Identification of bacteria from aquatic animals
Nicky Buller A B and Sam Hair A
Department of Agriculture and Food Western Australia
3 Baron-Hay Court
South Perth, WA 6151, Australia
Microbiology Australia 37(3) 129-131 https://doi.org/10.1071/MA16044
Published: 22 August 2016
Abstract
A wide range of aquatic animal species are cultured for human consumption, the fashion industry, research purposes or re-stocking natural populations. Each host species may be colonised by bacterial saprophytes or infected with pathogens that have specific growth requirements encompassing temperature, salinity, trace elements or ions. To ensure successful culture and identification of potential pathogens, the microbiologist must have in-depth knowledge of these growth requirements and access to the appropriate resources. Identification techniques include traditional culture and biochemical identification methods modified to take into account any growth requirements, identification using mass spectrometry, detection of nucleic acids, sequencing 16S rRNA or specific genes, and whole genome sequencing.
References
[1] Buller, N.B. (2014) Bacteria and Fungi from Fish and other Aquatic Animals; a practical identification manual. CABI, Oxfordshire, UK.[2] Wakabayashi, H. et al. (1986) Flexibacter maritimus sp. nov., a pathogen of marine fishes. Int. J. Syst. Bacteriol. 36, 396–398.
| Flexibacter maritimus sp. nov., a pathogen of marine fishes.Crossref | GoogleScholarGoogle Scholar |
[3] Anacker, R.L. and Ordal, E.J. (1959) Studies on the myxobacterium Chondrococcus columnaris. I. Serological typing. J. Bacteriol. 78, 25–32.
| 1:STN:280:DyaG1M7gt1KqtQ%3D%3D&md5=5f16efc9ed16389fcbcc09888e4e372bCAS | 13672906PubMed |
[4] Cipriano, R.C. and Holt, R.A. (2005) Flavobacterium psychrophilum, cause of bacterial cold-water disease and rainbow trout fry syndrome. Fish Disease leaflet No. 86. National Fish Health Research Laboratory.
[5] Fryer, J.L. and Sanders, J.E. (1981) Bacterial Kidney Disease of salmonid fish. Annu. Rev. Microbiol. 35, 273–298.
| Bacterial Kidney Disease of salmonid fish.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaL38%2FksVGlsA%3D%3D&md5=de940e6ad2508d1f0e6b0f4a7346947aCAS | 6794423PubMed |
[6] Cowan, S.T. and Steel, K.J. (1970) Manual for the identification of medical bacteria. Cambridge: Cambridge University Press.
[7] West, P.A. and Colwell, R.R. (1984) Identification and classification of Vibrionaceae – an overview. In Vibrios in the Environment. New York: John Wiley & Sons, pp. 285–363.
[8] Clarridge, J.E. and Zighelboim-Daum, S. (1985) Isolation and characterization of two hemolytic phenotypes of Vibrio damsela associated with a fatal wound infection. J. Clin. Microbiol. 21, 302–306.
| 1:STN:280:DyaL2M7lvVWhtw%3D%3D&md5=cf84e286b8809c99f4f19e9da0c939deCAS | 3980686PubMed |
[9] Croci, L. et al. (2007) Comparison of different biochemical and molecular methods for the identification of Vibrio parahaemolyticus. J. Appl. Microbiol. 102, 229–237.
| Comparison of different biochemical and molecular methods for the identification of Vibrio parahaemolyticus.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXitlOmt7c%3D&md5=1026fb6e73b7828a94377132c4bd0f87CAS | 17184339PubMed |
[10] Haenen, O.L.M. et al. (2013) Bacterial infections from aquatic species: potential for and prevention of contact zoonoses. Rev. Sci. Tech. 32, 497–507.
| Bacterial infections from aquatic species: potential for and prevention of contact zoonoses.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC2cvmtFOjtg%3D%3D&md5=823ffb474e5a6ce52a5ff9945943cb33CAS |
[11] Griffin, P.M. et al. (2012) Use of matrix-assisted laser desorption ionization – time of flight mass spectrometry to identify vancomycin-resistance Enterococci and investigate the epidemiology of an outbreak. J. Clin. Microbiol. 50, 2918–2931.
| Use of matrix-assisted laser desorption ionization – time of flight mass spectrometry to identify vancomycin-resistance Enterococci and investigate the epidemiology of an outbreak.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhsVarsb3E&md5=4ec6026fdffd2c45a983f749abe21747CAS | 22740710PubMed |
[12] Randall, L.P. et al. (2015) Evaluation of MALDI-TOF as a method for the identification of bacteria in the veterinary diagnostic laboratory. Res. Vet. Sci. 101, 42–49.
| Evaluation of MALDI-TOF as a method for the identification of bacteria in the veterinary diagnostic laboratory.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXhtVegur3P&md5=5da23954cc1c0c4866ff2e9835afd857CAS | 26267088PubMed |
[13] Dieckmann, R. et al. (2010) Rapid identification and characterization of Vibrio species using whole-cell MALDI-TOF mass spectrometry. J. Appl. Microbiol. 109, 199–211.
| 1:CAS:528:DC%2BC3cXpslOrsr0%3D&md5=55153df484a4a6fe3eab7d84c2ac58a1CAS | 20059616PubMed |
[14] Sawabe, T. et al. (2007) Inferring the evolutionary history of vibrios by means of multilocus sequence analysis. J. Bacteriol. 189, 7932–7936.
| Inferring the evolutionary history of vibrios by means of multilocus sequence analysis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXht1Knu7zK&md5=ee6c5e2d8d6fce9e5d97c7c64188f665CAS | 17704223PubMed |
[15] Martínez-Murcia, A.J. et al. (2011) Multilocus phylogenetic analysis of the genus Aeromonas. Syst. Appl. Microbiol. 34, 189–199.
| Multilocus phylogenetic analysis of the genus Aeromonas.Crossref | GoogleScholarGoogle Scholar | 21353754PubMed |