Effects of thyroid hormone on ovarian cell apoptosis in the rat
Qiaozhi Wang A , Xiaoshu Ma A and Cheng Zhang
A B
+ Author Affiliations
- Author Affiliations
A College of Life Science, Capital Normal University, Beijing 100048, P.R. China.
B Corresponding author. Email: zhch8012@163.com
Reproduction, Fertility and Development 32(12) 1060-1066 https://doi.org/10.1071/RD20110
Submitted: 18 April 2020 Accepted: 18 June 2020 Published: 13 July 2020
Abstract
Follicle development is a complicated process regulated by thyroid hormone (TH). TH dysregulation is associated with reproductive disorders; however, the mechanism underlying these relationships remains unclear. Glucose-related protein 78 (GRP78) is a well-characterised endoplasmic reticulum stress protein related to ovarian cell apoptosis. To clarify whether GRP78 is regulated by TH and the involvement of GRP78 in follicle development, we established rat models of hypothyroidism and hyperthyroidism and investigated the effects of TH dysregulation on levels of GRP78, C/EBP homologous protein (CHOP) and cleaved caspase-3. TH dysregulation decreased levels of GRP78 and increased those of CHOP and cleaved caspase-3 in both rat models. However, treatment with equine chorionic gonadotrophin reversed these effects, as well as granulosa cell apoptosis induced by TH dysregulation. Together, these results provide evidence that TH dysregulation alters the GRP78 expression profile, triggering the apoptotic signalling pathway, and suggest that GRP78 is a novel mediator of TH in follicle development.
Additional keywords: glucose-related protein 78 (GRP78), ovary.
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