Hybridisation experiments between freshwater prawns Macrobrachium amazonicum and M. pantanalense (Decapoda: Palaemonidae), and the effects of geographical isolation
Caio Santos Nogueira
A C , João Alberto Farinelli Pantaleão B and Rogerio Caetano Costa A
+ Author Affiliations
- Author Affiliations
A Laboratory of Biology of Marine and Freshwater Shrimps (LABCAM), Department of Biological Sciences, Faculty of Sciences, São Paulo State University (UNESP), Avenue Engenheiro Luiz Edmundo Carrijo Coube 14-01, 17033-360, São Paulo, Brazil.
B Laboratory of Bioecology and Systematics of Crustacean (LBSC), Department of Biology, Faculty of Philosophy, Science and Letters at Ribeirão Preto (FFCLRP), University of São Paulo (USP), Avenue Bandeirantes 3900, 14040-901, Ribeirão Preto, São Paulo, Brazil.
C Corresponding author. Email: caiosnogueira@hotmail.com
Marine and Freshwater Research 72(4) 520-525 https://doi.org/10.1071/MF20226
Submitted: 18 July 2020 Accepted: 25 August 2020 Published: 29 September 2020
Abstract
The freshwater prawns Macrobrachium amazonicum and M. pantanalense are phylogenetically closely related. The present study analysed the occurrence of copulation between these two species. Specimens of M. amazonicum were collected from the Tietê River in Cambaratiba (SP), whereas M. pantanalense specimens were collected from the Lagoa Baíazinha, in Miranda (MS). Six different experimental groups were used, MA♂ × MP♀, MA♀ × MP♂ (interspecifc), MA♂ × MA♀, MP♂ × MP♀ (intraspecific), MA♀ and MP♀ (individualised; MA = M. amazonicum and MP = M. pantanalense). The incubation times of the ovigerous females were monitored in all experiments. Some females from all experimental groups were randomly selected and had their oocytes photographed to verify the occurrence of embryonic development. No larvae hatched in the interspecific experimental groups, whereas, in intraspecific groups, almost all experiments (95%) presented larval hatching. The incubation period differed significantly (P < 0.001) between the intraspecific groups and all the other experimental groups, but not between each other (P > 0.05). The interspecific and individualised groups showed no significant (P > 0.05) difference between them. In addition, oocytes from the interspecific experimental groups did not present characteristics of embryonic development. Our results suggest that M. pantanalense and M. amazonicum populations do not copulate, which corroborates the proposal of recent speciation.
Keywords: genetic distance, intraspecific recognition, pantanal, reproductive barrier, sexual behavior.
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