Predicted gene 31453 (Gm31453) and the gene encoding carboxypeptidase A5 (Cpa5) are not essential for spermatogenesis and male fertility in the mouse
Yang Zhou A * , Xiaona Zhang B * , Suping Xiong C , Xuhui Zeng A D and Xiaoning Zhang
A C D
+ Author Affiliations
- Author Affiliations
A Institute of Reproductive Medicine, Medical School, Nantong University, Nantong 226019, PR China.
B Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, PR China.
C Institute of Life Science, Nanchang University, Nanchang 330031, PR China.
D Corresponding authors. Email: zengxuhui@ntu.edu.cn; zhangxn@ntu.edu.cn
Reproduction, Fertility and Development 33(6) 401-409 https://doi.org/10.1071/RD20284
Submitted: 30 October 2020 Accepted: 9 February 2021 Published: 22 March 2021
Abstract
Numerous long non-coding (lnc) RNAs are highly enriched or exclusively expressed in the mammalian testis, even in spermatids. Spermatid perinuclear RNA-binding protein (STRBP) can bind many RNAs, and loss of STRBP impairs male fertility. However, the functions of lncRNAs interacting with STRBP are unknown. In this study, the roles of one STRBP-interacting lncRNA, namely predicted gene 31453 (Gm31453), and its potential target gene encoding carboxypeptidase A5 (Cpa5) in spermatogenesis were determined using gene-knockout (KO) mice. Gm31453 and Cpa5 are located adjacent to each other on the same chromosome and are highly expressed in the testis. Gm31453 and Cpa5 are primarily expressed from secondary spermatocytes to elongated spermatids, implying their involvement in spermiogenesis. Although deletion of Gm31453 disturbed the expression of both its target and interacting gene, as indicated by decreased Cpa5 and increased Strbp mRNA levels, both Gm31453- and Cpa5-KO mice showed normal spermatogenesis and fertility, and had no detectable abnormalities in terms of testicular and epididymal development, sperm production morphology or motility, pregnancy rate or litter size. Thus, Gm31453 and Cpa5 are dispensable for spermatogenesis and male fertility in mice. Their involvement in spermatogenesis may be a fine-tuning role, regulating gene expression at the molecular level.
Keywords: Cpa5, Gm31453, lncRNA, male fertility, spermatogenesis, Strbp.
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