Induction of right open reading frame kinase 3 (RIOK3) during ovulation and luteinisation in rat ovary
Wei Zhang A , Chujing Zhang A , Ruiqi Huang A , Mengsheng Qiu A and Fei-xue Li
A *
+ Author Affiliations
- Author Affiliations
A Zhejiang Key Laboratory of Organ Development and Regeneration, Institute of Developmental and Regenerative Biology, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
Reproduction, Fertility and Development 33(16) 810-816 https://doi.org/10.1071/RD21118
Published online: 11 November 2021
© 2021 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
Atypical protein serine kinase RIOK3 is involved in cellular invasion and survival. The spatiotemporal expression pattern and regulatory mechanisms controlling expression of Riok3 were investigated in the rat ovary during the periovulatory period. Immature female rats (22–23 days old) were treated with pregnant mare’s serum gonadotropin (PMSG) to stimulate follicular development, followed 48 h later by injection with human chorionic gonadotrophin (hCG). Ovaries, granulosa cells, or theca-interstitial cells were collected at various times after hCG administration. Both real-time polymerase chain reaction (PCR) and in situ hybridisation analysis revealed that Riok3 was highly induced in both granulosa cells and theca-interstitial cells by hCG. Riok3 expression was induced in theca-interstitial cells at 4 h after hCG. However, the expression of Riok3 mRNA was stimulated in granulosa cells at 8 h. Both protein kinase C inhibitor (GF109203) and the protein kinase A inhibitor (H89) could block the stimulation of Riok3 mRNA by hCG. Furthermore, Riok3 induction is dependent on new protein synthesis. Inhibition of prostaglandin synthesis or progesterone action did not alter Riok3 mRNA expression, whereas inhibition of the epidermal growth factor (EGF) pathway downregulated Riok3 expression. In conclusion, our findings suggest that the induction of the RIOK3 may be important for ovulation and luteinisation.
Keywords: gene expression, granulosa cell, LH, luteinisation, ovary, ovulation, RIOK3, signal pathway.
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