Peroxisome proliferator-activated receptor delta-PPARδ agonist (L-165041) enhances bovine embryo survival and post vitrification viability
Jesús Alfonso Sánchez Viafara
A B * , Gisvani Lopes de Vasconcelos C , Renata Maculan D , Nadja Gomes Alves C , Marcos Brandao Dias Ferreira E , Mateus José Sudano F , Gisele Zoccal Mingoti G , Giovana Barros Nunes G , Renato Ribeiro de Lima H , Roberti Martins Drumond I , Raphael Nunes dos Santos I , Marcos Nogueira Eberlin J , Fernanda Negrão J ,
+ Author Affiliations
- Author Affiliations
A Departamento de Medicina Veterinária, Universidade Federal de Lavras, Lavras, Minas Gerais, Brasil.
B Universidad de Santander, Facultad de Ciencias Agrícolas y Veterinarias, Valledupar, Colombia.
C Departamento de Zootecnia, Universidade Federal de Lavras, Lavras, Minas Gerais, Brasil.
D Instituto Federal do Sul de Minas, Machado, Minas Gerais, Brasil.
E EPAMIG, Belo Horizonte, Minas Gerais, Brasil.
F Universidade Federal do ABC, Santo André, São Paulo, Brasil.
G Escola de Medicina Veterinária, Laboratório de Fisiologia da Reprodução, Universidade Estadual Paulista, Campus Araçatuba, São Paulo, Brasil.
H Departamento de Estatística, Universidade Federal de Lavras, Lavras, Minas Gerais, Brasil.
I Cenatte Embriões, Pedro Leopoldo, Minas Gerais, Brasil.
J Universidade Estadual de Campinas, Laboratório ThoMSon de Espectrometria de Massas, Campinas, São Paulo, Brasil.
K NUMPEX-Bio, Universidade Federal do Rio de Janeiro, Campus Duque de Caxias, Rio de Janeiro, Brasil.
L Centro de Pesquisas Aggeu Magalhães, Fiocruz/Pernambuco, Brasil.
Reproduction, Fertility and Development 34(9) 658-668 https://doi.org/10.1071/RD21245
Published online: 26 April 2022
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
The effect of L-165041 (PPARδ-agonist) on decreasing apoptosis and intracellular lipid content was assessed in fresh and vitrified–warmed in vitro-produced bovine embryos. It was hypothesised that the addition of L-165041 to the culture medium enhances development and cryopreservation. Oocytes were allocated to one of two treatments: control-standard culture medium, or L-165041 added to the medium on day 1 with no media change. Ultrastructure, cleavage, and blastocyst rates were evaluated in fresh, and in post-vitrification cultured embryos by optical and electronic microscopy. A subset of fresh embryos were fixed for TUNEL assay and for Sudan-Black-B histochemical staining. Vitrified–warmed embryos were assessed using MALDI-MS technique. Cleavage and blastocyst rates (control 49.4 ± 5.2, L-165041 51.8 ± 4.3) were not influenced by L-165041. The proportion of inner cell mass cells (ICM) was higher in fresh embryos, and the rate of total and ICM apoptosis was lower in L-165041. In warmed-embryos, total and ICM apoptosis was lower in L-165041. The overall hatching rate was higher in L-165041 (66.62 ± 2.83% vs 53.19 ± 2.90%). There was less lipid accumulation in fresh L-165041-embryos. In conclusion, the use of L-165041 is recommended to improve the viability of in vitro-derived bovine embryos.
Keywords: bovine, cryopreservation, culture medium, development, embryo apoptosis, embryos, lipid, vitrification.
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