43. Anal human papillomavirus prevalence in HIV-negative and HIV-infected Russian men who have sex with women
Vitaly Smelov A B , Miriam Elfström B , Carina Eklund B , Olga Sokolova C D , Andrey Novikov A , Boris Komyakov A and Joakim Dillner BA Karolinska Institutet, Stockholm, Sweden.
B North-Western State Medical University named after I.I. Mechnikov, Department of Urology and Andrology, St Petersburg, Russia.
C S. P. Botkin Memorial Clinical Hospital of Infectious Diseases, St Petersburg, Russia.
D St Petersburg State University, St Petersburg, Russia.
Sexual Health 10(6) 591-591 https://doi.org/10.1071/SHv10n6ab43
Published: 22 November 2013
Abstract
Background: HPV has been found in the anal canal of heterosexual men (MSWs) but knowledge on anal HPV epidemiology among MSWs is still limited. So far, there are no such data from Russia. Methods: In total, 350 β-globin-positive anal samples from Russian MSWs (age 18–58 years; sex debut 9–23 years; 1–700 life-time sex partners) attending a urology unit of an STI clinic and HIV+ patients from a city infection hospital were collected in St Petersburg. PCR using HPV general primers with genotyping using Luminex with probes for 13 oncogenic, high-risk HPV types (16, 18, 31, 33, 35 39, 45, 51, 52, 56, 58, 59, and 68, including variants) and 23 non-oncogenic types (6, 11, 26, 30, 40, 42, 43, 53, 54, 61, 66, 67, 70, 73, 74, 81, 82, 83, 86, 87, 89, 90, and 91) was used with 11 negative controls (water) and 8 positive controls (HPV plasmid pools) run in each assay. The method was proficient in the WHO HPV LabNet global proficiency panel. Results: Overall, HPV prevalence (including oncogenic and non-oncogenic types) was 17.1%, with 15.2% in HIV– compared with 40.7% in HIV+ Russian MSW (P = 0.0022). The most commonly detected was HPV-16 (5.4%) followed by HPV-51 (2.0%), HPV-45, (1.7%) and HPV-87 (1.7%). Interestingly, neither age, nor number of sexual partners, nor age at sexual debut were associated with HPV infection. Conclusions: HPV infection is common in anal canal samples of Russian MSWs. HPV prevalence is higher among men who were HIV positive.
