission. Sex Transm Infect 2005; 81 458–62.
Mycoplasma genitalium: prevalence, clinical significance, and transmission.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2MnksleitA%3D%3D&md5=3903322735c28e59f67c737d1425b4b4CAS |

[20]  Museva A, Shopova E, Dimitrov A, Nikolov A. Participation of the genital mycoplasmas: Ureaplasma urealyticum and Mycoplasma hominis in the processes of preterm birth. Akush Ginekol 2007; 46 12–5.