Small RNA (sRNA) expression in the chorioallantois, endometrium and serum of mares following experimental induction of placentitis
Shavahn C. Loux A , Claudia B. Fernandes B , Pouya Dini A , Kai Wang C , Xiaogang Wu C , David Baxter C , Kirsten E. Scoggin A , Mats H. T. Troedsson A , Edward L. Squires A and Barry A. Ball
A D
+ Author Affiliations
- Author Affiliations
A Department of Veterinary Science, University of Kentucky, 1400 Nicholasville Road, Lexington, KY 40546, USA.
B Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof Dr Orlando Marques de Paiva, 87, 05508-270, São Paulo, SP, Brazil.
C Institute for Systems Biology, Seattle, WA 98109, USA.
D Corresponding author. Email: b.a.ball@uky.edu
Reproduction, Fertility and Development 31(6) 1144-1156 https://doi.org/10.1071/RD18400
Submitted: 4 October 2018 Accepted: 30 January 2019 Published: 5 April 2019
Abstract
Intrauterine infection and inflammation remain a major cause of preterm labour in women and mares, with little known about small RNA (sRNA) expression in tissue or circulation. To better characterise placental inflammation (placentitis), we examined sRNA expression in the endometrium, chorioallantois and serum of mares with and without placentitis. Disease was induced in 10 mares via intracervical inoculation of Streptococcus equi ssp. zooepidemicus, either with moderate or high levels of inoculum; three uninoculated gestationally matched mares were used as controls. Matched chorioallantois and endometrium were sampled in two locations: Region 1, gross inflammation near cervical star with placental separation and Region 2, gross inflammation without placental separation. In Region 1, 26 sRNAs were altered in chorioallantois, while 20 were altered in endometrium. Within Region 2, changes were more subdued in both chorioallantois (10 sRNAs) and endometrium (two sRNAs). Within serum, we identified nine significantly altered sRNAs. In summary, we have characterised the expression of sRNA in the chorioallantois, the endometrium and the serum of mares with experimentally induced placentitis using next-generation sequencing, identifying significant changes within each tissue examined. These data should provide valuable information about the physiology of placental inflammation to clinicians and researchers alike.
Additional keywords: infection, inflammation, microRNA, placenta, pregnancy.
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