Microbial signatures can help distinguish moon sponges (family Tetillidae) from Darwin Harbour, Australia
Kylie Chambers A , Anna Padovan A C , Belinda Alvarez A B and Karen Gibb A
+ Author Affiliations
- Author Affiliations
A Research Institute for the Environment and Livelihoods, Charles Darwin University, Darwin, NT 0909, Australia.
B Museum and Art Gallery of the Northern Territory, Darwin NT 0801, Australia.
C Corresponding author. Email: anna.padovan@cdu.edu.au
Marine and Freshwater Research 64(8) 716-725 https://doi.org/10.1071/MF12226
Submitted: 21 August 2012 Accepted: 25 March 2013 Published: 21 June 2013
Abstract
The bacterial communities of two sponge morphs collected as part of an ecological study and initially allocated to the genus Paratetilla (Demospongiae: Spirophorida: Tetillidae) were analysed using denaturing gradient gel electrophoresis (DGGE) targeting a region of the 16S rRNA gene. The results showed that the two morphs had different bacterial communities, which suggested that they might be distinct Paratetilla species. The sponge samples were further analysed using conventional taxonomy and cytochrome oxidase I (COI) gene sequencing. These data confirmed that (1) the two morphs belonged to different species, and (2) one morph was more closely related to the tetillid genus Cinachyrella than to Paratetilla.
Additional keywords: COI, DGGE, DNA barcoding, taxonomy.
References
Alvarez, B., and Hooper, J. N. A. (2009). Taxonomic revision of the order Halichondria (Porifera: Demospongiae) from northern Australia. Family Axinellidae. The Beagle, Records of the Museums and Art Galleries of the Northern Territory 25, 17–42.Ferris, M. J., Muyzer, G., and Ward, D. M. (1996). Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community. Applied and Environmental Microbiology 62, 340–346.
| 1:CAS:528:DyaK28XovVCntw%3D%3D&md5=89249e8d10dd5616eead58894a1447b8CAS | 8593039PubMed |
Folmer, O., Black, M., Hoeh, W., Lutz, R., and Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294–299.
| 1:CAS:528:DyaK2MXjt12gtLs%3D&md5=0b1ac07b325bba0e3d8d3405393cf5dfCAS | 7881515PubMed |
Hentschel, U., Hopke, J., Horn, M., Fredrich, A. B., Wagner, M., Hacker, J., and Moore, B. (2002). Molecular evidence of a uniform microbial community in sponges from different oceans. Applied and Environmental Microbiology 68, 4431–4440.
| Molecular evidence of a uniform microbial community in sponges from different oceans.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XmvVCjsrs%3D&md5=3325204588f40020502d8ec9ca757f3bCAS | 12200297PubMed |
Hooper, J. N. A., Kennedy, J. A., and Quinn, R. (2002). Biodiversity ‘hotspots’, patterns of richness, and taxonomic affinities of tropical Australian sponges (Porifera). Biodiversity and Conservation 11, 851–885.
| Biodiversity ‘hotspots’, patterns of richness, and taxonomic affinities of tropical Australian sponges (Porifera).Crossref | GoogleScholarGoogle Scholar |
Hornlein, C., Becking, L., Erpenbeck, D., De Voogd, N. J., and Santodomingo, N. (2010). The complexity of the moons: on the phylogeny and phylogeography of Tetillidae in Indonesian waters. In ‘VIII World Sponge Conference 2010, pg. 219, Girona, Spain, 20–24 September’.
Meyer, B., and Kuever, J. (2008). Phylogenetic diversity and spatial distribution of the microbial community associated with the Caribbean deep-water sponge Polymastia cf. corticata by rRNA aprA, and amoA gene analysis. Microbial Ecology 56, 306–321.
| Phylogenetic diversity and spatial distribution of the microbial community associated with the Caribbean deep-water sponge Polymastia cf. corticata by rRNA aprA, and amoA gene analysis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXnvVOnsLw%3D&md5=316f1859a6f1aef61f2ad2972c9397abCAS | 18193317PubMed |
Muyzer, G., de Waal, E. C., and Uitterlinden, A. G. (1993). Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Applied and Environmental Microbiology 59, 695–700.
| 1:CAS:528:DyaK3sXit1Kktrk%3D&md5=9d0361118b0a2faec65ba3935bc31741CAS | 7683183PubMed |
Padovan, A. C., Munksgaard, N. C., Alvarez, B., McGuinness, K., Parry, D. L., and Gibb, K. S. (2012). Bioconcentration of metals by the tropical sponge, Spheciospongia vagabunda: synchrotron X-ray imaging reveals the micron-scale distribution of accumulated metals. Hydrobiologia , .
| Bioconcentration of metals by the tropical sponge, Spheciospongia vagabunda: synchrotron X-ray imaging reveals the micron-scale distribution of accumulated metals.Crossref | GoogleScholarGoogle Scholar |
Page, R. D. M. (1996). TreeView: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12, 357–358. Available at http://taxonomy.zoology.gla.ac.uk/rod/rod.html [accessed 29 October 2011]
Radwan, M., Hanora, A., Zan, J., Mohamed, N. M., Abo-Elmatty, D. M., Abou-El-Ela, S. H., and Hill, R. T. (2010). Bacterial community analysis of two red sea sponges. Marine Biotechnology 12, 350–360.
| Bacterial community analysis of two red sea sponges.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXmsFSqtL4%3D&md5=a35f009b33abe32061dc850225497fdaCAS | 19957096PubMed |
Rot, C., Goldfarb, I., Ilan, M., and Huchon, D. (2006). Putative cross-kingdom horizontal gene transfer in sponge (Porifera) mitochondria. BMC Evolutionary Biology 6, .
| Putative cross-kingdom horizontal gene transfer in sponge (Porifera) mitochondria.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtFGisbfN&md5=418cd9f84f88e32849d435c6e01d2e76CAS | 16972986PubMed |
Rützler, K. (1987). Tetillidae (Spirophorida, Porifera): a taxonomic reevaluation. Taxonomy of Porifera. , 187–203 (Springer-Verlag, Berlin, Heidelberg).
Santodomingo, N. K., Becking, L. E., de Voogd, N. J., and van Soest, R. W. M. (2010). Unravelling the moons: taxonomic insights of Tetillidae in Indonesian waters. In ‘VIII World Sponge Conference 2010, pg. 323, Girona, Spain, 20–24 September 2010’.
Schmitt, S., Tsai, P., Bell, J., Fromont, J., Ilan, M., Lindquist, N., Perez, T., Rodrigo, A., Schupp, P., Vacelet, J., Webster, N., Hentschel, U., and Taylor, M. W. (2011). Assessing the complex sponge microbiota: core, variable and species-specific bacterial communities in marine sponges. The ISME Journal 6, 564–576.
| Assessing the complex sponge microbiota: core, variable and species-specific bacterial communities in marine sponges.Crossref | GoogleScholarGoogle Scholar | 21993395PubMed |
Szitenberg, A., Rot, C., Ilan, M., and Huchon, D. (2010). Diversity of sponge mitochondrial introns revealed by cox 1 sequences of Tetillidae. BMC Evolutionary Biology 10, .
| Diversity of sponge mitochondrial introns revealed by cox 1 sequences of Tetillidae.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtF2rs7nP&md5=fb4e70578658be713ecd31c733342ae1CAS | 20849667PubMed |
Taylor, M. W., Radax, R., Steger, D., and Wagner, M. (2007). Sponge associated microorganisms: Evolution, ecology and biotechnology potential. Microbiology and Molecular Biology Reviews 71, 295–347.
| Sponge associated microorganisms: Evolution, ecology and biotechnology potential.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXotV2gu7w%3D&md5=58c3df8eaa64f5aefb4894761229a7b1CAS | 17554047PubMed |
Van Soest, R., and Rützler, K. (2002). Family Tetillidae Sollas, 1886. In ‘SystemaPorifera: a Guide to the Classification of Sponges’. (Eds J. N. A. Hooper and R. W. M. Van Soest.) pp. 85–98. (Kluwer Academic/Plenum Publishers: New York.)
Webster, N. S., Luter, H., Battershill, C., and Whalan, S. (2010). Great Barrier Reef sponges: symbionts variability between and within different host species. In ‘VIII World Sponge Conference 2010, pg. 364, Girona, Spain, 20–24 September 2010’.
Weisz, J. B., Hentschel, U., Lindquist, N., and Martens, C. S. (2007). Linking abundance and diversity of sponge-associated microbial communities to metabolic differences in host sponges. Marine Biology 152, 475–483.
| Linking abundance and diversity of sponge-associated microbial communities to metabolic differences in host sponges.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXotVOgtbs%3D&md5=5510413203b87cfac56336ebdc824fcdCAS |
