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Invertebrate Systematics Invertebrate Systematics Society
Systematics, phylogeny and biogeography
RESEARCH ARTICLE

Integrative taxonomy of Teucriogethes pollen beetles (Coleoptera: Nitidulidae: Meligethinae), with implications on the systematics of the genus Teucrium (Lamiaceae)

Meike Liu https://orcid.org/0000-0001-8206-0936 A * , Qiuhong Li https://orcid.org/0009-0002-0320-6322 B , Pietro Gardini https://orcid.org/0000-0002-6308-709X C , Paolo Audisio C and Simone Sabatelli C
+ Author Affiliations
- Author Affiliations

A Institute of Entomology, College of Agriculture, Yangtze University, Jingzhou, Hubei, PR China.

B MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou, Hubei, PR China. Email: liqiuhong0309@163.com

C Department of Biology and Biotechnologies “Charles Darwin”, Sapienza University of Rome, Viale dell’Università 32, I-00185 Rome, Italy. Email: pietro.gardini@uniroma1.it; paolo.audisio@uniroma1.it; simone.sabatelli@uniroma1.it

* Correspondence to: liumk2009@126.com

Handling Editor: Bruno Medeiros

Invertebrate Systematics 38, IS23025 https://doi.org/10.1071/IS23025
Submitted: 25 May 2023  Accepted: 13 September 2024  Published: 11 October 2024

© 2024 The Author(s) (or their employer(s)). Published by CSIRO Publishing.

Abstract

In highly specialised phytophagous insects, important insights on phylogeny of the involved insect group can be often inferred from the evolutionary history of their host plants, and vice-versa, as in the case for a new, peculiar species of pollen beetles, recently discovered in Central China (Hubei Province). This new species is described herein as Teucriogethes huangae Liu & Audisio, sp. nov., and information on its ecology and systematic position is provided. The new species, associated as larvae with a strongly isolated member of the genus Teucrium L. (Lamiaceae), T. ornatum Hemsl., exhibiting a problematic taxonomic position, represents in turn an isolated and morphologically rather aberrant taxon in its lineage, probably the largest in size (2.2–2.8 mm), and the first endemic representative of its genus in the whole Eastern Palaearctic. An updating of the taxonomic assessment of members of Teucriogethes and on possible evolutionary relationships with their larval host plants, based on an integrative taxonomy approach, is finally presented. A key to identification of all known species is also introduced.

ZooBank: urn:lsid:zoobank.org:pub:E349044B-29A5-416C-85CA-56143C7A29BE

Keywords: China, systematics, new species, identification key, host plants, parallel evolution, molecular phylogeny, pollen beetles.

References

Abu-Asab MS, Cantino PD (1993) Phylogenetic implications of pollen morphology in tribe Ajugeae (Labiatae). Systematic Botany 18, 100-122.
| Crossref | Google Scholar |

Aljanabi SM, Martinez I (1997) Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acid Research 25, 4692-4693.
| Crossref | Google Scholar | PubMed |

Audisio P (1993) ‘Coleoptera, Nitidulidae – Kateretidae. Fauna d’Italia: 32.’ (Edizioni Calderini: Bologna, Italy) [In Italian, with Italian and English keys]

Audisio P, Jelínek J (1990) Tassonomia e distribuzione geografica di Meligethes obscurus Auct., con descrizione di una specie nuova (Coleoptera, Nitidulidae). Fragmenta Entomologica 22, 75-85 [In Italian].
| Google Scholar |

Audisio P, Jelínek J, Cooter J (2005) New and little-known species of Meligethes Stephens, 1830 from China (Coleoptera: Nitidulidae). Acta Entomologica Musei Nationalis Pragae 45, 111-127.
| Google Scholar |

Audisio P, Cline AR, De Biase A, Antonini G, Mancini E, Trizzino M, Costantini L, Strika S, Lamanna F, Cerretti P (2009a) Preliminary re-examination of genus-level taxonomy of the pollen beetle subfamily Meligethinae (Coleoptera: Nitidulidae). Acta Entomologica Musei Nationalis Pragae 49, 341-504.
| Google Scholar |

Audisio P, Cline AR, Lamanna F, Trizzino M, Antonini G, Mancini E, De Biase A (2009b) Revision of the southern African pollen beetle genus Anthystrix (Coleoptera: Nitidulidae: Meligethinae). Annals of the Entomological Society of America 102, 998-1012.
| Crossref | Google Scholar |

Audisio P, Sabatelli S, Jelínek J (2015a) Revision of the pollen beetle genus Meligethes (Coleoptera: Nitidulidae). Fragmenta Entomologica 46, 19-112.
| Crossref | Google Scholar |

Audisio P, Cline AR, Trizzino M, Mancini E, Antonini G, Sabatelli S, Cerretti P (2015b) Revision of the African pollen beetle genera Tarchonanthogethes and Xenostrongylogethes, with insect–host plant relationships, identification key, and cladistic analysis of the Anthystrix genus complex (Coleoptera: Nitidulidae: Meligethinae). Zootaxa 3920(1), 101-152.
| Crossref | Google Scholar |

Audisio P, Cline AR, Lason A, Jelínek J, Sabatelli S, Serri S (2017) New species and records of pollen and sap beetles for Iran (Coleoptera: Kateretidae, Nitidulidae). Zootaxa 4216, 369-383.
| Crossref | Google Scholar | PubMed |

Chen Y, Lin XL, Huang M, Yang XK (2015) A new species of Lamiogethes and a new species of Meligethes from China (Coleoptera: Nitidulidae: Meligethinae). Zootaxa 3999, 413-420.
| Crossref | Google Scholar | PubMed |

Cline AR, Smith TR, Miller K, Moulton M, Whiting M, Audisio P (2014) Molecular phylogeny of Nitidulidae: assessment of subfamilial and tribal classification and formalization of the family Cybocephalidae (Coleoptera: Cucujoidea). Systematic Entomology 39, 758-772.
| Crossref | Google Scholar |

Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial Cytochrome C oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294-299.
| Google Scholar | PubMed |

Harley RM, Atkins S, Budantsev AL, Cantino PD, Conn BJ, Grayer R, Harley MM, De Kok R, Krestovskaja T, Morales R, Paton AJ, Ryding O, Upson T (2004) Labiatae. The Families and Genera of Vascular Plants 7, 196-203.
| Crossref | Google Scholar |

Jelínek J, Audisio P (2007) Family Nitidulidae. In ‘Catalogue of Palaearctic Coleoptera. Vol. 4. Elateroidea – Derodontoidea – Bostrichoidea – Lymexyloidea – Cleroidea – Cucujoidea’. (Eds I Lobl, A Smetana) pp. 459–491. (Apollo Books: Stenstrup, Denmark)

Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30, 772-780.
| Crossref | Google Scholar | PubMed |

Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28, 1647-1649.
| Crossref | Google Scholar | PubMed |

Kirejtshuk AG (1992) Определительная таблица надсемейств Cucujoidea и Tenebrionoidea. Определительная таблица семейств надсемейства Cucujoidea. Сем. Блестянки – Nitidulidae. Сем. Катеретиды – Kateretidae [Key to the superfamilies Cucujoidea and Tenebrionoidea. Key to the families of the superfamily Cucujoidea. Family Nitidulidae. Family Kateretidae]. In Определитель насекомых Дальнего Востока [‘Key to insects of the Far East’]. (Ed. PA Ler) pp. 114–210. (Nauka: Saint Petersburg, Russian Federation) [In Russian]

Lewis PO (2001) A likelihood approach to estimating phylogeny from discrete morphological character data. Systematic Biology 50, 913-925.
| Crossref | Google Scholar | PubMed |

Li B, Cantino PD, Olmstead RG, Bramley GLC, Xiang CL, Ma ZH, Tan YH, Zhang DX (2016) A large-scale chloroplast phylogeny of the Lamiaceae sheds new light on its subfamilial classification. Scientific Reports 6, 34343.
| Crossref | Google Scholar | PubMed |

Li HW, Hedge IC (1994) Lamiaceae. In ‘Flora of China vol. 17’. (Eds CY Wu, PH Raven) pp. 269–291. (Science Press: Beijing, PR China; and Missouri Botanical Garden Press: Saint Louis, MO, USA)

Liu M, Yang X, Huang M, Jelínek J, Audisio P (2016) Four new species of Meligethes Stephens from China and additional data on other species of the genus (Coleoptera: Nitidulidae: Meligethinae). Zootaxa 4121, 101-116.
| Crossref | Google Scholar | PubMed |

Liu M, Huang M, Cline AR, Audisio P (2017a) Two new Lamiogethes Audisio & Cline from China (Coleoptera: Nitidulidae, Meligethinae). Fragmenta Entomologica 49, 145-150.
| Crossref | Google Scholar |

Liu M, Huang M, Cline AR, Sabatelli S, Audisio P (2017b) A new species of Meligethes Stephens from China and additional data on members of the M. chinensis species complex (Coleoptera: Nitidulidae, Meligethinae). Fragmenta Entomologica 49, 79-84.
| Crossref | Google Scholar |

Liu M, Huang M, Cline AR, Audisio P (2018) New and poorly known Meligethes Stephens from China, with bionomical data on some species (Coleoptera: Nitidulidae: Meligethinae). Zootaxa 4392, 546-566.
| Crossref | Google Scholar | PubMed |

Liu M, Sabatelli S, Mancini E, Trizzino M, Huang M, Cline AR, Audisio P (2019) Rediscovery of Brassicogethes salvan (Coleoptera: Nitidulidae, Meligethinae) in the southwestern Alps. Insect Conservation and Diversity 12(1), 80-87.
| Crossref | Google Scholar |

Liu M, Huang M, Cline AR, Cardoli P, Audisio P, Sabatelli S (2020a) Re-examination of the genus-level taxonomy of the pollen beetle subfamily Meligethinae – part 1. Sagittogethes Audisio & Cline, 2009 and allied genera; with description of a new genus (Coleoptera: Nitidulidae). Fragmenta Entomologica 52, 119-135.
| Crossref | Google Scholar |

Liu M, Yang X, Huang M, Cline AR, Sabatelli S, Audisio P (2020b) Five new species of Lamiogethes Audisio & Cline from China (Coleoptera: Nitidulidae: Meligethinae). Zootaxa 4728, 63-76.
| Crossref | Google Scholar |

Liu M, Huang M, Cline AR, Mancini E, Scaramuzzi A, Paradisi S, Audisio P, Badano D, Sabatelli S (2021) Rosaceae, Brassicaceae and pollen beetles: exploring relationships and evolution in an anthophilous beetle lineage (Nitidulidae, Meligethes complex of genera) using an integrative approach. Frontiers in Zoology 18, 9.
| Crossref | Google Scholar | PubMed |

Minh BQ, Nguyen MAT, Von Haeseler A (2013) Ultrafast approximation for phylogenetic bootstrap. Molecular Biology and Evolution 30, 1188-1195.
| Crossref | Google Scholar | PubMed |

Navarro T (2020) Systematics and biogeography of the genus Teucrium (Lamiaceae). In ‘Biology and Applications. Vol. 39’. (Ed. M Stanković) pp. 1–38. (Springer) 10.1007/978-3-030-52159-2_1

Navarro T, EI Oualidi J (2000) Synopsis of Teucrium L. (Lamiaceae) in the Mediterranean region and surrounding areas. Flora Mediterranea 10, 349-363.
| Google Scholar |

Nguyen LT, Schmidt HA, von Haeseler A, Minh BQ (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution 32, 268-274.
| Crossref | Google Scholar | PubMed |

Posada D (2008) jModelTest: phylogenetic model averaging. Molecular Phylogenetic & Evolution 25, 1253-1256.
| Crossref | Google Scholar | PubMed |

Rambaut A, Drummond AJ, Xie D, Baele G, Suchard MA (2018) Posterior summarization in Bayesian phylogenetics using Tracer 1.7. Systematic Biology 67, 901-904.
| Crossref | Google Scholar | PubMed |

Ranjbar M, Mahmoudi C, Naari H (2018) An overview of chromosomal criteria and biogeography in the genus Teucrium (Lamiaceae). Caryologia 71, 63-79.
| Crossref | Google Scholar |

Ribera I, Fresneda J, Bucur R, Izquierdo A, Vogler AP, Salgado JM, Cieslak A (2010) Ancient origin of a Western Mediterranean radiation of subterranean beetles. BMC Evolutionary Biology 10, 29.
| Crossref | Google Scholar | PubMed |

Rodríguez F, Oliver JL, Marín A, Medina JR (1990) The general stochastic model of nucleotide substitution. Journal of Theoretical Biology 142, 485-501.
| Crossref | Google Scholar | PubMed |

Ronquist F, Teslenko M, Van Der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61, 539-542.
| Crossref | Google Scholar | PubMed |

Sabatelli S, Liu M, Badano D, Mancini E, Trizzino M, Cline AR, Endrestøl A, Huang M, Audisio P (2020) Molecular phylogeny and host-plant use (Lamiaceae) of the Thymogethes pollen beetles (Coleoptera). Zoologica Scripta 49, 28-46.
| Crossref | Google Scholar |

Sabatelli S, Ruspantini P, Cardoli P, Audisio P (2021) Underestimated diversity: cryptic species and phylogenetic relationships in the subgenus Cobalius (Coleoptera: Hydraenidae) from marine rockpools. Molecular Phylogenetics and Evolution 163, 107243.
| Crossref | Google Scholar | PubMed |

Salmaki Y, Kattari S, Heubl G, Bräuchler C (2016) Phylogeny of non-monophyletic Teucrium (Lamiaceae: Ajugoideae): implications for character evolution and taxonomy. Taxon 65, 805-822.
| Crossref | Google Scholar |

Simon C, Frati F, Beckenbach A, Crespi B, Liu H, Flook P (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America 87, 651-701.
| Crossref | Google Scholar |

Stanković M (2020) ‘Teucrium Species: Biology and Applications.’ (Springer Nature Switzerland AG: Cham, Switzerland) 10.1007/978-3-030-52159-2

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30, 2725-2729.
| Crossref | Google Scholar | PubMed |

Trifinopoulos J, Nguyen LT, Von Haeseler ABQ (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acid Research 44(W1), W232-W235.
| Crossref | Google Scholar | PubMed |

Trizzino M, Audisio P, Antonini G, De Biase A, Mancini E (2009) Comparative analysis of sequences and secondary structures of the rRNA internal transcribed spacer 2 (ITS2) in pollen-beetles of the subfamily Meligethinae (Coleoptera, Nitidulidae): potential use of slippage-derived sequences in molecular systematics. Molecular Phylogenetics and Evolution 51, 215-226.
| Crossref | Google Scholar | PubMed |

Wild AL, Maddison DR (2008) Evaluating nuclear protein-coding genes for phylogenetic utility in beetles. Molecular Phylogenetics and Evolution 48, 877-891.
| Crossref | Google Scholar | PubMed |

Xiang CL, Zhao F, Cantino PD, Drew BT, Li B, Liu ED, Soltis DE, Soltis PS, Peng H (2018) Molecular systematics of Caryopteris (Lamiaceae) and its allies with reference to the molecular phylogeny of subfamily Ajugoideae. Taxon 67, 376-394.
| Crossref | Google Scholar |