30 Bull sperm kinetics after semen cryopreservation in extender containing propagermanium
T. E. Cruz A , A. MartinsA University of São Paulo State, Botucatu, SP, Brazil;
B University of São Paulo State, Araçatuba, SP, Brazil;
C MasterFertility Ltda, Araçatuba, SP, Brazil;
D Artificial Insemination Station, Presidente Prudente, SP, Brazil
Reproduction, Fertility and Development 31(1) 141-141 https://doi.org/10.1071/RDv31n1Ab30
Published online: 3 December 2018
Abstract
There is a negative association between a plentiful production of oxygen reactive species and spermatozoa kinetics parameters. Thus, antioxidants have been added to the freezing medium to improve sperm quality due to their protective effect against membrane lipid peroxidation. Propagermanium (GE132) is an organometallic compound that has never been used in freezing medium despite its known antioxidant effect as a free radical scavenger. This study aimed to investigate the effects of different concentrations of GE132 added in a commercial freezing medium (CFM) on frozen-thawed sperm motion. Nine ejaculates of 3 Nellore bulls (3 replicates), collected by an artificial vagina, were evaluated, pooled, and divided into groups D (semen was diluted and kept at 33°C for 30 min before cooling) and C (semen was cooled immediately after dilution). Both groups were submitted to the same experimental treatment, as follows: addition of 0, 500, and 1000 µg mL−1 of GE132 in a CFM resulting in subgroups D0, D500, D1000, C0, C500, and C1000. The sperm samples were diluted to a final concentration of 30 × 106 spermatozoa per straw (0.25 mL) and then cooled at 4°C for 5 h before freezing. Sperm samples were assessed using a computer assisted sperm analyser at 5 and 60 min post-thawing for total motility (TM; %), progressive motility (%), curvilinear velocity (μm s−1), velocity straight line (μm s−1), velocity average path (μm s−1), amplitude of the lateral head displacement (ALH; μm), beat cross frequency (Hz), linearity (LIN; %), and straightness (STR; %). Data were analysed using the R software package version 3.4.4 (2018;
We acknowledge Tairana AI Station, Master Fertility, and Botupharma, Brazil.