34. One lesion, one virus: individual components of high-grade anal intraepithelial neoplasia in HIV+ men contain a single HPV type
O. Richel A , K. D. Quint B , J. Lindeman B , C. M. Van Noesel A , M. N. C. De Koning B , H. A. M. Van den Munckhof B , H. J. C. De Vries A , J. M. Prins A and W. G. V. Quint BA Academic Medical Center, Amsterdam, The Netherlands.
B DDL Diagnostic Laboratory, Rijswijk, The Netherlands.
Sexual Health 10(6) 586-587 https://doi.org/10.1071/SHv10n6ab34
Published: 22 November 2013
Abstract
Background: Anal intraepithelial neoplasia (AIN) is present in the majority of HIV+ men who have sex with men (MSM). The main aetiological factor is human papilloma virus (HPV). We analysed whether HPV PCR genotyping of whole tissue sections (WTS) and subsequent laser capture microdissection (LCM) detects type-specific HPV DNA in individual areas of HGAIN, and compared these lesional HPV genotypes with HPV types in anal swabs. Methods: DNA was isolated from anal swabs and WTS of 31 HGAIN biopsies obtained from 21 HIV+ MSM and analysed by the SPF10 PCR/LiPA25 HPV genotyping system. In case of multiple HPV types per WTS, PCR was repeated in selected areas of AIN obtained by LCM. Results: A single HPV type was observed in 15 (48%) of 31 WTS. In the 16 WTS with multiple HPV types, LCM-isolated dysplastic areas were studied. In 14 of the 16 WTS, one single HPV type was demonstrated within each discrete area of the lesion, resulting in a total of 29 (15+14) biopsies in which a single HPV type was found in (all components of) the lesion. HPV 16 was found in 14 of 31 biopsies (45%). In almost half the biopsies, lesional HPV types were not detected in the anal swabs. Conclusions: WTS PCR and subsequent LCM PCR is accurate in detecting lesion-specific HPV types in HGAIN and in 94% of the biopsies a single HPV was found in (each component of) the HGAIN lesion. In the majority, HPV16 was not the causative type and HPV genotyping of anal swabs was not useful in detecting the lesion-specific type.
