Effects of dietary quercetin on female fertility in mice: implication of transglutaminase 2
Kelly E. Beazley A and Maria Nurminskaya A B
+ Author Affiliations
- Author Affiliations
A Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, 108 N Greene St, Baltimore, MD 21201, USA.
B Corresponding author. Email: mnurminskaya@som.umaryland.edu
Reproduction, Fertility and Development 28(7) 974-981 https://doi.org/10.1071/RD14155
Submitted: 14 May 2014 Accepted: 14 November 2014 Published: 5 January 2015
Abstract
Use of the dietary supplement quercetin is on the rise. Because previous studies imply an inhibitory effect of quercetin on male fertility, we explored the effects of this flavonoid on fertility in female mice. Birth outcomes, and ovarian morphology in 4-week-old offspring, were assessed in mice receiving dietary quercetin (5 mg kg–1 day–1) for 9 months during two breeding periods: from 2 to 6 months (prime reproductive age) and 8 to11 months of age. Quercetin increased birth spacing, leading to a 60% reduction in the number of litters, but enhanced folliculogenesis in ovaries of female offspring. While in young females quercetin caused an almost 70% increase in litter size, in older animals this effect was reversed. Consistent with the inhibitory activity of quercetin on the enzyme transglutaminase 2 (TG2), genetic ablation of TG2 in mice mirrors the effects of quercetin on birth outcomes and follicular development. Further, TG2-null mice lack responsiveness to quercetin ingestion. Our study shows for the first time that dietary quercetin can cause reduced reproductive potential in female mice and implies that TG2 may regulate ovarian ageing.
Additional keywords: fecundity, folliculogenesis, litter size, offspring, ovary.
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