Phylogenetic diversity within the endemic brown trout Duero lineage: implications for conservation and management
M. Vera A B C , J. L. García-Marín A , P. Martinez B and C. Bouza B
+ Author Affiliations
- Author Affiliations
A Laboratory of Genetic Ichthyology, Department of Biology, Faculty of Sciences, University of Girona, Campus of Montilivi, E-17071 Girona, Spain.
B Department of Genetics, Faculty of Veterinary Science, University of Santiago de Compostela, Campus of Lugo, E-27002 Lugo, Spain.
C Corresponding author. Email: manuel.verar@udg.edu; manuel.vera@usc.es
Marine and Freshwater Research 66(11) 1066-1071 https://doi.org/10.1071/MF14286
Submitted: 17 September 2014 Accepted: 5 December 2014 Published: 22 April 2015
Abstract
Brown trout display great phenotypic and genetic variability. Use of mitochondrial DNA (mtDNA) variation has allowed the definition of seven different lineages in this species to date. One of them, the Duero (DU) lineage, was initially detected in the inner section of the Duero River in Spain, where it showed a parapatric distribution with the more widely distributed Atlantic (AT) lineage. Later mtDNA-RFLP (restriction fragment length polymorphism) studies detected the DU lineage in northern Spanish basins (Galicia). The aim of this work was to ascertain the origin and variability of these DU populations outside the Duero drainage. Using complete mtDNA control region sequencing, 11 novel DU haplotypes were identified. Several of them could be assigned to an endemic group in Galicia consistent with the long-time presence of the DU lineage outside the Duero River, and excluding a recent origin by human translocations. The DU haplotype group observed in north-western Iberian basins was estimated to diverge from that of the Duero River more than 100 000 years ago. We therefore advocate for conservation strategies at regional and local scales rather than focussed in a single ESU as proposed in earlier works.
Additional keywords: Iberian Peninsula, mtDNA, phylogeography.
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