Leech breach: a first record of the invasive freshwater leech Helobdella europaea (Hirudinea: Glossiphoniidae) in Fiji
Bindiya Rashni
A , Kelly T. Brown B , Patricia A. McLenachan C , Peter J. Lockhart C , Paul C. Southgate D and Monal M. Lal B D *
A Institute of Applied Science, School of Agriculture, Geography, Environment, Ocean and Natural Sciences, University of the South Pacific, Lower Laucala Campus, Laucala Bay Road, Suva, Fiji.
B Discipline of Marine Studies, School of Agriculture, Geography, Environment, Ocean and Natural Sciences, University of the South Pacific, Lower Laucala Campus, Laucala Bay Road, Suva, Fiji.
C School of Natural Sciences, College of Sciences, Massey University, Palmerston North 4442, New Zealand.
D Australian Centre for Pacific Islands Research and School of Science, Technology and Engineering, University of the Sunshine Coast, Maroochydore, Qld 4558, Australia.
Abstract
The freshwater flat leech Helobdella europaea Kutschera, 1987 is a small annelid indigenous to South America. This invasive species feeds on the haemolymph of host aquatic invertebrates, with occurrences reported from Europe, USA, Taiwan, North Africa, Hawai‘i, Australia and New Zealand. A large number of individuals were discovered in the Ba River catchment, Fiji, during a 2015–2020 freshwater biodiversity survey, raising concerns of potential impacts on endemic Fijian aquatic invertebrate fauna and ecosystem integrity.
To facilitate assessments of its spread and ethology, this study employed morphological and phylogenetic analyses for verification of taxonomic identity.
Phylogenetic trees were constructed using a 658 bp fragment of the mitochondrial DNA cox1 (COI) gene. The first complete mitochondrial genome sequence of H. europaea was also determined using selective multiple displacement amplification and Oxford Nanopore Technology to provide a reference for future comparative analyses and source tracking of spread to other regions.
Morphological and COI analyses identified all Fijian leech specimens collected (n = 16) as H. europaea, reporting the first occurrence of this species on a south-west Pacific Island. The complete mitochondrial genome was sequenced.
Confirmation of its presence in Fiji is a national biosecurity concern and will guide the Biosecurity Authority of Fiji and national agencies in further ecosystem assessment and response strategies.
With the complete mitochondrial genome of H. europaea now available, transmission pathway traceability is possible in other regions where this species may be detected.
Keywords: biosecurity, DNA barcoding, freshwater, invasive, leech, mitochondrial DNA, taxonomic identification, traceability.
References
Adamkewicz SL, Harasewych MG (1996) Systematics and biogeography of the genus Donax (Bivalvia: Donacidae) in eastern North America. American Malacological Bulletin 13, 97-103.
| Google Scholar |
Bely AE, Weisblat DA (2006) Lessons from leeches: a call for DNA barcoding in the lab. Evolution & Development 8, 491-501.
| Crossref | Google Scholar |
Carew ME, Coleman RA, Hoffmann AA (2018) Can non-destructive DNA extraction of bulk invertebrate samples be used for metabarcoding? PeerJ 6, e4980.
| Crossref | Google Scholar |
Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32, 1792-1797.
| Crossref | Google Scholar |
Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit i from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294-299.
| Google Scholar |
GBIF (2020) GBIF occurrence download. Global Biodiversity Information Facility. Available at https://doi.Org/10.15468/dl.Anfxjg[Accessed 17 August 2020]
Govedich FR, Davies RW (1998) The first record of the genus Helobdella (Hirudinoidea: Glossiphoniidae) from Australia, with a description of a new species, Helobdella papillornata. Hydrobiologia 389, 45-49.
| Crossref | Google Scholar |
Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17, 754-755.
| Crossref | Google Scholar |
Huson DH, Richter DC, Rausch C, Dezulian T, Franz M, Rupp R (2007) Dendroscope: an interactive viewer for large phylogenetic trees. BMC Bioinformatics 8, 460.
| Crossref | Google Scholar |
Jiménez-Armenta J, Oceguera-Figueroa A (2019) Leeches from Mexico City, remnants of the ancient lake. Mitochondrial DNA Part A 30, 632-642.
| Crossref | Google Scholar |
Jueg U (2008) Alboglossiphonia iberica nov. sp. – eine neue Egelart von der Iberischen halbinsel (Hirudinea: Glossiphoniidae). Lauterbornia 65, 43-61.
| Google Scholar |
Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28, 1647-1649.
| Crossref | Google Scholar |
Kutschera U (1985) Beschreibung einer neuen egelart, Helobdella striata nov. sp. (Hirudinea: Glossiphoniidae). Zoologische Jahrbücher. Abteilung für Systematik, Ökologie und Geographie der Tiere 112, 469-476.
| Google Scholar |
Kutschera U (1987) Notes on the taxonomy and biology of leeches of the genus Helobdella Blanchard 1896 (Hirudinea: Glossiphoniidae). Zoologischer Anzeiger 219, 321-323.
| Google Scholar |
Kutschera U (1989) Reproductive behaviour and parental care of the leech Helobdella californica (Hirudinea: Glossiphoniidae). Zoologischer Anzeiger 222, 122-128.
| Google Scholar |
Kutschera U (2004) The freshwater leech Helobdella europaea (Hirudinea: Glossiphoniidae): an invasive species from South America? Lauterbornia 52, 153-162.
| Google Scholar |
Kutschera U, Langguth H, Kuo D-H, Weisblat DA, Shankland M (2013) Description of a new leech species from North America, Helobdella austinensis n. sp. (hirudinea: Glossiphoniidae), with observations on its feeding behaviour. Zoosystematics and Evolution 89, 239-246.
| Crossref | Google Scholar |
Lai Y-T, Chang C-H, Chen J-H (2009) Two new species of Helobdella Blanchard 1896 (Hirudinida: Rhynchobdellida: Glossiphoniidae) from Taiwan, with a checklist of hirudinea fauna of the island. Zootaxa 2068, 27-46.
| Crossref | Google Scholar |
Lal MM, Southgate PC, Jerry DR, Zenger KR (2016) Fishing for divergence in a sea of connectivity: the utility of ddRADseq genotyping in a marine invertebrate, the black-lip pearl oyster Pinctada margaritifera. Marine Genomics 25, 57-68.
| Crossref | Google Scholar |
Mabrouki Y, Ahmed RB, Taybi AF, Rueda J (2019) An annotated checklist of the leech (Annelida: Hirudinida) species of the Moulouya River basin, Morocco, with several new distribution records and a historical overview. African Zoology 54, 199-214.
| Crossref | Google Scholar |
Mãgalhaes WF, Hutchings P, Oceguera-Figueroa A, Martin P, Schmelz RM, Wetzel MJ, Wiklund H, Maciolek NJ, Kawauchi GY, Williams JD (2021) Segmented worms (Phylum Annelida): a celebration of twenty years of progress through Zootaxa and call for action on the taxonomic work that remains. Zootaxa 4979, 190-211.
| Crossref | Google Scholar |
Málnás K, Kovács K, Ficsór M, Juhász P, Müller Z (2016) Appearances of the non-indigenous Helobdella europaea Kutschera, 1987 (Hirudinea, Glossiphoniidae) in Hungarian watercourses. Folia Historico-Naturalia Musei Matraensis 40, 17-20.
| Google Scholar |
Morhun H, Sidorovskyi S, Khomenko A, Mazepa G, Utevsky S (2021) First Ukrainian record of the invasive leech Helobdella europaea (Hirudinea: Glossiphoniidae) from an aquarium in Kharkiv: morphological variability and phylogenetic relationships. Biologia 76, 193-202.
| Crossref | Google Scholar |
Munwes I, Geffen E, Friedmann A, Tikochinski Y, Gafny S (2011) Variation in repeat length and heteroplasmy of the mitochondrial DNA control region along a core–edge gradient in the eastern spadefoot toad (Pelobates syriacus). Molecular Ecology 20, 2878-2887.
| Crossref | Google Scholar |
Perera A, Hernandez-Sastre P, Ayres C (2019) Hitch me a ride: first report of the alien leech Helobdella octatestisaca in Europe associated with freshwater turtles. Biological Invasions 21, 3467-3471.
| Crossref | Google Scholar |
Pfeiffer I, Brenig B, Kutschera U (2004) The occurrence of an Australian leech species (genus Helobdella) in German freshwater habitats as revealed by mitochondrial DNA sequences. Molecular Phylogenetics and Evolution 33, 214-219.
| Crossref | Google Scholar |
Posada D (2008) jModelTest: phylogenetic model averaging. Molecular Biology and Evolution 25, 1253-1256.
| Crossref | Google Scholar |
Rambaut A (2014) Figtree v.1.4.2: tree drawing tool. Available at https://github.com/rambaut/figtree/releases
Rambaut A, Suchard M, Drummond AJ (2003) Tracer (v.1.6.). Available at https://github.com/beast-dev/tracer/releases
Rashni B (2014b) Chapter 6: Freshwater macroinvertebrates. In: Pene S, Tuiwawa M, FPAM (2014) Biological and Socio-economical baseline report for the establishment of the greater Delaikoro protected area, Vanua Levu, Fiji Islands. A rapid biodiversity assessment, socioeconomic study and archaeological survey of the greater Delaikoro area, June 2014, Suva, Fiji, FPAM-2014-Biodiversity-01. Institute of Applied Science. pp. 53–72.
Rashni B (2020) Freshwater invertebrate assemblages and ecological status of the Ba River, Fiji. In ‘First series technical consultation of the regional scientific and technical committee for the GEF Pacific Ridge to Reef programme. Nadi, Fiji’, p. 27. GEF Pacific Ridge to Reef Programme. Available at https://www.pacific-r2r.org/sites/default/files/2020-05/First_Series_Technical_Consultation_of_the_RSTC.pdf
Rashni B, Brown KT (2021) Hellobdella europaea occurrence in Fiji. Occurrence dataset. The University of the South Pacific: GBIF. Available at https://doi.org/10.15468/c2yhjc
Reyes-Prieto M, Oceguera-Figueroa A, Snell S, Negredo A, Barba E, Fernández L, Moya A, Latorre A (2014) DNA barcodes reveal the presence of the introduced freshwater leech Helobdella europaea in Spain. Mitochondrial DNA 25, 387-393.
| Crossref | Google Scholar |
Richardson DJ, Moser WE, Hammond CI, Lazo-Wasem EA (2015) New host and geographic distribution records for Glossiphoniid leeches associated with turtles in southern New England. Comparative Parasitology 82, 240-243.
| Crossref | Google Scholar |
Richardson DJ, Moser WE, Hammond CI, Lazo-Wasem EA, McAllister CT, Pulis EE (2017) A new species of leech of the genus Placobdella (Hirudinida, Glossiphoniidae) from the American alligator (Alligator mississippiensis) in Mississippi, USA. ZooKeys 667, 39-49.
| Crossref | Google Scholar |
Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572-1574.
| Crossref | Google Scholar |
Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61, 539-542.
| Crossref | Google Scholar |
Schmerer MW, Null RW, Shankland M (2013) Developmental transition to bilaterally symmetric cell divisions is regulated by Pax-mediated transcription in embryos of the leech Helobdella austinensis. Developmental Biology 382, 149-159.
| Crossref | Google Scholar |
Seaver EC (2003) Segmentation: mono- or polyphyletic? International Journal of Developmental Biology 47, 583-595.
| Google Scholar |
Siddall ME, Borda E (2003) Phylogeny and revision of the leech genus Helobdella (Glossiphoniidae) based on mitochondrial gene sequences and morphological data and a special consideration of the triserialis complex. Zoologica Scripta 32, 23-33.
| Crossref | Google Scholar |
Siddall ME, Budinoff RB (2005) DNA-barcoding evidence for widespread introductions of a leech from the South American Helobdella triserialis complex. Conservation Genetics 6, 467-472.
| Crossref | Google Scholar |
Siddall ME, Burreson EM (1998) Phylogeny of leeches (Hirudinea) based on mitochondrial cytochrome c oxidase subunit I. Molecular Phylogenetics and Evolution 9, 156-162.
| Crossref | Google Scholar |
Sket B, Trontelj P (2008) Global diversity of leeches (Hirudinea) in freshwater. Hydrobiologia 595, 129-137.
| Crossref | Google Scholar |
Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10, 512-526.
| Crossref | Google Scholar |
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30, 2725-2729.
| Crossref | Google Scholar |
Tessler M, Siddall ME, Oceguera-Figueroa A (2018) Leeches from Chiapas, Mexico, with a new species of Erpobdella (Hirudinida: Erpobdellidae). American Museum Novitates 3895, 1-15.
| Crossref | Google Scholar |
van Haaren T, Hop P, Soes M, Tempelman D (2004) The freshwater leeches (Hirudinea) of the Netherlands. Lauterbornia 52, 113-131.
| Google Scholar |
Weisblat DA, Huang FZ (2001) An overview of glossiphoniid leech development. Canadian Journal of Zoology 79, 218-232.
| Crossref | Google Scholar |
Wick RR (2018) Porechop. Available at https://github.com/rrwick/Porechop
Wick RR, Judd LM, Cerdeira LT, Hawkey J, Méric G, Vezina B, Wyres KL, Holt KE (2021) Trycycler: consensus long-read assemblies for bacterial genomes. Genome Biology 22, 266.
| Crossref | Google Scholar |
Zielske S, Haase M (2014) New insights into tateid gastropods and their radiation on Fiji based on anatomical and molecular methods (Caenogastropoda: Truncatelloidea). Zoological Journal of the Linnean Society 172, 71-102.
| Crossref | Google Scholar |
