The cyclin-dependent kinase inhibitor, JNJ-7706621, improves in vitro developmental competence of porcine parthenogenetic activation and somatic cell nuclear transfer embryos
Qing Guo A , Long Jin A , Hai-Ying Zhu A , Xiao-Xu Xing A , Mei-Fu Xuan A , Qi-Rong Luo A , Guang-Lei Zhang A , Zhao-Bo Luo A , Jun-Xia Wang A , Xi-Jun Yin A B and Jin-Dan Kang A B
+ Author Affiliations
- Author Affiliations
A Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, Jilin, 133002, China.
B Corresponding authors. Emails: yinxj33@msn.com; kangjindan@hotmail.com
Reproduction, Fertility and Development 30(7) 1002-1010 https://doi.org/10.1071/RD17194
Submitted: 22 May 2017 Accepted: 18 November 2017 Published: 5 January 2018
Abstract
In this study we examined the effects of JNJ-7706621, a cyclin-dependent kinase inhibitor, on the in vitro growth of pig embryos that had been produced either by parthenogenetic activation (PA) or somatic cell nuclear transfer (SCNT). A significantly higher percentage of PA embryos reached the blastocyst stage by Day 7 after exposure to 10 µM JNJ-7706621 for 4 h compared with embryos exposed to 5 µg mL−1 cytochalasin B for 4 h (P < 0.05). Similarly, the rate of Tyr15 phosphorylation of the complex of cyclin and p34cdc2 (CDK1) was significantly elevated in the JNJ-7706621-treated embryos compared with embryos exposed to cytochalasin B or non-treated controls (P < 0.05). In contrast, Thr161 phosphorylation of CDK1 was significantly lower in the JNJ-7706621-treated group compared with the cytochalasin B-treated as well as the non-treated group (P < 0.05). Similarly, the level of M-phase-promoting factor (MPF) in embryos was significantly lower in the JNJ-7706621-treated group compared with the cytochalasin B-treated and non-treated groups (P < 0.05). In addition, more SCNT embryos reached the blastocyst stage after treatment with JNJ-7706621 than following exposure to cytochalasin B (P < 0.05). In conclusion, these results reveal that exposure to 10 µM JNJ-7706621 for 4 h improves early development of PA and SCNT porcine embryos by suppressing the activity of CDK1 and a concomitant reduction in the level of MPF.
Additional keywords: blastocyst, CDK1, cytochalasin B, embryos, M-phase-promoting factor.
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