Role of Fyn kinase in oocyte developmental potential
Jinping Luo A , Lynda K. McGinnis A and William H. Kinsey A B
+ Author Affiliations
- Author Affiliations
A Center for Reproductive Sciences, University of Kansas Medical Center, Kansas City, KS 66160, USA.
B Corresponding author. Email: wkinsey@kumc.edu
Reproduction, Fertility and Development 22(6) 966-976 https://doi.org/10.1071/RD09311
Submitted: 23 December 2009 Accepted: 20 January 2010 Published: 1 July 2010
Abstract
Fyn kinase is highly expressed in oocytes, with inhibitor and dominant-negative studies suggesting a role in the signal transduction events during egg activation. The purpose of the present investigation was to test the hypothesis that Fyn is required for calcium signalling, meiosis resumption and pronuclear congression using the Fyn-knockout mouse as a model. Accelerated breeding studies revealed that Fyn-null females produced smaller litter sizes at longer intervals and exhibited a rapid decline in pup production with increasing age. Fyn-null females produced a similar number of oocytes, but the frequency of immature oocytes and mature oocytes with spindle chromosome abnormalities was significantly higher than in controls. Fertilised Fyn-null oocytes frequently (24%) failed to undergo pronuclear congression and remained at the one-cell stage. Stimulation with gonadotropins increased the number of oocytes ovulated, but did not overcome the above defects. Fyn-null oocytes overexpressed Yes kinase in an apparent effort to compensate for the loss of Fyn, yet still exhibited an altered pattern of protein tyrosine phosphorylation. In summary, Fyn-null female mice exhibit reduced fertility that appears to result from actin cytoskeletal defects rather than calcium signalling. These defects cause developmental arrest during oocyte maturation and pronuclear congression.
Additional keywords: actin, cytoskeleton, fertility, mouse, protein kinase, spindle.
Acknowledgements
The authors are indebted to Lily Zhang for technical assistance. This work was supported by grants from the National Institute of Child Health and Human Development (USA) (14846; to W.H.K.) and the National Center For Research Resources (P20RR024214).
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