Next generation sequencing in single cell parasite disease investigations
Jan ŠlapetaParasitology Laboratory
McMaster Building B14
Faculty of Veterinary Science
University of Sydney
NSW 2006, Australia
Email: jan.slapeta@sydney.edu.au
Microbiology Australia 34(4) 192-193 https://doi.org/10.1071/MA13067
Published: 18 September 2013
Abstract
Single cell parasites, also referred as protozoa, are ubiquitous. They are parasites of animals and humans, causing significant disease such as malaria and toxoplasmosis. In farm animal medicine and human medicine, specific diagnostic tests have been developed to detect many of these diseases. Unfortunately, the role of protozoal agents in wildlife disease is poorly understood and diagnosis is confounded by the lack of basic knowledge of parasite distribution and morphological identification. Therefore we are pursuing a new approach using 'state of the art' next generation sequencing to address existing limitations. The approach relies on microbial community DNA analysis as a faster and more economical method compared with development of traditional species-specific diagnostic methodologies.
References
[1] Adlard, R.D. and O’Donoghue, P.J. (1998) Perspectives on the biodiversity of parasitic protozoa in Australia. Int. J. Parasitol. 28, 887–897.| Perspectives on the biodiversity of parasitic protozoa in Australia.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK1czjvVKmtA%3D%3D&md5=eae583d407794086a5e996f11b8c51d1CAS | 9673868PubMed |
[2] Zhu, B.Y. et al. (2009) Looks can deceive: molecular identity of an intraerythrocytic apicomplexan parasite in Australian gliders. Vet. Parasitol. 159, 105–111.
| Looks can deceive: molecular identity of an intraerythrocytic apicomplexan parasite in Australian gliders.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXnsVSquw%3D%3D&md5=c3a998bf1453dc1cd1c9ef1152d8c832CAS | 19028015PubMed |
[3] Hartigan, A. et al. (2012) Myxozoan parasite in brain of critically endangered frog. Emerg. Infect. Dis. 18, 693–695.
| Myxozoan parasite in brain of critically endangered frog.Crossref | GoogleScholarGoogle Scholar | 22469079PubMed |
[4] Miller, M.A. et al. (2008) Type X Toxoplasma gondii in a wild mussel and terrestrial carnivores from coastal California: new linkages between terrestrial mammals, runoff and toxoplasmosis of sea otters. Int. J. Parasitol. 38, 1319–1328.
| Type X Toxoplasma gondii in a wild mussel and terrestrial carnivores from coastal California: new linkages between terrestrial mammals, runoff and toxoplasmosis of sea otters.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXps12isrw%3D&md5=ff190da679ef736fc864d62cc2e6195cCAS | 18452923PubMed |
[5] Sun, Y. et al. (2011) Tag-encoded FLX amplicon pyrosequencing for the elucidation of microbial and functional gene diversity in any environment. Methods Mol. Biol. 733, 129–141.
| Tag-encoded FLX amplicon pyrosequencing for the elucidation of microbial and functional gene diversity in any environment.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXntVChur8%3D&md5=7edacd60f642a568a36e8b34eafade08CAS | 21431767PubMed |
[6] Šlapeta, J. and Linares, M. (2013) Combined amplicon pyrosequencing assays reveal presence of the apicomplexan “type-N” (cf. Gemmocystis cylindrus) and Chromera velia on the Great Barrier Reef, Australia. PLoS ONE , .
| Combined amplicon pyrosequencing assays reveal presence of the apicomplexan “type-N” (cf. Gemmocystis cylindrus) and Chromera velia on the Great Barrier Reef, Australia.Crossref | GoogleScholarGoogle Scholar |
[7] Vogelnest, L. and Woods, R., eds (2008) Medicine of Australian Mammals, CSIRO Publishing.
[8] Debenham, J.J. et al. (2012) Year-long presence of Eimeria echidnae and absence of Eimeria tachyglossi in captive short-beaked echidnas (Tachyglossus aculeatus). J. Parasitol. 98, 543–549.
| Year-long presence of Eimeria echidnae and absence of Eimeria tachyglossi in captive short-beaked echidnas (Tachyglossus aculeatus).Crossref | GoogleScholarGoogle Scholar | 22236183PubMed |
[9] Margulies, M. et al. (2005) Genome sequencing in microfabricated high-density picolitre reactors. Nature 437, 376–380.
| 1:CAS:528:DC%2BD2MXpvFOrt7s%3D&md5=dea90f3c1f833bc33f050a7be0550989CAS | 16056220PubMed |
[10] Amaral-Zettler, L.A. et al. (2011) Microbial community structure across the tree of life in the extreme Rio Tinto. ISME J. 5, 42–50.
| Microbial community structure across the tree of life in the extreme Rio Tinto.Crossref | GoogleScholarGoogle Scholar | 20631808PubMed |
[11] Cheung, M.K. et al. (2010) Composition and genetic diversity of picoeukaryotes in subtropical coastal waters as revealed by 454 pyrosequencing. ISME J. 4, 1053–1059.
| Composition and genetic diversity of picoeukaryotes in subtropical coastal waters as revealed by 454 pyrosequencing.Crossref | GoogleScholarGoogle Scholar | 20336159PubMed |
[12] Stoeck, T. et al. (2009) Massively parallel tag sequencing reveals the complexity of anaerobic marine protistan communities. BMC Biol. 7, 72.
| Massively parallel tag sequencing reveals the complexity of anaerobic marine protistan communities.Crossref | GoogleScholarGoogle Scholar | 19886985PubMed |
