Expanded equine cumulus–oocyte complexes exhibit higher meiotic competence and lower glucose consumption than compact cumulus–oocyte complexes
L. González-Fernández A B , M. J. Sánchez-Calabuig C , M. G. Alves D , P. F. Oliveira D , S. Macedo A , A. Gutiérrez-Adán C , A. Rocha A and B. Macías-García A E F
+ Author Affiliations
- Author Affiliations
A Centro de Estudos de Ciência Animal/Instituto de Ciências, Tecnologias e Agroambiente; Instituto de Ciências Biomédicas Abel Salazar, University of Porto, Campus Agrario de Vairão, Rua Padre Armando Quintas, 4485-661 Vairão, Portugal.
B Research Group of Intracellular Signalling and Technology of Reproduction (SINTREP), School of Veterinary Medicine, University of Extremadura, Avda. de la universidad s/n, 10003, Cáceres, Spain.
C Department of Animal Reproduction, Instituto nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra. De la Coruña, Km. 5.9, Madrid 28040, Spain.
D Department of Microscopy, Cell Biology Laboratory, Abel Salazar Institute of Biomedical Sciences and Unit for Multidisciplinary Research in Biomedicine (UMIB), University of Porto, Porto, Portugal.
E Assisted Reproduction Unit, Jesús Usón Minimally Invasive Surgery Centre (CCMIJU), Carretera N-521, km. 41,8. 10071, Cáceres, Spain.
F Corresponding author. Email: bea_macias@hotmail.com
Reproduction, Fertility and Development 30(2) 297-306 https://doi.org/10.1071/RD16441
Submitted: 4 November 2016 Accepted: 6 June 2017 Published: 6 July 2017
Abstract
Equine cumulus–oocyte complexes (COCs) are classified as compact (cCOC) or expanded (eCOC) and vary in their meiotic competence. This difference could be related to divergent glucose metabolism. To test this hypothesis in the present study, eCOCs, cCOCs and expanded or compact mural granulosa cells (EC and CC respectively) were matured in vitro for 30 h, at which time maturation rate, glucose metabolism and the expression of genes involved in glucose transport, glycolysis, apoptosis and meiotic competence were determined. There were significant differences between eCOCs and cCOCs in maturation rate (50% vs 21.7% (n = 192 and 46) respectively; P < 0.001), as well as mean (± s.e.m.) glucose consumption (1.8 ± 0.5 vs 27.9 ± 5.9 nmol per COC respectively) and pyruvate (0.09 ± 0.01 vs 2.4 ± 0.8 nmol per COC respectively) and lactate (4.7 ± 1.3 vs 64.1 ± 20.6 nmol per COC respectively; P < 0.05 for all) production. Glucose consumption in EC and CC did not differ significantly. Expression of hyaluronan-binding protein (tumour necrosis factor alpha induced protein 6; TNFAIP6) was increased in eCOCs and EC, and solute carrier family 2 member 1 (SLC2A1) expression was increased in eCOCs, but there were no differences in the expression of glycolysis-related enzymes and solute carrier family 2 member 3 (SLC2A3) between the COC or mural granulosa cell types. The findings of the present study demonstrate that metabolic and genomic differences exist between eCOCs and cCOCs and mural granulosa cells in the horse.
Additional keywords: glycolysis, horse, in vitro maturation, nuclear magnetic resonance.
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