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RESEARCH ARTICLE

Taxonomic reassessment of Scabies (Bivalvia: Unionidae) species in China based on multilocus and mitogenomic phylogenetic analyses

Yu-Ting Dai https://orcid.org/0009-0001-5856-3987 A # , Zhong-Guang Chen A # , Cheng-Lin Hu A , Peng-Fei Ning B , Shan Ouyang A , Xiao-Chen Huang https://orcid.org/0000-0002-0242-3571 A * and Xiao-Ping Wu https://orcid.org/0000-0002-8037-5640 A *
+ Author Affiliations
- Author Affiliations

A School of Life Sciences, Nanchang University, Nanchang, 330031, PR China.

B Fisheries Research Institute of Tianjin, Tianjin, 300221, PR China.

# These authors contributed equally to this paper

Handling Editor: Gonzalo Giribet

Invertebrate Systematics 38, IS24020 https://doi.org/10.1071/IS24020
Submitted: 27 February 2024  Accepted: 8 May 2024  Published: 17 June 2024

© 2024 The Author(s) (or their employer(s)). Published by CSIRO Publishing.

Abstract

Effective species conservation necessitates the ability to accurately differentiate among species, a challenge compounded by taxonomic uncertainties in freshwater mussels due to substantial intraspecific variation and pronounced phenotypic plasticity in shell morphology. The taxonomic status and species validity of Scabies longata and S. chinensis, two species endemic in China, have been under continuous debate since establishment. The lack of essential molecular data required for a comprehensive systematic study has resulted in the unresolved taxonomic status of these two species. This study presents molecular data, including COI barcoding, COI + 28S rRNA, and mitogenomic data combined with morphological characteristics to assess the validity of S. longata and S. chinensis. Both morphological and COI barcoding data support the conclusion that S. longata and S. chinensis are junior synonyms of Nodularia douglasiae and N. nuxpersicae respectively. Our findings suggest the absence of Scabies species in China. Mitochondrial phylogenetic analyses were used to further elucidate intrageneric relationships within the genus Nodularia, revealing the following relationships: (N. breviconcha (Nodularia sp. 1 (N. douglasiae (N. nuxpersicae, N. nipponensis)))). We underscore the significance of employing an integrated taxonomic approach for species identification, especially given the considerable morphological disparities between larvae and adult freshwater mussels. Proper morphological identification of adult specimens is essential for extracting meaningful taxonomic characters. Furthermore, our findings suggest a notable resemblance between the freshwater bivalve fauna in southern China and those east of the Mekong River.

ZooBank: urn:lsid:zoobank.org:pub:DA87D330-5E23-4F4B-8CC2-CBA3CD191BE8

Keywords: comparator species, conservation, freshwater mussels, integrative taxonomy, mitochondrial phylogenomics, morphological characters, multilocus phylogeny, Nodularia.

References

Allendorf FW, Hohenlohe PA, Luikart G (2010) Genomics and the future of conservation genetics. Nature Reviews Genetics 11(10), 697-709.
| Crossref | Google Scholar | PubMed |

Avise JC (2000) ‘Phylogeography: the history and formation of species.’ (Harvard University Press: Cambridge, MA, USA)

Bernt M, Donath A, Jühling F, Externbrink F, Florentz C, Fritzsch G, Pütz J, Middendorf M, Stadler PF (2013) MITOS: improved de novo metazoan mitochondrial genome annotation. Molecular Phylogenetics and Evolution 69(2), 313-319.
| Crossref | Google Scholar | PubMed |

Bieler R, Mikkelsen PM, Collins TM, Glover EA, González VL, Graf DL, Harper EM, Healy J, Kawauchi GY, Sharma PP, Staubach S, Strong EE, Taylor JD, Tëmkin I, Zardus JD, Clark S, Guzmán A, McIntyre E, Sharp P, Giribet G (2014) Investigating the Bivalve Tree of Life – an exemplar-based approach combining molecular and novel morphological characters. Invertebrate Systematics 28(1), 32-115.
| Crossref | Google Scholar |

Bogan AE, Allen DJ (2021) Nodularia douglasiae. The ‘IUCN Red List of Threatened Species 2021’. e.T166285A98189479. (International Union for Conservation of Nature and Natural Resources) Available at https://www.iucnredlist.org/species/166285/98189479

Böhm M, Dewhurst-Richman NI, Seddon M, Ledger SEH, Albrecht C, Allen D, Bogan AE, Cordeiro J, Cummings KS, Cuttelod A, Darrigran G, Darwall W, Fehér Z, Gibson C, Graf DL, Köhler F, Lopes-Lima M, Pastorino G, Perez KE, Smith K, van Damme D, Vinarski MV, von Proschwitz T, von Rintelen T, Aldridge DC, Aravind NA, Budha PB, Clavijo C, Van Tu D, Gargominy O, Ghamizi M, Haase M, Hilton-Taylor C, Johnson PD, Kebapçı Ü, Lajtner J, Lange CN, Lepitzki DAW, Martínez-Ortí A, Moorkens EA, Neubert E, Pollock CM, Prié V, Radea C, Ramirez R, Ramos MA, Santos SB, Slapnik R, Son MO, Stensgaard AS, Collen B (2021) The conservation status of the world’s freshwater molluscs. Hydrobiologia 848(12), 3231-3254.
| Crossref | Google Scholar |

Bolotov IN, Pfeiffer JM, Konopleva ES, Vikhrev IV, Kondakov AV, Aksenova OV, Gofarov MY, Tumpeesuwan S, Win T (2018) A new genus and tribe of freshwater mussel (Unionidae) from Southeast Asia. Scientific Reports 8(1), 10030.
| Crossref | Google Scholar |

Bolotov IN, Konopleva ES, Vikhrev IV, Gofarov MY, Lopes-Lima M, Bogan AE, Lunn Z, Chan N, Win T, Aksenova OV, Tomilova AA, Tanmuangpak K, Tumpeesuwan S, Kondakov AV (2020) New freshwater mussel taxa discoveries clarify biogeographic division of Southeast Asia. Scientific Reports 10(1), 6616.
| Crossref | Google Scholar | PubMed |

Brandt RAM (1974) The non-marine aquatic Mollusca of Thailand. Archiv für Molluskenkunde 105, 1-423.
| Google Scholar |

Breton S, Beaupré HD, Stewart DT, Hoeh WR, Blier PU (2007) The unusual system of doubly uniparental inheritance of mtDNA: isn’t one enough? Trends in Genetics 23, 465-474.
| Crossref | Google Scholar | PubMed |

Castresana J (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution 17(4), 540-552.
| Crossref | Google Scholar | PubMed |

Choi EH, Kim G, Cha SH, Lee JS, Ryu SH, Suk HY, Lee YS, Baek SY, Hwang UW (2020) Molecular phylogenetic, population genetic and demographic studies of Nodularia douglasiae and Nodularia breviconcha based on CO1 and 16S rRNA. Scientific Reports 10(1), 16572.
| Crossref | Google Scholar |

Clement M, Posada D, Crandall KA (2000) TCS: a computer program to estimate gene genealogies. Molecular Ecology 9(10), 1657-1659.
| Crossref | Google Scholar | PubMed |

Dai YT, Huang XC, Wu CHZ, Chen ZG, Guo L, Shu FY, Ouyang S, Wu XP (2023) Multilocus and mitogenomic phylogenetic analyses reveal a new genus and species of freshwater mussel (Bivalvia: Unionidae) from Guangxi, China. Invertebrate Systematics 37(2), 152-166.
| Crossref | Google Scholar |

Dai YT, Chen ZG, Peng KJ, Ouyang S, Huang XC, Wu XP (2024) Revisiting the genus Pseudocuneopsis (Bivalvia, Unionidae): morphology, mitochondrial phylogenomics, and the description of a new species. Zoologica Scripta 53, 323-337.
| Crossref | Google Scholar |

Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32(5), 1792-1797.
| Crossref | Google Scholar | PubMed |

Edler D, Klein J, Antonelli A, Silvestro D (2020) raxmlGUI 2.0: a graphical interface and toolkit for phylogenetic analyses using RAxML. Methods in Ecology and Evolution 12(2), 373-377.
| Crossref | Google Scholar |

Froufe E, Bolotov I, Aldridge DC, Bogan AE, Breton S, Gan HM, Kovitvadhi U, Kovitvadhi S, Riccardi N, Secci-Petretto G, Sousa R, Teixeira A, Varandas S, Zanatta D, Zieritz A, Fonseca MM, Lopes-Lima M (2020) Mesozoic mitogenome rearrangements and freshwater mussel (Bivalvia: Unionoidea) macroevolution. Heredity 124(1), 182-196.
| Crossref | Google Scholar | PubMed |

Gates KK, Vaughn CC, Julian JP (2015) Developing environmental flow recommendations for freshwater mussels using the biological traits of species guilds. Freshwater Biology 60(4), 620-635.
| Crossref | Google Scholar |

Goncalves A, Zieritz A, Lopes-Lima M, Deein G, Pfeiffer J (2022) Taxonomic revision and conservation assessment of the Southeast Asian freshwater mussel genus Chamberlainia Simpson, 1900. Journal of Molluscan Studies 88(2), eyac008.
| Crossref | Google Scholar |

Graf DL (2007) Palearctic freshwater mussel (Mollusca: Bivalvia: Unionoida) diversity and the comparatory method as a species concept. Proceedings of the Academy of Natural Sciences of Philadelphia 156(1), 71-88.
| Crossref | Google Scholar |

Graf DL, Cummings KS (2007) Review of the systematics and global diversity of freshwater mussel species (Bivalvia: Unionoida). Journal of Molluscan Studies 73(4), 291-314.
| Crossref | Google Scholar |

Graf DL, Cummings KS (2021) A ‘big data’ approach to global freshwater mussel diversity (Bivalvia: Unionoida), with an updated checklist of genera and species. Journal of Molluscan Studies 87(1), eyaa034.
| Crossref | Google Scholar |

Haag WR (2012) ‘North American freshwater mussels: natural history, ecology, and conservation.’ (Cambridge University Press: Cambridge, UK)

Haag WR, Williams JD (2013) Biodiversity on the brink: an assessment of conservation strategies for North American freshwater mussels. Hydrobiologia 735(1), 45-60.
| Crossref | Google Scholar |

Haas F (1969) ‘Superfamilia Unionacea.’ (Walter de Gruyter: Berlin, Germany) [In German]

He J, Zhuang Z (2013) ‘The Freshwater Bivalves of China.’ (ConchBooks: Harxheim, Germany)

Heude PM (1875) ‘Conchyliologie fluviatile de la province de Nanking et de la Chine centrale. Premier Fascicule.’ (Librairie F. Savy: Paris, France) [In French]

Heude PM (1877a) ‘Conchyliologie fluviatile de la province de Nanking et de la Chine centrale. Deuxième Fascicule.’ (Librairie F. Savy: Paris, France) [In French]

Heude PM (1877b) ‘Conchyliologie fluviatile de la province de Nanking et de la Chine centrale. Troisième Fascicule.’ (Librairie F. Savy: Paris, France) [In French]

Heude PM (1878) ‘Conchyliologie fluviatile de la province de Nanking et de la Chine centrale. Quatrième Fascicule.’ (Librairie F. Savy: Paris, France) [In French]

Heude PM (1879) ‘Conchyliologie fluviatile de la province de Nanking et de la Chine centrale. Cinquième Fascicule.’ (Librairie F. Savy: Paris, France) [In French]

Heude PM (1880a) ‘Conchyliologie fluviatile de la province de Nanking et de la Chine centrale. Sixième Fascicule.’ (Librairie F. Savy: Paris, France) [In French]

Heude PM (1880b) ‘Conchyliologie fluviatile de la province de Nanking et de la Chine centrale. Dixième Fascicule.’ (Librairie F. Savy: Paris, France) [In French]

Heude PM (1881) ‘Conchyliologie fluviatile de la province de Nanking et de la Chine centrale. Septième Fascicule.’ (Librairie F. Savy: Paris, France) [In French]

Heude PM (1883) ‘Conchyliologie fluviatile de la province de Nanking et de la Chine centrale. Huitième Fascicule.’ (Librairie F. Savy: Paris, France) [In French]

Heude PM (1885) ‘Conchyliologie fluviatile de la province de Nanking et de la Chine centrale. Neuvième Fascicule.’ (Librairie F. Savy: Paris, France) [In French]

Hewitt G (2000) The genetic legacy of the Quaternary ice ages. Nature 405(6789), 907-913.
| Crossref | Google Scholar | PubMed |

Huang XC, Wu RW, An CT, Xie GL, Su JH, Ouyang S, Zhou CH, Wu XP (2018) Reclassification of Lamprotula rochechouartii as Margaritifera rochechouartii comb. nov. (Bivalvia: Margaritiferidae) revealed by time-calibrated multi-locus phylogenetic analyses and mitochondrial phylogenomics of Unionoida. Molecular Phylogenetics and Evolution 120, 297-306.
| Crossref | Google Scholar | PubMed |

Huang XC, Su JH, Ouyang JX, Ouyang S, Zhou CH, Wu XP (2019) Towards a global phylogeny of freshwater mussels (Bivalvia: Unionida): species delimitation of Chinese taxa, mitochondrial phylogenomics, and diversification patterns. Molecular Phylogenetics and Evolution 130, 45-59.
| Crossref | Google Scholar | PubMed |

Hughes JM, Schmidt DJ, Finn DS (2009) Genes in streams: using DNA to understand the movement of freshwater fauna and their riverine habitat. BioScience 59(7), 573-583.
| Crossref | Google Scholar |

Inoue K, Harris JL, Robertson CR, Johnson NA, Randklev CR (2020) A comprehensive approach uncovers hidden diversity in freshwater mussels (Bivalvia: Unionidae) with the description of a novel species. Cladistics 36(1), 88-113.
| Crossref | Google Scholar | PubMed |

International Union for Conservation of Nature (2021) The IUCN Red List of Threatened Species. V.2018-3. Available at https://www.iucnredlist.org [Verified 10 September 2022]

Jeratthitikul E, Sucharit C, Prasankok P (2019) Molecular phylogeny of the Indochinese freshwater mussel genus Scabies Haas, 1911 (Bivalvia: Unionidae). Tropical Natural History 19(1), 21-36.
| Google Scholar |

Johnson NA, Smith CH, Pfeiffer JM, Randklev CR, Williams JD, Austin JD (2018) Integrative taxonomy resolves taxonomic uncertainty for freshwater mussels being considered for protection under the U.S. Endangered Species Act. Scientific Reports 8(1), 15892.
| Crossref | Google Scholar | PubMed |

Katoh K, Rozewicki J, Yamada KD (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20(4), 1160-1166.
| Crossref | Google Scholar | PubMed |

Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28(12), 1647-1649.
| Crossref | Google Scholar | PubMed |

Khan JM, Dudding J, Hart M, Tsakiris E, Randklev CR (2020) Linking life history strategies and historical baseline information shows effects of altered flow regimes and impoundments on freshwater mussel assemblages. Freshwater Biology 65(11), 1950-1961.
| Crossref | Google Scholar |

Kim KS, Roe KJ (2021) Genome-wide SNPs redefines species boundaries and conservation units in the freshwater mussel genus Cyprogenia of North America. Scientific Reports 11(1), 10752.
| Crossref | Google Scholar |

Klishko O, Lopes-Lima M, Froufe E, Bogan A, Vasiliev L, Yanovich L (2017) Taxonomic reassessment of the freshwater mussel genus Unio (Bivalvia: Unionidae) in Russia and Ukraine based on morphological and molecular data. Zootaxa 4286(1), 93-112.
| Crossref | Google Scholar |

Klishko OK, Lopes-Lima M, Froufe E, Bogan AE, Abakumova VY (2018) Unravelling the systematics of Nodularia (Bivalvia, Unionidae) species from eastern Russia. Systematics and Biodiversity 16(3), 287-301.
| Crossref | Google Scholar |

Konopleva ES, Bolotov IN, Vikhrev IV, Inkhavilay K, Gofarov MY, Kondakov AV, Tomilova AA, Chapurina YE, Van Do T, Pfeiffer JM, Lopes-Lima M, Bogan AE (2023) A freshwater mussel species reflects a Miocene stream capture between the Mekong Basin and East Asian rivers. Zoosystematics and Evolution 99(1), 29-43.
| Crossref | Google Scholar |

Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35(6), 1547-1549.
| Crossref | Google Scholar | PubMed |

Lanfear R, Frandsen PB, Wright AM, Senfeld T, Calcott B (2017) PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution 34(3), 772-773.
| Crossref | Google Scholar | PubMed |

Laslett D, Canbäck B (2008) ARWEN: a program to detect tRNA genes in metazoan mitochondrial nucleotide sequences. Bioinformatics 24(2), 172-175.
| Crossref | Google Scholar | PubMed |

Leigh JW, Bryant D, Nakagawa S (2015) POPART: full‐feature software for haplotype network construction. Methods in Ecology and Evolution 6(9), 1110-1116.
| Google Scholar |

Li X, Yang Y, Henry RJ, Rossetto M, Wang Y, Chen S (2015) Plant DNA barcoding: from gene to genome. Biological Reviews 90(1), 157-166.
| Crossref | Google Scholar | PubMed |

Liu XJ, Cao YL, Xue TT, Wu RW, Zhou Y, Zhou C, Zanatta DT, Ouyang S, Wu XP (2017) Genetic structure and diversity of Nodularia douglasiae (Bivalvia: Unionida) from the middle and lower Yangtze River drainage. PLoS ONE 12(12), e0189737.
| Crossref | Google Scholar |

Liu XJ, Liu YY, Wu RW, Zanatta DT, Lopes-Lima M, Duarte VG, Bogan AE, Ouyang S, Wu XP (2022) Systematics, distribution, biology, and conservation of freshwater mussels (Bivalvia: Unionida) in China. Aquatic Conservation: Marine and Freshwater Ecosystems 32(5), 859-895.
| Crossref | Google Scholar |

Liu YY, Duan YH (1991) 中国糙蚌属二新种(瓣鳃纲:蚌科)[Two new species of freshwater mussel of the genus Scabies from China.]. Acta Zootaxonomica Sinica 16(3), 278-281 [In Chinese].
| Google Scholar |

Liu YY, Zhang WZ, Wang YX, Wang EY (1979) 中国经济动物志—淡水软体动物 [‘Chinese Economic Zoology – Freshwater Molluscs.’] (Science Press: Beijing, PR China) [In Chinese]

Lopes-Lima M, Froufe E, Do VT, Ghamizi M, Mock KE, Kebapçı Ü, Klishko O, Kovitvadhi S, Kovitvadhi U, Paulo OS, Pfeiffer JM, Raley M, Riccardi N, Sereflisan H, Sousa R, Teixeira A, Varandas S, Wu XP, Zanatta DT, Zieritz A, Bogan AE (2017a) Phylogeny of the most species-rich freshwater bivalve family (Bivalvia: Unionida: Unionidae): defining modern subfamilies and tribes. Molecular Phylogenetics and Evolution 106, 174-191.
| Crossref | Google Scholar | PubMed |

Lopes-Lima M, Sousa R, Geist J, Aldridge DC, Araujo R, Bergengren J, Bespalaya Y, Bo´dis E, Burlakova L, Van Damme D, Douda K, Froufe E, Georgiev D, Gumpinger C, Karatayev A, Kebapçı Ü, Killeen I, Lajtner J, Larsen BM, Lauceri R, Legakis A, Lois S, Lundberg S, Moorkens E, Motte G, Nagel KO, Ondina P, Outeiro A, Paunovic M, Prié V, von Proschwitz T, Riccardi N, Rudzīte M, Rudzītis M, Scheder C, Seddon M, Şereflişan H, Simić V, Sokolova S, Stoeckl K, Taskinen J, Teixeira A, Thielen F, Trichkova T, Varandas S, Vicentini H, Zajac K, Zajac T, Zogaris S (2017b) Conservation status of freshwater mussels in Europe: state of the art and future challenges. Biological Reviews 92(1), 572-607.
| Crossref | Google Scholar | PubMed |

Lopes-Lima M, Burlakova LE, Karatayev AY, Mehler K, Seddon M, Sousa R (2018) Conservation of freshwater bivalves at the global scale: diversity, threats and research needs. Hydrobiologia 810(1), 1-14.
| Crossref | Google Scholar |

Lopes-Lima M, Hattori A, Kondo T, Hee Lee J, Ki Kim S, Shirai A, Hayashi H, Usui T, Sakuma K, Toriya T, Sunamura Y, Ishikawa H, Hoshino N, Kusano Y, Kumaki H, Utsugi Y, Yabe S, Yoshinari Y, Hiruma H, Tanaka A, Sao K, Ueda T, Sano I, Miyazaki JI, Gonçalves DV, Klishko OK, Konopleva ES, Vikhrev IV, Kondakov AV, Yu Gofarov M, Bolotov IN, Sayenko EM, Soroka M, Zieritz A, Bogan AE, Froufe E (2020) Freshwater mussels (Bivalvia: Unionidae) from the rising sun (Far East Asia): phylogeny, systematics, and distribution. Molecular Phylogenetics and Evolution 146, 106755.
| Crossref | Google Scholar | PubMed |

Lopes-Lima M, Gürlek ME, Kebapçı Ü, Şereflişan H, Yanık T, Mirzajani A, Neubert E, Prié V, Teixeira A, Gomes-Dos-Santos A, Barros-García D, Bolotov IN, Kondakov AV, Vikhrev IV, Tomilova AA, Özcan T, Altun A, Gonçalves DV, Bogan AE, Froufe E (2021a) Diversity, biogeography, evolutionary relationships, and conservation of Eastern Mediterranean freshwater mussels (Bivalvia: Unionidae). Molecular Phylogenetics and Evolution 163, 107261.
| Crossref | Google Scholar | PubMed |

Lopes-Lima M, Riccardi N, Urbanska M, Köhler F, Vinarski M, Bogan AE, Sousa R (2021b) Major shortfalls impairing knowledge and conservation of freshwater molluscs. Hydrobiologia 848(12), 2831-2867.
| Crossref | Google Scholar |

Mace GM (2004) The role of taxonomy in species conservation. Philosophical Transactions of the Royal Society of London – B. Biological Science 359(1444), 711-719.
| Crossref | Google Scholar | PubMed |

Neemuchwala S, Johnson NA, Pfeiffer JM, Lopes-Lima M, Gomes-dos-Santos A, Froufe E, Hillis DM, Smith CH (2023) Coevolution with host fishes shapes parasitic life histories in a group of freshwater mussels (Unionidae: Quadrulini). Bulletin of the Society of Systematic Biologists 2(1), 1-25.
| Crossref | Google Scholar |

Neves RJ, Moyer SN (1988) Evaluation of techniques for age determination of freshwater mussels (Unionidae). American Malacological Bulletin 6(2), 179-188.
| Google Scholar |

Park JK, Ó Foighil D (2000) Sphaeriid and corbiculid clams represent separate heterodont bivalve radiations into freshwater environments. Molecular Phylogenetics and Evolution 14(1), 75-88.
| Crossref | Google Scholar | PubMed |

Pfeiffer JM, Breinholt JW, Page LM (2019) Unioverse: a phylogenomic resource for reconstructing the evolution of freshwater mussels (Bivalvia, Unionoida). Molecular Phylogenetics and Evolution 137, 114-126.
| Crossref | Google Scholar | PubMed |

Pfeiffer JM, Graf DL, Cummings KS, Page LM (2021) Taxonomic revision of a radiation of South-east Asian freshwater mussels (Unionidae: Gonideinae: Contradentini + Rectidentini). Invertebrate Systematics 35(4), 394-470.
| Crossref | Google Scholar |

Puillandre N, Modica MV, Zhang Y, Sirovich L, Boisselier MC, Cruaud C, Holford M, Samadi S (2012) Large-scale species delimitation method for hyperdiverse groups. Molecular Ecology 21(11), 2671-2691.
| Crossref | Google Scholar | PubMed |

Randklev CR, Tsakris ET, Johnson MS, Popejoy T, Hart MA, Khan J, Geeslin D, Robertson CR (2018) The effect of dewatering on freshwater mussel (Unionidae) community structure and the implications for conservation and water policy: a case study from a spring-fed stream in the southwestern United States. Global Ecology and Conservation 16, e00456.
| Crossref | Google Scholar |

Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61(3), 539-542.
| Crossref | Google Scholar | PubMed |

Rozas J, Ferrer-Mata A, Sánchez-DelBarrio JC, Guirao-Rico S, Librado P, Ramos-Onsins SE, Sánchez-Gracia A (2017) DnaSP 6: DNA sequence polymorphism analysis of large data sets. Molecular Biology and Evolution 34(12), 3299-3302.
| Crossref | Google Scholar | PubMed |

Saxon R, Randklev CR, Roe KJ, Robertson CR, Smith CH (2023) A phylogeographic assessment redefines the distributions of Truncilla species (Bivalvia: Unionidae) in Texas. Journal of Molluscan Studies 89(3), eyad015.
| Crossref | Google Scholar |

Sayenko EM, Soroka M, Akiyama YB, Uechi T, Ito K, Kondo M (2020) Taxonomic status of genera Nodularia, Middendorffinaia and Inversiunio (Bivalvia: Unionidae) from South-East Asia: morphometric, genetic and GenBank data. Systematics and Biodiversity 19(1), 54-73.
| Crossref | Google Scholar |

Simpson CT (1900) ‘Synopsis of the Naiades: or pearly fresh-water mussels.’ (US Government Printing Office: Washington, DC, USA)doi:10.5962/bhl.title.935

Smith CH, Johnson NA, Inoue K, Doyle RD, Randklev CR (2019) Integrative taxonomy reveals a new species of freshwater mussel, Potamilus streckersoni sp. nov. (Bivalvia: Unionidae): implications for conservation and management. Systematics and Biodiversity 17(4), 331-348.
| Crossref | Google Scholar |

Smith CH, Johnson NA, Robertson CR, Doyle RD, Randklev CR (2021) Establishing conservation units to promote recovery of two threatened freshwater mussel species (Bivalvia: Unionida: Potamilus). Ecology and Evolution 11(16), 11102-11122.
| Crossref | Google Scholar | PubMed |

Vaughn CC (2018) Ecosystem services provided by freshwater mussels. Hydrobiologia 810(1), 15-27.
| Crossref | Google Scholar |

Walker JM, Bogan AE, Bonfiglio EA, Campbell DC, Christian AD, Curole JP, Harris JL, Wojtecki RJ, Hoeh WR (2007) Primers for amplifying the hypervariable, male-transmitted COII–COI junction region in amblemine freshwater mussels (Bivalvia: Unionoidea: Ambleminae). Molecular Ecology Notes 7(3), 489-491.
| Crossref | Google Scholar |

Wang G, Wu C, Ge J, Chen Y, Han Z, Guo P, Li J (2019) Identification of complete F-type mitochondrial genome in Lamprotula scripta and Lamprotula caveata and analysis on DUI. Gene 710, 59-65.
| Crossref | Google Scholar | PubMed |

Wu RW, Liu YT, Wang S, Liu XJ, Zanatta DT, Roe KJ, Song XL, An CT, Wu XP (2018) Testing the utility of DNA barcodes and a preliminary phylogenetic framework for Chinese freshwater mussels (Bivalvia: Unionidae) from the middle and lower Yangtze River. PLoS One 13(8), e0200956.
| Crossref | Google Scholar |

Wu RW, Liu XJ, Wang S, Roe KJ, Ouyang S, Wu XP (2019) Analysis of mitochondrial genomes resolves the phylogenetic position of Chinese freshwater mussels (Bivalvia, Unionidae). ZooKeys 812, 23-46.
| Crossref | Google Scholar | PubMed |

Wu XP, Dai YT, Yin N, Shu FY, Chen ZG, Guo L, Zhou CH, Ouyang S, Huang XC (2022) Mitogenomic phylogeny resolves Cuneopsis (Bivalvia: Unionidae) as polyphyletic: the description of two new genera and a new species. Zoologica Scripta 51(2), 173-184.
| Crossref | Google Scholar |

Yu XQ, Jiang YZ, Folk RA, Zhao JL, Fu CN, Fang L, Peng H, Yang JB, Yang SX (2022) Species discrimination in Schima (Theaceae): next-generation super-barcodes meet evolutionary complexity. Molecular Ecology Resources 22(8), 3161-3175.
| Crossref | Google Scholar | PubMed |

Zhang J, Kapli P, Pavlidis P, Stamatakis A (2013) A general species delimitation method with applications to phylogenetic placements. Bioinformatics 29(22), 2869-2876.
| Crossref | Google Scholar | PubMed |

Zheng S, Poczai P, Hyvönen J, Tang J, Amiryousefi A (2020) Chloroplot: an online program for the versatile plotting of organelle genomes. Frontiers in Genetics 11, 576124.
| Crossref | Google Scholar |

Zieritz A, Aldridge DC (2009) Identification of ecophenotypic trends within three European freshwater mussel species (Bivalvia: Unionoida) using traditional and modern morphometric techniques. Biological Journal of the Linnean Society 98(4), 814-825.
| Crossref | Google Scholar |

Zieritz A, Bogan AE, Froufe E, Klishko O, Kondo T, Kovitvadhi U, Kovitvadhi S, Lee JH, Lopes-Lima M, Pfeiffer JM, Sousa R, Van Do T, Vikhrev I, Zanatta DT (2018) Diversity, biogeography and conservation of freshwater mussels (Bivalvia: Unionida) in East and Southeast Asia. Hydrobiologia 810, 29-44.
| Crossref | Google Scholar |

Zieritz A, Froufe E, Bolotov I, Gonçalves DV, Aldridge DC, Bogan AE, Gan HM, Gomes-Dos-Santos A, Sousa R, Teixeira A, Varandas S, Zanatta D, Lopes-Lima M (2021) Mitogenomic phylogeny and fossil-calibrated mutation rates for all F- and M-type mtDNA genes of the largest freshwater mussel family, the Unionidae (Bivalvia). Zoological Journal of the Linnean Society 193(3), 1088-1107.
| Crossref | Google Scholar |