Linoleic (LA) and linolenic (ALA) acid concentrations in follicular fluid of prepubertal goats and their effect on oocyte in vitro maturation and embryo development
Montserrat Roura A , María G. Catalá A , Sandra Soto-Heras A , Sondes Hammami A , Dolors Izquierdo A , Ali Fouladi-Nashta B and Maria-Teresa Paramio A C
+ Author Affiliations
- Author Affiliations
A Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona (UAB), Travessera dels Turons s/n, 08193, Bellaterra, Barcelona, Catalonia, Spain.
B Reproduction Genes and Development Group, Department of Veterinary Basic Sciences, The Royal Veterinary College, Hawkshead Lane Hatfield, Herts AL97TA, UK.
C Corresponding author. Email: teresa.paramio@uab.cat
Reproduction, Fertility and Development 30(2) 286-296 https://doi.org/10.1071/RD17174
Submitted: 7 February 2017 Accepted: 7 June 2017 Published: 6 July 2017
Abstract
In this study we assessed the concentration of linoleic acid (LA) and linolenic acid (ALA) in follicular fluid of prepubertal goats according to follicle size (<3 mm or ≥3 mm) by gas chromatography and tested the addition of different LA and ALA (LA : ALA) concentration ratios (50 : 50, 100 : 50 and 200 : 50 µM) to the IVM medium on embryo development, mitochondrial activity, ATP concentration and relative gene expression (RPL19, ribosomal protein L19; SLC2A1, facilitated glucose transporter 1; ATF4, activating transcription factor 4; GPX1, glutathione peroxidase 1; HSPA5, heat-shock protein family A 70 kDa; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; DNMT1, DNA methyltransferase 1; GCLC, glutamate–cysteine ligase catalytic subunit; SOD1, superoxide dismutase 1). Oocytes were in vitro matured, fertilised or parthenogenetically activated and zygotes were cultured following conventional protocols. LA concentration ranged from 247 to 319 µM and ALA concentration from 8.39 to 41.19 µM without any effect of follicle size. Blastocyst production from the different groups was: control FCS (22.33%) and BSA (19.63%), treatments 50 : 50 (22.58%), 100 : 50 (21.01%) and 200 : 50 (9.60%). Oocytes from the 200 : 50 group presented higher polyspermy and mitochondrial activity compared with controls and the rest of the treatment groups. No differences were observed in ATP concentration or relative expression of the genes measured between treatment groups. In conclusion, the low number of blastocysts obtained in the 200 : 50 group was caused by a high number of polyspermic zygotes, which could suggest that high LA concentration impairs oocyte membranes.
Additional keywords: blastocysts, embryo production, omega-3 and omega-6 PUFAs, oocyte membrane.
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