190 Evaluation of the toxicity of different concentrations of dimethyl sulfoxide and ethylene glycol cryoprotectants on immature cattle oocytes selected by Brilliant cresyl blue stain and subsequent to polar body extrusion

M. L. Mphaphathi; M. R. Ledwaba; M. D. Sebopela; M. A. Thema; T. L. Mashilo; H. A. O’Neill; T. L. Nedambale

doi:10.1071/RDv37n1Ab190

RESEARCH ARTICLE

190 Evaluation of the toxicity of different concentrations of dimethyl sulfoxide and ethylene glycol cryoprotectants on immature cattle oocytes selected by Brilliant cresyl blue stain and subsequent to polar body extrusion

M. L. Mphaphathi ^A , M. R. Ledwaba ^A ^B , M. D. Sebopela ^A , M. A. Thema ^A , T. L. Mashilo ^B ^C , H. A. O’Neill ^B and T. L. Nedambale ^D

+ Author Affiliations

- Author Affiliations

^A Agricultural Research Council, Animal Production, Germplasm Conservation & Reproduction Biotechnologies, Pretoria, RSA

^B University of the Free State, Department of Animal, Wildlife and Grassland Sciences, Bloemfontein, RSA

^C Department of Agriculture, Land Reform, and Rural Development, Directorate: Animal Production, Sub-Directorate: Animal Improvement, Arcadia, Pretoria, RSA

^D Tshwane University of Technology, Department of Animal Science, Pretoria, RSA

Reproduction, Fertility and Development 37, RDv37n1Ab190 https://doi.org/10.1071/RDv37n1Ab190