Caveolin-1 promotes trophoblast cell invasion through the focal adhesion kinase (FAK) signalling pathway during early human placental development
Zhihui Dai
A * , Fei Sheng B * , Ningxia Sun B , Yixuan Ji B , Qiuying Liao A , Shuhan Sun A , Fu Yang A C and Wen Li B C
+ Author Affiliations
- Author Affiliations
A Department of Medical Genetics, Second Military Medical University, Shanghai, 200433, China.
B Centre of Reproductive Medicine, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China.
C Corresponding authors. Emails: yangfusq1997@smmu.edu.cn; liwen@smmu.edu.cn
Reproduction, Fertility and Development 31(6) 1057-1067 https://doi.org/10.1071/RD18296
Submitted: 30 July 2018 Accepted: 7 January 2019 Published: 4 April 2019
Abstract
Normal implantation and placental development depend on the appropriate differentiation and invasion of trophoblast cells. Inadequate trophoblast cell invasion results in pregnancy-related disorders, which endanger both mother and fetus; however, the mechanism of early placental development has not been fully explained. In this study we conducted gene expression profile analysis using mouse placental tissues at different developmental stages (embryonic day (E)7.5, E14.5 and E19.5) using series tests of cluster (STC) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analyses. Focal adhesion kinase (FAK) signalling pathway-related gene expression levels were verified using quantitative reverse transcription polymerase chain reaction and western blot. The results showed that caveolin-1 (Cav1) was downregulated in the placenta of unexplained spontaneous abortion subjects compared with that of induced abortion. Furthermore, by modulating CAV1 expression levels, CAV1 was shown to promote human trophoblast cell proliferation, migration and invasion by activating the FAK signalling pathway. These results indicate that CAV1 and the FAK signalling pathway are crucial for early placental development, which sheds new light on our understanding of the mechanisms of human trophoblast cell invasion and early development of the placenta.
Additional keywords: CAV1, gene expression profile, placenta, spontaneous abortion.
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