Effects of 2,3′,4,4′,5-pentachlorobiphenyl exposure during pregnancy on DNA methylation in the testis of offspring in the mouse
Jian-Mei Zhang A * , Qiu-Yue Wang A * , Xiao-Ying Han A , Qi-Long He A , Li Liu A , Yong-Tao Zhang A , Xiao-Qian Meng A , Dong Cheng B , Tian-Liang Zhang B and Shu-Zhen Liu![https://orcid.org/0000-0002-5440-679X](/media/client/orcid_16x16.png)
A Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Sciences, Shandong Normal University, Jinan 250014, China.
B Department of Toxicology, Shandong Center for Disease Control and Prevention, Jinan 250014, China.
C Corresponding author. Email: shuzhen26@163.com
Reproduction, Fertility and Development 32(12) 1048-1059 https://doi.org/10.1071/RD19412
Submitted: 29 October 2019 Accepted: 22 May 2020 Published: 31 July 2020
Abstract
Polychlorinated biphenyls (PCBs) are persistent organic pollutants, and the widespread use of PCBs has had adverse effects on human and animal health. This study experiment explored the effects of 2,3′,4,4′,5-pentachlorobiphenyl (PCB118) on the mammalian reproductive system. PCB118 was administered to pregnant mice from 7.5 to 12.5 days of gestation; F1 mice were obtained and the reproductive system of F1 male mice was examined. PCB118 damaged the reproductive system in male F1 mice, as evidenced by negative effects on the testicular organ coefficient (testes weight/bodyweight), a decrease in the diameter of seminiferous tubules and a significant reduction in the anogenital distance in 35-day-old F1 mice. In addition, methylation levels of genomic DNA were reduced, with reductions in the expression of the DNA methyltransferases DNMT1, DNMT3A and DNMT3B, as well as that of the epigenetic regulatory factor ubiquitin like with PHD and ring finger domains 1 (Uhrf1). Together, the results of this study provide compelling evidence that exposure of pregnant mice to PCB118 during primordial germ cell migration in the fetus affects the reproductive system of the offspring and decreases global methylation levels in the testis.
![Graphical Abstract Image](/temp/RD19412_toc.gif)
Additional keywords: DNA methyltransferase, PCB118.
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