Metabolic profile of serum and follicular fluid from postpartum dairy cows during summer and winter
Benner G. Alves A C , Kele A. Alves A , Muller C. Martins B , Lucas S. Braga B , Thiago H. Silva B , Bruna G. Alves B , Ricarda M. Santos B , Thiago V. Silva A , Marco A. O. Viu A , Marcello E. Beletti B , José O. Jacomini B and Maria L. Gambarini A
+ Author Affiliations
- Author Affiliations
A Center for Studies and Research in Animal Reproductive Biology, College of Veterinary and Animal Science, Federal University of Goiás, Goiânia, GO, 74001-970, Brazil.
B Laboratory of Animal Reproduction, Faculty of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, MG, 38400-902, Brazil.
C Corresponding author. Email: bennervet@gmail.com
Reproduction, Fertility and Development 26(6) 866-874 https://doi.org/10.1071/RD13102
Submitted: 25 March 2013 Accepted: 4 June 2013 Published: 2 July 2013
Abstract
This study was designed to monitor the biochemical profiles of serum and follicular fluid (FF) of postpartum dairy cows during the summer (n = 30) and winter (n = 30). Blood and FF (follicles ≥ 9 mm) were obtained from Girolando cows at 30, 45, 60, 75 and 90 days postpartum. The samples were collected and analysed to determine glucose, total cholesterol (TC), triglyceride (TG), urea, sodium (Na), potassium (K) and calcium (Ca) levels. Throughout the study, the following clinical variables were measured: rectal temperature (RT), respiratory rate (RR) and body condition score (BCS). In addition, the temperature humidity index (THI) was calculated for each season. During the summer season, THI was higher, BCS decreased, there was an increase in RT, and glucose, urea, Na and K serum levels were decreased (P < 0.05). The levels of TC, TG, urea, K and Ca in follicular fluid increased (P < 0.05). Positive correlations (P < 0.05) were observed between the serum and FF levels for glucose (r = 0.29), TC (r = 0.24) and Ca (r = 0.30). Therefore, the biochemical profile of serum and FF of dairy cows under summer heat-stress conditions demonstrates marked changes that may impair fertility during lactation.
Additional keywords: electrolytes, heat stress.
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