Wastewater, wheat and table wipes: adventures in culture-independent microbiology
Jacob E Munro A , Deborah J Rich A , Simon Dingsdag A and Nicholas V Coleman A B
+ Author Affiliations
- Author Affiliations
A School of Molecular Bioscience
Building G08, University of Sydney
Darlington, NSW 2006, Australia.
B Corresponding author. Tel: +61 2 9351 6047
Fax: +61 2 9351 5858
Email: nicholas.coleman@sydney.edu.au
Microbiology Australia 35(4) 188-191 https://doi.org/10.1071/MA14061
Published: 30 October 2014
Abstract
The sequencing of ribosomal RNA and DNA (rRNA/rDNA) from environmental samples heralded a new age in microbiology1–3. The advent of next-generation sequencing supercharged these methods, which now give high-resolution data sets, enabling real insights into microbial diversity and function in complex systems4–7. Here, three local applications of 16S rDNA pyrosequencing are described, which highlight the usefulness of this approach for addressing practical questions in diverse areas of microbiology. Limitations of the sequence-based approach will also be discussed.
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