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Advances in the aquatic sciences
RESEARCH ARTICLE

Abundance, distribution and reproduction of the Data-Deficient species (Squalus blainville) around Sardinia Island (central western Mediterranean Sea) as a contribution to its conservation

Martina Francesca Marongiu https://orcid.org/0000-0002-0582-8865 A B D , Cristina Porcu https://orcid.org/0000-0003-2649-6502 A B , Andrea Bellodi https://orcid.org/0000-0002-9017-1692 A B , Rita Cannas https://orcid.org/0000-0001-6627-4053 A B , Pierluigi Carbonara https://orcid.org/0000-0002-2529-2535 C , Alessandro Cau https://orcid.org/0000-0003-4082-7531 A B , Elisabetta Coluccia https://orcid.org/0000-0003-4412-8033 A , Davide Moccia https://orcid.org/0000-0001-7306-8573 A B , Antonello Mulas https://orcid.org/0000-0003-1635-691X A B , Paola Pesci https://orcid.org/0000-0002-9066-8076 A B and Maria Cristina Follesa https://orcid.org/0000-0001-8320-9974 A B
+ Author Affiliations
- Author Affiliations

A Dipartimento di Scienze della Vita e dell’Ambiente, Università degli Studi di Cagliari, Via Tommaso Fiorelli 1, I-09126 Cagliari, Italy.

B Consorzio Nazionale Interuniversitario per le Scienze Mare (CoNISMa), Piazzale Flaminio 9, I-00196 Roma, Italy.

C COISPA Tecnologia & Ricerca – Stazione Sperimentale per lo Studio delle Risorse del Mare, Via dei Trulli 18/20, I-70126 Bari, Italy.

D Corresponding author. Email: mfmarongiu@unica.it

Marine and Freshwater Research 72(1) 118-130 https://doi.org/10.1071/MF19372
Submitted: 28 November 2019  Accepted: 19 March 2020   Published: 13 May 2020

Abstract

Data-Deficient (DD) species are currently under threat because of the paucity of information regarding their abundance, distribution and biological traits. In this study we analysed temporal trends and bioecological features of the DD shark, Squalus blainville. Biological and fishery data were collected through two different scientific surveys spanning 1994–2018 and commercial hauls around Sardinia Island (central western Mediterranean) from 2008 to 2018. The analysis of temporal trends based on abundance data revealed a significant population decline over time. The late attainment of maturity and low fecundity of S. blainville are suspected to have contributed to making it highly vulnerable to overfishing. No bathymetric segregation by sex and size was found. However, the almost exclusive presence of pregnant females south-west of Sardinia could be due to sexual spatial segregation. This is also supported by histological findings showing long-term sperm storage to ensure the persistence of the species when females and males are separated. The documented decline of this species, its low reproductive potential and similarities in the depth distribution of immature and mature individuals indicate the need for urgent protection plans for S. blainville in Sardinian waters.

Additional keywords: histology, longnose spurdog, sexual spatial segregation, sperm storage.


References

Adams, S., Mapstone, B. D., Russ, G. R., and Davies, C. R. (2000). Geographic variation in the sex ratio, sex specific size, and age structure of Plectropomus leopardus (Serranidae) between reefs open and closed to fishing on the Great Barrier Reef. Canadian Journal of Fisheries and Aquatic Sciences 57, 1448–1458.
Geographic variation in the sex ratio, sex specific size, and age structure of Plectropomus leopardus (Serranidae) between reefs open and closed to fishing on the Great Barrier Reef.Crossref | GoogleScholarGoogle Scholar |

Anastasopoulou, A., Mytilineou, C., Makantasi, P., Smith, C. J., Kavadas, S., Lefkaditou, E., and Papadopoulou, K. N. (2018). Life history aspects of two species of the Squalus genus in the eastern Ionian Sea. Journal of the Marine Biological Association of the United Kingdom 98, 937–948.
Life history aspects of two species of the Squalus genus in the eastern Ionian Sea.Crossref | GoogleScholarGoogle Scholar |

Ashe, E., Noren, D. P., and Williams, R. (2010). Animal behaviour and marine protected areas: incorporating behavioural data into the selection of marine protected areas for an endangered killer whale population. Animal Conservation 13, 196–203.
Animal behaviour and marine protected areas: incorporating behavioural data into the selection of marine protected areas for an endangered killer whale population.Crossref | GoogleScholarGoogle Scholar |

Awruch, C. A., Frusher, S. D., Pankhurst, N. W., and Stevens, J. D. (2008). Non-lethal assessment of reproductive characteristics for management and conservation of sharks. Marine Ecology Progress Series 355, 277–285.
Non-lethal assessment of reproductive characteristics for management and conservation of sharks.Crossref | GoogleScholarGoogle Scholar |

Awruch, C. A., Jones, S. M., García Asorey, M., and Barnett, A. (2014). Non-lethal assessment of the reproductive status of sevengill sharks Notorynchus cepedianus to determine the significance of habitat use in coastal areas. Conservation Physiology 2, cou013.
Non-lethal assessment of the reproductive status of sevengill sharks Notorynchus cepedianus to determine the significance of habitat use in coastal areas.Crossref | GoogleScholarGoogle Scholar | 27293634PubMed |

Bargione, G., Donato, F., La Mesa, M., Mazzoldi, C., Riginella, E., Vasapollo, C., Virgili, M., and Lucchetti, A. (2019). Life-history traits of the spiny dogfish Squalus acanthias in the Adriatic Sea. Scientific Reports 9, 14317.
Life-history traits of the spiny dogfish Squalus acanthias in the Adriatic Sea.Crossref | GoogleScholarGoogle Scholar | 31586144PubMed |

Barnett, A., Braccini, J. M., Awruch, C. A., and Ebert, D. A. (2012). An overview on the role of Hexanchiformes in marine ecosystems: biology, ecology and conservation status of a primitive order of modern sharks. Journal of Fish Biology 80, 966–990.
An overview on the role of Hexanchiformes in marine ecosystems: biology, ecology and conservation status of a primitive order of modern sharks.Crossref | GoogleScholarGoogle Scholar | 22497370PubMed |

Baum, J. K., Myers, R. A., Kehler, D. G., Worm, B., Harley, S. J., and Doherty, P. A. (2003). Collapse and conservation of shark populations in the northwest Atlantic. Science 299, 389–392.
Collapse and conservation of shark populations in the northwest Atlantic.Crossref | GoogleScholarGoogle Scholar | 12532016PubMed |

Bellodi, A., Porcu, C., Cannas, R., Cau, A., Marongiu, M. F., Mulas, A., Vittori, S., and Follesa, M. C. (2017). Life-history traits of the long-nosed skate Dipturus oxyrinchus. Journal of Fish Biology 90, 867–888.
Life-history traits of the long-nosed skate Dipturus oxyrinchus.Crossref | GoogleScholarGoogle Scholar | 27873321PubMed |

Bellodi, A., Porcu, C., Cau, A., Marongiu, M. F., Melis, R., Mulas, A., Pesci, P., Follesa, M. C., and Cannas, R. (2018). Investigation on the genus Squalus in the Sardinian waters (central-western Mediterranean) with implications on its management. Mediterranean Marine Science 19, 256–272.
Investigation on the genus Squalus in the Sardinian waters (central-western Mediterranean) with implications on its management.Crossref | GoogleScholarGoogle Scholar |

Bonello, J., Bonnici, L., Ferrari, A., Cariani, A., and Schembri, P. (2016). Not all that clear cut: intraspecific morphological variability in Squalus blainville (Risso, 1827) and implications for identification of the species. Journal of the Marine Biological Association of the United Kingdom 96, 1585–1596.
Not all that clear cut: intraspecific morphological variability in Squalus blainville (Risso, 1827) and implications for identification of the species.Crossref | GoogleScholarGoogle Scholar |

Bonnici, L., Bonello, J. J., and Schembri, P. J. (2018). Diet and trophic level of the longnose spurdog, Squalus blainville (Risso, 1826) in the 25-nautical mile Fisheries Management Zone around the Maltese Islands. Regional Studies in Marine Science 19, 33–42.
Diet and trophic level of the longnose spurdog, Squalus blainville (Risso, 1826) in the 25-nautical mile Fisheries Management Zone around the Maltese Islands.Crossref | GoogleScholarGoogle Scholar |

Brooks, E. J., Sloma, K. A., Sims, D. W., and Danylchuk, A. J. (2011). Validating the use of baited remote underwater video surveys for assessing the diversity, distribution and abundance of sharks in the Bahamas. Endangered Species Research 13, 231–243.
Validating the use of baited remote underwater video surveys for assessing the diversity, distribution and abundance of sharks in the Bahamas.Crossref | GoogleScholarGoogle Scholar |

Cannizzaro, L., Rizzo, P., Levi, D., and Gancitano, S. (1995). Age determination and growth of Squalus blainvillei (Risso, 1826). Fisheries Research 23, 113–125.
Age determination and growth of Squalus blainvillei (Risso, 1826).Crossref | GoogleScholarGoogle Scholar |

Capapé, C. (1975). Etude du régime alimantaire de Squalus blainville (Risso, 1826) des côte tunisiennes. Bulletin de l’Institut National Scientifique et Technique d’Oceanographie et de Peche, Salammbô 4, 61–73.

Capapé, C. (1983). Nouvelles données sur la biologie de la reproduction de Mustelus asterias Cloquet, 1821 (Pisces, Pleurotremata, Triakidae) des côtes Tunisiennes. Vie et Milieu 33, 143–152.

Castro, J. I. (2009). Observations on the reproductive cycles of some viviparous North American sharks. Aqua: International Journal of Ichthyology 15, 205–222.

Cau, A. (2008). Valutazione Risorse Demersali – Gruppo Nazionali Demersali – G.R.U.N.D. – GSA 11 – Mari circostanti la Sardegna. Relazione finale. Ministero delle Politiche Agricole Alimentari e Forestali, Rome, Italy.

Cau, A., Follesa, M. C., Moccia, D., Bellodi, A., Mulas, A., Bo, M., Canese, S., Angiolillo, M., and Cannas, R. (2017). Leiopathes glaberrima millennial forest from SW Sardinia as nursery ground for the small spotted catshark Scyliorhinus canicula. Aquatic Conservation 27, 731–735.
Leiopathes glaberrima millennial forest from SW Sardinia as nursery ground for the small spotted catshark Scyliorhinus canicula.Crossref | GoogleScholarGoogle Scholar |

Cerri, P. S., and Sasso-Cerri, E. (2003). Staining methods applied to glycol methacrylate embedded tissue sections. Micron 34, 365–372.
Staining methods applied to glycol methacrylate embedded tissue sections.Crossref | GoogleScholarGoogle Scholar | 14680922PubMed |

Clarke, M. W., Connolly, P. L., and Bracken, J. J. (2001). Aspects of reproduction of the deep-water sharks Centroscymnus coelolepis and Centrophorus squamosus from west of Ireland and Scotland. Journal of the Marine Biological Association of the United Kingdom 81, 1019–1029.
Aspects of reproduction of the deep-water sharks Centroscymnus coelolepis and Centrophorus squamosus from west of Ireland and Scotland.Crossref | GoogleScholarGoogle Scholar |

Clarke, M. W., Connolly, P. L., and Bracken, J. J. (2002). Catch, discarding, age estimation and maturity of the squalid shark Deania calceus west and north of Ireland. Fisheries Research 56, 139–153.
Catch, discarding, age estimation and maturity of the squalid shark Deania calceus west and north of Ireland.Crossref | GoogleScholarGoogle Scholar |

Colloca, F., Garofalo, G., Bitetto, I., Facchini, M. T., Grati, F., Martiradonna, A., Mastrantonio, G., Nikolioudakis, N., Ordinas, F., Scarcella, G., Tserpes, G., Pilar Tugores, M., Valavanis, V., Carlucci, R., Fiorentino, F., Follesa, M. C., Iglesias, M., Knittweis, L., Lefkaditou, E., Lembo, G., Manfredi, C., Massuti, E., Pace, M. L., Papadopoulou, N., Sartor, P., Smith, C. J., and Spedicato, M. T. (2015). The seascape of demersal fish nursery areas in the north Mediterranean Sea, a first step towards the implementation of spatial planning for trawl fisheries. PLoS One 10, e0119590.
The seascape of demersal fish nursery areas in the north Mediterranean Sea, a first step towards the implementation of spatial planning for trawl fisheries.Crossref | GoogleScholarGoogle Scholar | 25785737PubMed |

Colton, M. A., and Swearer, S. E. (2010). A comparison of two survey methods: differences between underwater visual census and baited remote underwater video. Marine Ecology Progress Series 400, 19–36.
A comparison of two survey methods: differences between underwater visual census and baited remote underwater video.Crossref | GoogleScholarGoogle Scholar |

Conrath, C. L. (2005). Reproductive biology. In ‘Management Techniques for Elasmobranchs Fisheries’. (Eds J. A. Musick and R. Bonfil.) FAO Fishery technical paper number 474, pp. 103–126. (Food and Agriculture Organization of the United Nations: Rome, Italy.)

Cortés, E. (2002). Stock assessment of small coastal sharks in the US Atlantic and Gulf of Mexico. Sustainable Fisheries Division Contribution SFD-01/02-152. Available at http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.569.972&rep=rep1&type=pdf [Verified 3 April 2020].

Dulvy, N. K., Allen, D. J., Ralph, G. M., and Walls, R. H. L. (2016). The conservation status of sharks, rays and chimaeras in the Mediterranean Sea. [Brochure] (IUCN: Malaga, Spain.) Available at https://www.iucn.org/sites/dev/files/content/documents/brochure_medredlist_sharks.pdf [Verified 3 April 2020].

Ebert, D. A., Cowley, P. D., and Compagno, L. J. V. (2002). First records of the longnose spiny dogfish Squalus blainvillei (Squalidae) and the deep-water stingray Plesiobatis daviesi (Urolophidae) from South African waters. South African Journal of Marine Science 24, 355–357.
First records of the longnose spiny dogfish Squalus blainvillei (Squalidae) and the deep-water stingray Plesiobatis daviesi (Urolophidae) from South African waters.Crossref | GoogleScholarGoogle Scholar |

Ebert, D. A., Compagno, L. J. V., and Cowley, P. D. (2008). Aspects of the reproductive biology of skates (Chondrichthyes: Rajiformes: Rajoidei) from southern Africa. ICES Journal of Marine Science 65, 81–102.
Aspects of the reproductive biology of skates (Chondrichthyes: Rajiformes: Rajoidei) from southern Africa.Crossref | GoogleScholarGoogle Scholar |

Ebert, D. A., Serena, F., and Mancusi, C. (2009). Longnose spurdog Squalus blainville. In ‘The IUCN Red List of Threatened Species 2009’. e.T161536A5446109. (International Union for Conservation of Nature and Natural Resources.) Available at https://www.iucnredlist.org/species/161536/5446109 [Verified 18 February 2019].

Ebert, D. A., Fowler, S. L., and Compagno, L. J. V. (Eds) (2013). ‘Sharks of the World – a Fully Illustrated Guide.’ (Wild Nature Press: Plymouth, UK.)

Economakis, A. E., and Lobel, P. S. (1998). Aggregation behavior of the grey reef shark, Carcharhinus amblyrhynchos, at Johnston Atoll, central Pacific Ocean. Environmental Biology of Fishes 51, 129–139.
Aggregation behavior of the grey reef shark, Carcharhinus amblyrhynchos, at Johnston Atoll, central Pacific Ocean.Crossref | GoogleScholarGoogle Scholar |

Finucci, B., Dunn, M. R., and Jones, E. G. (2018). Aggregations and associations in deep-sea chondrichthyans. ICES Journal of Marine Science 75, 1613–1626.
Aggregations and associations in deep-sea chondrichthyans.Crossref | GoogleScholarGoogle Scholar |

Follesa, M. C., and Carbonara, P. (2019). Atlas of the maturity stages of Mediterranean fishery resources. Studies and Reviews number 99, Food and Agriculture Organization of the United Nations, Rome, Italy.

Follesa, M. C., Marongiu, M. F., Zupa, W., Bellodi, A., Cau, A., Cannas, R., Colloca, F., Djurovic, M., Isajlovic, I., Jadaud, A., Manfredi, C., Mulas, A., Peristeraki, P., Porcu, C., Ramirez-Amaro, S., Salmerón Jiménez, F., Serena, F., Sion, L., Thasitis, I., Cau, A., and Carbonara, P. (2019a). Spatial variability of Chondrichthyes in the northern Mediterranean. Scientia Marina 83, 81–100.
Spatial variability of Chondrichthyes in the northern Mediterranean.Crossref | GoogleScholarGoogle Scholar |

Follesa, M. C., Agus, B., Bellodi, A., Cannas, R., Capezzuto, F., Casciaro, L., Cau, A., Cuccu, D., Donnaloia, M., Fernandez-Arcaya, U., Gancitano, V., Gaudio, P., Marongiu, M. F., Mulas, A., Pesci, P., Porcu, C., Rossetti, I., Sion, L., Vallisneri, M., and Carbonara, P. (2019b). The MEDITS maturity scales as a useful tool for investigating the reproductive traits of key species in the Mediterranean Sea. Scientia Marina 83, 235–256.
The MEDITS maturity scales as a useful tool for investigating the reproductive traits of key species in the Mediterranean Sea.Crossref | GoogleScholarGoogle Scholar |

Franklin, J. (Ed.) (2009). ‘Mapping Species Distributions: Spatial Inference and Prediction.’ (Cambridge University Press: Cambridge, UK.)

Girard, M., and Du Buit, M. H. (1999). Reproductive biology of two deep-water sharks from the British Isles, Centroscymnus coelolepis and Centrophorus squamosus. Journal of the Marine Biological Association of the United Kingdom 79, 923–931.
Reproductive biology of two deep-water sharks from the British Isles, Centroscymnus coelolepis and Centrophorus squamosus.Crossref | GoogleScholarGoogle Scholar |

Henderson, A. C., and Casey, Á. (2001). Reproduction and growth in the lesser-spotted dogfish Scyliorhinus canicula (Elasmobranchii; Scyliorhinidae), from the west coast of Ireland. Cahiers de Biologie Marine 42, 397–405.

Henderson, A. C., Flannery, K., and Dunne, J. (2002). Growth and reproduction in spiny dogfish Squalus acanthias L. (Pisces: Elasmobranchii), from the west coast of Ireland. Sarsia 87, 350–361.
Growth and reproduction in spiny dogfish Squalus acanthias L. (Pisces: Elasmobranchii), from the west coast of Ireland.Crossref | GoogleScholarGoogle Scholar |

International Council for the Exploration of the Sea (2008). Report of the Workshop on Maturity Ogive Estimation for Stock Assessment (WKMOG), 3–6 June 2008, Lisbon, Portugal. ICES CM2008/ACOM: 33, Copenhagen, Denmark.

International Council for the Exploration of the Sea (2018). Report of the Workshop for Advancing Sexual Maturity Staging in Fish (WKASMSF), 30 April–4 May 2018, ICES Headquarters, Copenhagen, Denmark. ICES CM/EOSG: 38, Copenhagen, Denmark.

King, M. (1995). ‘Fisheries Biology, Assessment and Management.’ (Fishing New Books: Oxford, UK.)

Klimley, P. A. (1987). The determinants of sexual segregation in the scalloped hammerhead shark, Sphyrna lewini. Environmental Biology of Fishes 18, 27–40.
The determinants of sexual segregation in the scalloped hammerhead shark, Sphyrna lewini.Crossref | GoogleScholarGoogle Scholar |

Kousteni, V., and Megalofonou, P. (2011). Reproductive biology and embryonic development of Squalus blainvillei in the eastern Mediterranean Sea. Scientia Marina 75, 237–249.
Reproductive biology and embryonic development of Squalus blainvillei in the eastern Mediterranean Sea.Crossref | GoogleScholarGoogle Scholar |

Kousteni, V., and Megalofonou, P. (2015). Aging and life history traits of the longnose spiny dogfish in the Mediterranean Sea: new insights into conservation and management needs. Fisheries Research 168, 6–19.
Aging and life history traits of the longnose spiny dogfish in the Mediterranean Sea: new insights into conservation and management needs.Crossref | GoogleScholarGoogle Scholar |

Kousteni, V., Kasapidis, P., Kotoulas, G., and Megalofonou, P. (2016). Evidence of high genetic connectivity for the longnose spurdog Squalus blainville in the Mediterranean Sea. Mediterranean Marine Science 17, 371–383.
Evidence of high genetic connectivity for the longnose spurdog Squalus blainville in the Mediterranean Sea.Crossref | GoogleScholarGoogle Scholar |

Kousteni, V., Karachle, P. K., and Megalofonou, P. (2017). Diet and trophic level of the longnose spurdog Squalus blainville (Risso, 1826) in the deep waters of the Aegean Sea. Deep-sea Research – I. Oceanographic Research Papers 124, 93–102.
Diet and trophic level of the longnose spurdog Squalus blainville (Risso, 1826) in the deep waters of the Aegean Sea.Crossref | GoogleScholarGoogle Scholar |

Marongiu, M. F., Porcu, C., Bellodi, A., Cuccu, D., Mulas, A., and Follesa, M. C. (2015). Oviducal gland microstructure of Raja miraletus and Dipturus oxyrinchus (Elasmobranchii, Rajidae). Journal of Morphology 276, 1392–1403.
Oviducal gland microstructure of Raja miraletus and Dipturus oxyrinchus (Elasmobranchii, Rajidae).Crossref | GoogleScholarGoogle Scholar | 26474106PubMed |

Marongiu, M. F., Porcu, C., Bellodi, A., Cannas, R., Cau, Al., Cuccu, D., Mulas, A., and Follesa, M. C. (2017). Temporal dynamics of demersal chondrichthyan species in the central western Mediterranean Sea: The case study in Sardinia Island. Fisheries Research 193, 81–94.
Temporal dynamics of demersal chondrichthyan species in the central western Mediterranean Sea: The case study in Sardinia Island.Crossref | GoogleScholarGoogle Scholar |

Marouani, S., Chaaba, R., Kadri, H., Saidi, B., Bouain, A., Maltagliati, F., Last, P. R., Séret, B., and Bradai, M. N. (2012a). Taxonomic research on Squalus megalops (Macleay, 1881) and Squalus blainvillei (Risso, 1827) (Chondrichthyes: Squalidae) in Tunisian waters (central Mediterranean Sea). Scientia Marina 76, 97–109.
Taxonomic research on Squalus megalops (Macleay, 1881) and Squalus blainvillei (Risso, 1827) (Chondrichthyes: Squalidae) in Tunisian waters (central Mediterranean Sea).Crossref | GoogleScholarGoogle Scholar |

Marouani, S., Kadri, H., Saidi, B., Morize, E., Bouain, A., and Bradai, M. N. (2012b). Age, size, longevity, natural mortality and maturity of the longnose spurdog, Squalus blainvillei (Chondrichthyes: Squalidae), in the Gulf of Gabes (Central Mediterranean Sea). Cahiers de Biologie Marine 53, 197–204.

McPhie, R. P., and Campana, S. E. (2009). Reproductive characteristics and population decline of four species of skate (Rajidae) off the eastern coast of Canada. Journal of Fish Biology 75, 223–246.
Reproductive characteristics and population decline of four species of skate (Rajidae) off the eastern coast of Canada.Crossref | GoogleScholarGoogle Scholar | 20738493PubMed |

Meyers, E. K. M., Tuya, F., Barker, J., Jiménez Alvarado, D., Castro-Hernández, J. J., Haroun, R., and Rödder, D. (2017). Population structure, distribution and habitat use of the critically endangered angelshark, Squatina squatina, in the Canary Islands. Aquatic Conservation: Marine and Freshwater Ecosystem 27, 1133–1144.
Population structure, distribution and habitat use of the critically endangered angelshark, Squatina squatina, in the Canary Islands.Crossref | GoogleScholarGoogle Scholar |

Montealegre-Quijano, S., Cardoso, A. T. C., Silva, R. Z., Kinas, P. G., and Vooren, C. M. (2014). Sexual development, size at maturity, size at maternity and fecundity of the blue shark Prionace glauca (Linnaeus, 1758) in the southwest Atlantic. Fisheries Research 160, 18–32.
Sexual development, size at maturity, size at maternity and fecundity of the blue shark Prionace glauca (Linnaeus, 1758) in the southwest Atlantic.Crossref | GoogleScholarGoogle Scholar |

Moura, T., Serra-Pereira, B., Gordo, L. S., and Figueiredo, I. (2011). Sperm storage in males and females of the deepwater shark Portuguese dogfish with notes on oviducal gland microscopic organization. Journal of Zoology 283, 210–219.
Sperm storage in males and females of the deepwater shark Portuguese dogfish with notes on oviducal gland microscopic organization.Crossref | GoogleScholarGoogle Scholar |

Muñoz-Chápuli, R., and Ramos, H. (1989). Morphological comparison of Squalus blainvillei and S. megalops in the eastern Atlantic, with notes on the genus. Japanese Journal of Ichthyology 36, 6–21.
Morphological comparison of Squalus blainvillei and S. megalops in the eastern Atlantic, with notes on the genus.Crossref | GoogleScholarGoogle Scholar |

Muñoz-Chápuli, R., Ramos, R., and Garcia Garrido, L. (1984). Squalus megalops, McLeay, 1882, en el Mediterraneo. Notas sobre su diagnosis sistematica y distribution. Societat Catalana d'Ictiología i Herpetología 9, 16–21.

Musick, J. A. (1999). Ecology and conservation of long-lived marine animals. In ‘Life in the Slow Lane: Ecology and Conservation of Long-Lived Marine Animals’. (Ed. J. A. Musick.) American Fisheries Society Symposium, Vol. 23, pp. 1–10. (American Fisheries Society: Bethesda, MD, USA.)

Nemeth, R. S. (2005). Population characteristics of a recovering US Virgin Islands red hind spawning aggregation following protection. Marine Ecology Progress Series 286, 81–97.
Population characteristics of a recovering US Virgin Islands red hind spawning aggregation following protection.Crossref | GoogleScholarGoogle Scholar | 16612415PubMed |

Planque, B., Loots, C., Petitgas, P., Lindstrøm, U., and Vaz, S. (2011). Understanding what controls the spatial distribution of fish populations using a multi-model approach. Fisheries Oceanography 20, 1–17.
Understanding what controls the spatial distribution of fish populations using a multi-model approach.Crossref | GoogleScholarGoogle Scholar |

Porcu, C., Marongiu, M. F., Follesa, M. C., Bellodi, A., Mulas, A., Pesci, P., and Cau, A. (2014). Reproductive aspects of the velvet belly Etmopterus spinax (Chondrichthyes: Etmopteridae), from the central western Mediterranean Sea. Notes on gametogenesis and oviducal gland microstructure. Mediterranean Marine Science 15, 313–326.
Reproductive aspects of the velvet belly Etmopterus spinax (Chondrichthyes: Etmopteridae), from the central western Mediterranean Sea. Notes on gametogenesis and oviducal gland microstructure.Crossref | GoogleScholarGoogle Scholar |

Porcu, C., Bellodi, A., Cannas, R., Marongiu, M. F., Mulas, A., and Follesa, M. C. (2015). Life-history traits of the commercial blonde ray, Raja brachyura, from the central-western Mediterranean Sea. Mediterranean Marine Science 16, 90–102.
Life-history traits of the commercial blonde ray, Raja brachyura, from the central-western Mediterranean Sea.Crossref | GoogleScholarGoogle Scholar |

Porcu, C., Bellodi, A., Cau, A., Cannas, R., Marongiu, M. F., Mulas, A., and Follesa, M. C. (2020). Uncommon biological patterns of a little known endemic Mediterranean skate, Raja polystigma (Risso, 1810). Regional Studies in Marine Science 34, 101065.
Uncommon biological patterns of a little known endemic Mediterranean skate, Raja polystigma (Risso, 1810).Crossref | GoogleScholarGoogle Scholar |

Pratt, H. L. (1993). The storage of spermatozoa in the oviducal glands of western North Atlantic sharks. Environmental Biology of Fishes 38, 139–149.
The storage of spermatozoa in the oviducal glands of western North Atlantic sharks.Crossref | GoogleScholarGoogle Scholar |

Pratt, H. L., and Carrier, J. C. (2001). A review of elasmobranch reproductive behavior with a case study on the nurse shark, Ginglymostoma cirratum. Environmental Biology of Fishes 60, 157–188.
A review of elasmobranch reproductive behavior with a case study on the nurse shark, Ginglymostoma cirratum.Crossref | GoogleScholarGoogle Scholar |

Pratt, H. L., and Tanaka, S. (1994). Sperm storage in male elasmobranchs: a description and survey. Journal of Morphology 219, 297–308.
Sperm storage in male elasmobranchs: a description and survey.Crossref | GoogleScholarGoogle Scholar | 8169955PubMed |

Serena, F., Papaconstantinou, C., Relini, G., Gil De Sola, L., and Bertrand, J. A. (2009). Distribution and abundance of spiny dogfish in the Mediterranean Sea based on the Mediterranean International Trawl Survey Program. In ‘Biology and Management of Dogfish Sharks’. (Eds V. F. Gallucci, G. A. McFarlane and G. G. Bargmann.) pp. 139–149. (American Fisheries Society: Bethesda, MD, USA.)

Séret, B., and Serena, F. (2002). The Mediterranean Chondrichthyan fishes (sharks, rays, skates and chimaeras): status and priorities for conservation. United National Environment Programme (UNEP) Regional Activity Centres/Special Protected Areas (RAC/SP) Final Report, Tunis, Tunisia.

Simpfendorfer, C. A., Heupel, M. R., White, W. T., and Dulvy, N. K. (2011). The importance of research and public opinion to conservation management of sharks and rays: a synthesis. Marine and Freshwater Research 62, 518–527.
The importance of research and public opinion to conservation management of sharks and rays: a synthesis.Crossref | GoogleScholarGoogle Scholar |

Sims, D. W. (2005). Differences in habitat selection and reproductive strategies of male and female sharks. In ‘Sexual Segregation in Vertebrates: Ecology of the Two Sexes’. (Eds K. E. Ruckstuhl and P. Neuhaus.) pp. 127–147. (Cambridge University Press: Cambridge, UK.)

Sion, L., D’Onghia, G., Matarrese, A., and Mytilineou, C. (2003). First data on distribution and biology of Squalus blainvillei (Risso, 1826) from the eastern Mediterranean Sea. Journal of Northwest Atlantic Fishery Science 31, 213–219.
First data on distribution and biology of Squalus blainvillei (Risso, 1826) from the eastern Mediterranean Sea.Crossref | GoogleScholarGoogle Scholar |

Smith, R. M., Day, R. W., Walker, T. I., and Hamlett, W. C. (2004). Microscopic organisation of the oviducal gland of the holocephalan elephant fish, Callorhynchus mili. Marine and Freshwater Research 55, 155–164.
Microscopic organisation of the oviducal gland of the holocephalan elephant fish, Callorhynchus mili.Crossref | GoogleScholarGoogle Scholar |

Spare, P., and Venema, S. C. (1992). Introduction to tropical fish stock assessment. Part I. Manual. FAO Fisheries Technical Paper 306, Food and Agriculture Organization of the United Nations, Rome, Italy.

Spedicato, M. T., Massutí, E., Mérigot, B., Tserpes, G., Jadaud, A., and Relini, G. (2019). The MEDITS trawl survey specifications in an ecosystem approach to fishery management. Scientia Marina 83, 9–20.
The MEDITS trawl survey specifications in an ecosystem approach to fishery management.Crossref | GoogleScholarGoogle Scholar |

Stevens, J. D., Bonfil, R., Dulvy, N. K., and Walker, P. A. (2000). The effects of fishing on sharks, rays, and chimaeras (chondrichthyans), and the implications for marine ecosystems. Journal of Marine Science 57, 476–494.
The effects of fishing on sharks, rays, and chimaeras (chondrichthyans), and the implications for marine ecosystems.Crossref | GoogleScholarGoogle Scholar |

Storrie, M. T., Walker, T. I., Laurenson, L. J., and Hamlett, W. C. (2008). Microscopic organization of the sperm storage tubules in the oviducal gland of the female gummy shark (Mustelus antarcticus), with observations on sperm distribution and storage. Journal of Morphology 269, 1308–1324.
Microscopic organization of the sperm storage tubules in the oviducal gland of the female gummy shark (Mustelus antarcticus), with observations on sperm distribution and storage.Crossref | GoogleScholarGoogle Scholar | 18756524PubMed |

Veríssimo, A., Zaera-Perez, D., Leslie, R., Iglésias, S. P., Séret, B., Grigorou, P., Sterioti, A., Gubili, C., Barria, C., Duffy, C., Hernàndez, S., Batjakas, I. E., and Griffiths, A. F. (2017). Molecular diversity and distribution of eastern Atlantic and Mediterranean dogfishes Squalus highlight taxonomic issues in the genus. Zoologica Scripta 46, 414–428.
Molecular diversity and distribution of eastern Atlantic and Mediterranean dogfishes Squalus highlight taxonomic issues in the genus.Crossref | GoogleScholarGoogle Scholar |

Walker, T. I. (2005a). Reproduction in fisheries science. In ‘Reproductive Biology and Phylogeny of Chondrichthyes. Sharks, Batoids and Chimaeras’. (Ed. W. C. Hamlett.) pp. 81–128. (Science Publisher: Plymouth, UK.)

Walker, T. I. (2005b). Management measures. In ‘Management Techniques for Elasmobranchs Fisheries’. (Eds J. A. Musick and R. Bonfil.) FAO Fisheries technical paper number 474, pp. 216–242. (Food and Agriculture Organization of the United Nations: Rome, Italy.)

Walker, T. I., Taylor, B. L., Hudson, R. J., and Cottier, J. P. (1998). The phenomenon of apparent change of growth rate in gummy shark (Mustelus antarcticus). Fisheries Research 39, 139–163.
The phenomenon of apparent change of growth rate in gummy shark (Mustelus antarcticus).Crossref | GoogleScholarGoogle Scholar |

Yano, K., and Tanaka, S. (1988). Size at maturity, reproductive cycle, fecundity and depth segregation of the deep-sea squaloid sharks Centroscymnus owstoni and C. coelolepis in Suruga Bay. Nippon Suisan Gakkaishi 54, 167–174.
Size at maturity, reproductive cycle, fecundity and depth segregation of the deep-sea squaloid sharks Centroscymnus owstoni and C. coelolepis in Suruga Bay.Crossref | GoogleScholarGoogle Scholar |

Zar, J. H. (Ed.) (1999). ‘Biostatistical Analysis’, 4th edn. (Prentice Hall: Upper Saddle River, NJ, USA.)