Successful cloning of coyotes through interspecies somatic cell nuclear transfer using domestic dog oocytes
Insung Hwang A , Yeon Woo Jeong A , Joung Joo Kim A , Hyo Jeong Lee A , Mina Kang A , Kang Bae Park A , Jung Hwan Park A , Yeun Wook Kim A , Woo Tae Kim B , Taeyoung Shin A , Sang Hwan Hyun C , Eui-Bae Jeung C and Woo Suk Hwang A D
+ Author Affiliations
- Author Affiliations
A Sooam Biotech Research Foundation, 64 Kyungin-ro, Guro-gu, Seoul 152-895, Republic of Korea.
B Korea Gene Information Center, Inc., 201, A-command, 19-4, Yangpyeong-dong 2 ga, Yeongdeungpo-gu, Seoul 150-102, Republic of Korea.
C College of Veterinary Medicine, Chungbuk National University, 410 Sungbong-ro, Heungduk-gu, Cheongju, Chungbuk 361-763, Republic of Korea.
D Corresponding author. Email: hwangws@sooam.org
Reproduction, Fertility and Development 25(8) 1142-1148 https://doi.org/10.1071/RD12256
Submitted: 7 August 2012 Accepted: 23 October 2012 Published: 7 December 2012
Abstract
Interspecies somatic cell nuclear transfer (iSCNT) is an emerging assisted reproductive technology (ART) for preserving Nature’s diversity. The scarcity of oocytes from some species makes utilisation of readily available oocytes inevitable. In the present study, we describe the successful cloning of coyotes (Canis latrans) through iSCNT using oocytes from domestic dogs (Canis lupus familiaris or dingo). Transfer of 320 interspecies-reconstructed embryos into 22 domestic dog recipients resulted in six pregnancies, from which eight viable offspring were delivered. Fusion rate and cloning efficiency during iSCNT cloning of coyotes were not significantly different from those observed during intraspecies cloning of domestic dogs. Using neonatal fibroblasts as donor cells significantly improved the cloning efficiency compared with cloning using adult fibroblast donor cells (P < 0.05). The use of domestic dog oocytes in the cloning of coyotes in the present study holds promise for cloning other endangered species in the Canidae family using similar techniques. However, there are still limitations of the iSCNT technology, as demonstrated by births of morphologically abnormal coyotes and the clones’ inheritance of maternal domestic dog mitochondrial DNA.
Additional keywords: assisted reproductive technology, cloning efficiency, donor cell type, species conservation.
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