Role of linker histone H1c during the reprogramming of Chinese swamp buffalo (Bubalus Bubalis) embryos produced by somatic cell nuclear transfer
Gao-Bo Huang A B E , Li Quan A C E , Yong-Lian Zeng A B E , Jian Yang D , Ke-Huan Lu A C and Sheng-Sheng Lu A C F
+ Author Affiliations
- Author Affiliations
A State Key Laboratory for Conservation and Utilisation of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi 53004, China.
B College of Life Science and Technology, Guangxi University, Nanning, Guangxi 53004, China.
C College of Animal Science and Technology, Guangxi University, Nanning, Guangxi 53004, China.
D Tiandiyang Biotechnology Co. Ltd, Nanning, Guangxi 530004, China.
E These authors contributed equally to this work.
F Corresponding author. Email: shengshenglu@sina.com
Reproduction, Fertility and Development 28(3) 302-309 https://doi.org/10.1071/RD14051
Submitted: 13 February 2014 Accepted: 2 May 2014 Published: 22 August 2014
Abstract
During reprogramming, there is exchange of histone H1c and the oocyte-specific linker histone, and H1c may play a critically important role in the reprogramming process of somatic cell nuclear transfer (SCNT). The aim of the present study was to investigate the role of the H1c gene in SCNT reprogramming in Chinese swamp buffalo (Bubalus bubalis) using RNA interference (RNAi). Chinese swamp buffalo H1c gene sequences were obtained and H1c-RNAi vectors were designed, synthesised and then transfected into a buffalo fetal skin fibroblast cell line. Expression of H1c was determined by real-time polymerase chain reaction to examine the efficiency of vector interference. These cells were then used as a nuclear donor for SCNT so as to observe the further development of SCNT embryos. Inhibition of H1c gene expression in donor cells significantly improved the developmental speed of embryos from the 1-cell to 8-cell stage. Furthermore, compared with the control group, inhibition of H1c gene expression significantly reduced the blastocyst formation rate. It is concluded that linker histone H1c is very important in SCNT reprogramming in Chinese swamp buffalo. Correct expression of the H1c gene plays a significant role in preimplantation embryonic development in B. bubalis.
Additional keyword: RNA interference.
References
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