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RESEARCH ARTICLE

Rapid responses of pristine marine planktonic communities in experimental approach to diuron and naphthalene (Juan de Nova Island, Western Indian Ocean)

Marc Bouvy https://orcid.org/0000-0002-1092-3269 A F , Christine Dupuy B , Patrice Got A , Isabelle Domaizon C , Claire Carré A , Marc Pagano D , Didier Debroas E , Cécile Roques A and Christophe Leboulanger A
+ Author Affiliations
- Author Affiliations

A UMR 9190 MARBEC, Université de Montpellier-IRD-Ifremer-CNRS, Place Eugène Bataillon, Case 093, F-34095 Montpellier cedex 5, France.

B UMR 7266 Littoral, Environnement et Sociétés (LIENSs), CNRS-Université de La Rochelle, 2 Rue Olympe de Gouges, F-17000 La Rochelle cedex, France.

C UMR 042 CARRTEL, INRAE-Université Savoie M2ont Blanc, 75 Avenue de Corzent, F-74203 Thonon-les-Bains cedex, France.

D UMR 235 Mediterranean Institute of Oceanography (MIO), IRD-CNRS-Université de Toulon-Université Aix-Marseille, F-13288 Marseille cedex 09, France.

E UMR 6023 Microorganismes Génome et Environnement, CNRS-Université Clermont Auvergne, 24 Avenue des Landais, F-63177 Aubière cedex, France.

F Corresponding author. Email: marc.bouvy@ird.fr

Marine and Freshwater Research 72(7) 1065-1085 https://doi.org/10.1071/MF20276
Submitted: 14 September 2020  Accepted: 9 January 2021   Published: 5 February 2021

Abstract

Planktonic communities from Juan de Nova lagoon were submitted to a single exposure of diuron or naphthalene in triplicate outdoor 40-L mesocosms for 5 days. The study followed the temporal changes of the assemblages by determining abundances and taxonomic level of microbial (heterotrophic prokaryotes, picocyanobacteria, heterotrophic protists and phytoplankton) and metazooplankton communities. Exposure to both contaminants did not result in significant changes in phytoplankton abundances. Microscopic identification demonstrated the dominance of Bacillariophyta (mostly Chaetoceros tenuissimus) accounting for more than 95% of the microalgae detected in all mesocosms. Using 18S rRNA metabarcoding, temporal changes in the proportion of eukaryotic operational taxonomic units (OTUs) were highlighted for most taxonomic groups. Diuron had no significant effect on the abundance of eukaryotic picoheterotrophs, ciliates, whereas naphthalene had a significant positive effect on heterotrophic prokaryotes. Metazooplankton was not significantly modified under contaminant exposure, except for copepods nauplii in presence of diuron. Multivariate analysis indicated significant responses of communities linked to the experimental time and conditions. The analysis also revealed a modification of the trophic relationships among communities over time, from a dominant multivorous food web after 48 h towards a reduced microbial loop after 120 h of experiment time.

Keywords: contaminant, taxonomy, metabarcoding, plankton, experiments, lagoon.


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