Unresolved diversity and monthly dynamics of eukaryotic phytoplankton in a temperate freshwater reservoir explored by pyrosequencing
Thangavelu Boopathi A and Jang-Seu Ki A B C
+ Author Affiliations
- Author Affiliations
A Institute of Natural Sciences, Sangmyung University, Seoul 03016, South Korea.
B Department of Life Science, Sangmyung University, Seoul 03016, South Korea.
C Corresponding author. Email: kijs@smu.ac.kr
Marine and Freshwater Research 67(11) 1680-1691 https://doi.org/10.1071/MF15136
Submitted: 6 April 2015 Accepted: 3 August 2015 Published: 27 October 2015
Abstract
Environmental monitoring of the succession of phytoplankton communities in freshwater ecosystems is critical in efficient water quality management. In the present study we analysed the monthly dynamics of eukaryotic phytoplankton diversity and community structure in a freshwater reservoir (Paldang Reservoir, South Korea) using small subunit (SSU) rRNA pyrosequencing. We analysed physicochemical and biological parameters of water samples collected at monthly intervals from March 2012 to February 2013. The occurrence of phytoplankton exhibited a monthly variation: low in September (13.2%) and February (9.7%) and high in May (75.5%), July (76.5%), August (86.3%) and November (70.6%). We observed greater phytoplankton diversity predominantly represented by diatoms (37.4%), dinoflagellates (29%), cryptophytes (16%) and chlorophytes (10.4%). In early and mid-spring, diatoms, particularly Stephanodiscus spp., were dominant; however, in late spring and early summer the cryptophyte Cryptomonas spp. was dominant; the dinoflagellate Peridionopsis sp. was dominant in late summer and late autumn. Overall, the molecular results of the present study represent a typical pattern of seasonal phytoplankton succession in temperate regions. Pyrosequencing detected more phytoplankton taxa that were unresolved under microscopy, suggesting the usefulness of this method in continuous monitoring of phytoplankton communities in freshwater ecosystems.
Additional keywords: Paldang Reservoir, small subunit rRNA, SSU.
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