The dynamics of attached and free-living bacterial population in tropical coastal waters
Siew Wen Lee A , Choon Weng Lee A B F , Chui Wei Bong A B , Kumaran Narayanan C D and Edmund Ui-Hang Sim E
+ Author Affiliations
- Author Affiliations
A Laboratory of Microbial Ecology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
B Institute of Ocean and Earth Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia.
C Monash University Malaysia, Bandar Sunway, 46150 Selangor Darul Ehsan, Malaysia.
D Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USA.
E Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Malaysia.
F Corresponding author. Email: lee@um.edu.my
Marine and Freshwater Research 66(8) 701-710 https://doi.org/10.1071/MF14123
Submitted: 13 March 2014 Accepted: 19 August 2014 Published: 25 February 2015
Abstract
We investigated the dynamics of attached and free-living bacterial abundance over a period of 18 months in tropical coastal waters of Malaysia. We measured the abundance at both oligotrophic coastal water (Port Dickson) and eutrophic estuary (Klang), and hypothesised that attached bacteria are predominant in eutrophic waters. We found that bacterial abundance was higher at Klang than Port Dickson (Student’s t-test: t = 4.87, d.f. = 19, P < 0.001). Attached bacteria also formed a large fraction of the total bacteria at Klang (75% ± 13 s.d.) relative to Port Dickson (56% ± 22), and showed preference for chlorophyll-a-based particles rather than total suspended solids. The bacterial community structure was clearly different between the two stations but was similar between the attached and free-living bacterial population. Our results showed the importance of attached bacteria in eutrophic water where they could play a major role in carbon and nutrient cycling.
Additional keywords: 16S rRNA PCR–DGGE, LIVE/DEAD bacterial staining, Straits of Malacca.
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