High species turnover of the ant genus Solenopsis (Hymenoptera : Formicidae) along an altitudinal gradient in the Ecuadorian Andes, indicated by a combined DNA sequencing and morphological approach
Thibaut Delsinne A C , Gontran Sonet B , Zoltán T. Nagy B , Nina Wauters A , Justine Jacquemin A and Maurice Leponce A
+ Author Affiliations
- Author Affiliations
A Biological Evaluation Section, Royal Belgian Institute of Natural Sciences, Rue Vautier 29, B-1000 Brussels, Belgium.
B Joint Experimental Molecular Unit, Royal Belgian Institute of Natural Sciences, Rue Vautier 29, B-1000 Brussels, Belgium.
C Corresponding author. Email: Thibaut.Delsinne@sciencesnaturelles.be
Invertebrate Systematics 26(6) 457-469 https://doi.org/10.1071/IS12030
Submitted: 18 April 2012 Accepted: 16 September 2012 Published: 19 December 2012
Abstract
Solenopsis is a widespread ant genus and the identification of its species is notoriously difficult. Hence, investigation of their distribution along elevational gradients is challenging. Our aims were (1) to test the complementarity of the morphological and DNA barcoding approaches for Solenopsis species identification, and (2) to assess species diversity and distribution along an altitudinal gradient in the Ecuadorian Andes. Ants were collected in five localities between 1000 and 3000 m above sea level. In total, 24 morphospecies were identified along the gradient and 14 of them were barcoded. Seven morphospecies were confirmed by the molecular approach. Three others, occurring sympatrically and possessing clear diagnostic characters, showed low genetic divergence. Representatives of a further four morphospecies were split into nine clusters by COI and nuclear wingless genetic markers, suggesting the existence of cryptic species. Examination of gynes revealed potential diagnostic characters for morphological discrimination. Solenopsis species were found up to an altitudinal record of 3000 m. Most morphospecies (20 of 24) were found at a single elevation. Our results suggest a high species turnover along the gradient, and point to the use of morphological and DNA barcoding approaches as necessary for differentiating among Solenopsis species.
References
Abouheif, E., and Wray, G. A. (2002). Evolution of the gene network underlying wing polyphenism in ants. Science 297, 249–252.| Evolution of the gene network underlying wing polyphenism in ants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XlsVCnsb4%3D&md5=9e53ae85c7a93a809d500b09a3e46af5CAS |
Beck, E., Makeschin, F., Haubrich, M., Richter, M., Bendix, J., and Valerezo, C. (2008). The Ecosystem (Reserva Biológica San Francisco). In ‘Gradients in a Tropical Mountain Ecosystem of Ecuador’. (Eds E. Beck, J. Bendix, I. Kottke, F. Makeschin, and R. Mosandl.) pp 1–13. Ecological Studies 198. (Springer: Berlin & Heidelberg.) , .
| The Ecosystem (Reserva Biológica San Francisco). In ‘Gradients in a Tropical Mountain Ecosystem of Ecuador’. (Eds E. Beck, J. Bendix, I. Kottke, F. Makeschin, and R. Mosandl.) pp 1–13. Ecological Studies 198. (Springer: Berlin & Heidelberg.)Crossref | GoogleScholarGoogle Scholar |
Blüthgen, N., Verhaagh, M., Goitía, W., Jaffé, K., Morawetz, W., and Barthlott, W. (2000). How plants shape the ant community in the Amazonian rainforest canopy: the key role of extrafloral nectaries. Oecologia 125, 229–240.
| How plants shape the ant community in the Amazonian rainforest canopy: the key role of extrafloral nectaries.Crossref | GoogleScholarGoogle Scholar |
Bolton, B. (2003). Synopsis and classification of Formicidae. Memoirs of the American Entomological Institute 71, 1–370.
Bolton, B. (2012). AntCat. An online catalog of the ants of the world. www.antcat.org. [Accessed on 14 April 2012].
Braga, D., Louzada, J., Zanetti, R., and Delabie, J. (2010). Avaliação rápida da diversidade de formigas em sistemas de uso do solo no sul da Bahia. Neotropical Entomology 39, 464–469.
| Avaliação rápida da diversidade de formigas em sistemas de uso do solo no sul da Bahia.Crossref | GoogleScholarGoogle Scholar |
Brehm, G., Homeier, J., Fiedler, K., Kottke, I., Illig, J., Nöske, N. M., Werner, F. A., and Breckle, S.-W. (2008). Mountain rain forests in southern Ecuador as a hotspot of biodiversity – limited knowledge and diverging patterns. In ‘Gradients in a Tropical Mountain Ecosystem of Ecuador’. (Eds E. Beck, J. Bendix, I Kottke, F. Makeschin, and R. Mosandl.) pp. 15–23. Ecological Studies 198. (Springer: Berlin & Heidelberg.)
Brown, S. D., Collins, R. A., Boyer, S., Lefort, M. C., Malumbres-Olarte, J., Vink, C. J., and Cruickshank, R. H. (2012). Spider: An R package for the analysis of species identity and evolution, with particular reference to DNA barcoding. Molecular Ecology Resources 12, 562–565.
| Spider: An R package for the analysis of species identity and evolution, with particular reference to DNA barcoding.Crossref | GoogleScholarGoogle Scholar |
Burns, J. M., Janzen, D. H., Hajibabaei, M., Hallwachs, W., and Hebert, P. D. N. (2007). DNA barcodes of closely related (but morphologically and ecologically distinct) species of skipper butterflies (Hesperiidae) can differ by only one to three nucleotides. Journal of The Lepidopterists’ Society 61, 138–153.
Burns, J. M., Janzen, D. H., Hajibabaei, M., Hallwachs, W., and Hebert, P. D. N. (2008). DNA barcodes and cryptic species of skipper butterflies in the genus Perichares in Area de Conservación Guanacaste, Costa Rica. Proceedings of the National Academy of Sciences of the United States of America 105, 6350–6355.
| DNA barcodes and cryptic species of skipper butterflies in the genus Perichares in Area de Conservación Guanacaste, Costa Rica.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXlsFyhtLY%3D&md5=f8e56846152c0c4b83f5d6fbaa224e08CAS |
Creighton, W. S. (1930). The New World species of the genus Solenopsis (Hymenop. Formicidae) . Proceedings of the American Academy of Arts and Sciences 66, 39–152.
| The New World species of the genus Solenopsis (Hymenop. Formicidae) .Crossref | GoogleScholarGoogle Scholar |
Creighton, W. S. (1950). The ants of North America. Bulletin of the Museum of Comparative Zoology at Harvard College. The Cosmos Press, Inc., Cambridge, Mass., USA 104, 1–585.
Donoso, D. A., and Ramón, G. (2009). Composition of a high diversity leaf litter ant community (Hymenoptera: Formicidae) from an Ecuadorian pre-montane rainforest. Annales de la Société Entomologique de France 45, 487–499.
Dunn, R. R., Guénard, B., Weiser, M. D., and Sanders, N. J. (2010). Geographic gradients. In ‘Ant Ecology’. (Eds L. Lach, C. Parr, and K. Abbot.) pp. 38–58. (Oxford University Press Inc., New York.)
Elias, M., Hill, R. I., Willmott, K. R., Dasmahapatra, K. K., Brower, A. V. Z., Mallet, J., and Jiggins, C. D. (2007). Limited performance of DNA barcoding in a diverse community of tropical butterflies. Proceedings of the Royal Society of London. Series B. Biological Sciences 274, 2881–2889.
| Limited performance of DNA barcoding in a diverse community of tropical butterflies.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtlOhtr%2FF&md5=e4e4d5ee88385893dc50d4d751b8298cCAS |
Ettershank, G. (1966). A generic revision of the World Myrmicinae related to Solenopsis and Pheidologeton (Hymenoptera: Formicidae). Australian Journal of Zoology 14, 73–171.
| A generic revision of the World Myrmicinae related to Solenopsis and Pheidologeton (Hymenoptera: Formicidae).Crossref | GoogleScholarGoogle Scholar |
Fernández, F., and Sendoya, S. (2004). List of Neotropical ants (Hymenoptera: Formicidae). Biota Colombiana 5, 3–93.
Fisher, B. L., and Smith, M. A. (2008). A revision of Malagasy species of Anochetus Mayr and Odontomachus Latreille (Hymenoptera: Formicidae). PLoS ONE 3, e1787.
| A revision of Malagasy species of Anochetus Mayr and Odontomachus Latreille (Hymenoptera: Formicidae).Crossref | GoogleScholarGoogle Scholar |
Folmer, O., Black, M., Hoeh, W., Lutz, R., and Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome C oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294–299.
| 1:CAS:528:DyaK2MXjt12gtLs%3D&md5=0a6e1d841a9ef8fafdf936427c59141bCAS |
Groc, S., Orivel, J., Dejean, A., Martin, J.-M., Etienne, M.-P., Corbara, B., and Delabie, J. H. C. (2009). Baseline study of the leaf-litter ant fauna in a French Guianese forest. Insect Conservation and Diversity 2, 183–193.
| Baseline study of the leaf-litter ant fauna in a French Guianese forest.Crossref | GoogleScholarGoogle Scholar |
Guénard, B., Weiser, M. D., and Dunn, R. R. (2010). Ant genera of the world. http://www.antmacroecology.org/ant_genera/Solenopsis.html. [Accessed on 13 April 2012.]
Hadley, A. (2010). CombineZp. Available from: http://www.hadleyweb.pwp.blueyonder.co.uk/CZP/News.htm [Accessed 6 June 2010].
Hebert, P. D. N., Cywinska, A., Ball, S. L., and deWaard, J. R. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B. Biological Sciences 270, 313–321.
| Biological identifications through DNA barcodes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXktVWiu7g%3D&md5=bacde07336013937477a1e8839a85156CAS |
Hebert, P. D. N., Penton, E. H., Burns, J. M., Janzen, D. H., and Hallwachs, W. (2004a). Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences of the United States of America 101, 14 812–14 817.
| Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXovVyju7g%3D&md5=0ae4f5812affc4879a365a936524c001CAS |
Hebert, P. D. N., Stoeckle, M. Y., Zemlak, T. S., and Francis, C. M. (2004b). Identification of birds through DNA barcodes. PLoS Biology 2, e312.
| Identification of birds through DNA barcodes.Crossref | GoogleScholarGoogle Scholar |
Herrera, H. W., and Causton, C. E. (2008). Distribution of fire ants Solenopsis geminata and Wasmannia auropunctata (Hymenoptera: Formicidae) in the Galapagos Islands. Galapagos Research 65, 11–14.
Jansen, G., Savolainen, R., and Vepsäläinen, K. (2009). DNA barcoding as a heuristic tool for classifying undescribed Nearctic Myrmica ants (Hymenoptera: Formicidae). Zoologica Scripta 38, 527–536.
| DNA barcoding as a heuristic tool for classifying undescribed Nearctic Myrmica ants (Hymenoptera: Formicidae).Crossref | GoogleScholarGoogle Scholar |
Janzen, D. H., Hallwachs, W., Blandin, P., Burns, J. M., Cadiou, J.-M., Chacon, I., Dapkey, T., Deans, A. R., Epstein, M. E., Espinoza, B., Franclemont, J. G., Haber, W. A., Hajibabaei, M., Hall, J. P. W., Hebert, P. D. N., Gauld, I. D., Harvey, D. J., Hausmann, A., Kitching, I. J., Lafontaine, D., Landry, J.-F., Lemaire, C., Miller, J. Y., Miller, J. S., Miller, L., Miller, S. E., Montero, J., Munroe, E., Rab Green, S., Ratnasingham, S., Rawlins, J. E., Robbins, R. K., Rodriguez, J. J., Rougerie, R., Sharkey, M. J., Smith, A. M., Solis, A. M., Sullivan, B. J., Thiaucourt, P., Wahl, D. B., Weller, S. J., Whitfield, J. B., Willmott, K. R., Wood, M. D., Woodley, N. E., and Wilson, J. J. (2009). Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity. Molecular Ecology Resources 9, 1–26.
| Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXlslOjsb4%3D&md5=b0599c80ef67fa9a139acbb7f2bd9c6aCAS |
Jinbo, U., Kato, T., and Ito, M. (2011). Current progress in DNA barcoding and future implications for entomology. Entomological Science 14, 107–124.
| Current progress in DNA barcoding and future implications for entomology.Crossref | GoogleScholarGoogle Scholar |
Lattke, J. E. (2003). Biogeografía de las hormigas neotropicales. In ‘Introducción a las hormigas de la región Neotropical’. (Ed. F. Fernández.) pp. 65–85. (Instituto de Investigación de Recursos Biológicos Alexander von Humboldt: Bogotá, Colombia.)
Liedloff, A. C. (1999). Mantel Nonparametric Test Calculator. Version 2.0. School of Natural Resource Sciences, Queensland University of Technology, Australia.
Mackay, W., and Mackay, E. (2002). ‘The Ants of New Mexico (Hymenoptera: Formicidae).’ (The Edwin Mellen Press: Lewiston, NY.)
Menke, S. B., Booth, W., Dunn, R. R., Schal, C., Vargo, E. L., and Silverman, J. (2010). Is it easy to be urban? Convergent success in urban habitats among lineages of a widespread native ant. PLoS ONE 5, e9194.
| Is it easy to be urban? Convergent success in urban habitats among lineages of a widespread native ant.Crossref | GoogleScholarGoogle Scholar |
Oliveira, P. Y., Souza, J. L. P., Baccaro, F. B., and Franklin, E. (2009). Ant species distribution along a topographic gradient in a “terra-firme” forest reserve in Central Amazonia. Pesquisa Agropecuária Brasileira 44, 852–860.
| Ant species distribution along a topographic gradient in a “terra-firme” forest reserve in Central Amazonia.Crossref | GoogleScholarGoogle Scholar |
Pacheco, J. A. (2007). The New World thief ants of the genus Solenopsis (Hymenoptera: Formicidae). Ph.D. Thesis. University of Texas at El Paso, Texas.
Pacheco, J., Herrera, H., and Mackay, W. (2007). A new species of thief ant of the genus Solenopsis from the Galápagos Islands (Hymenoptera: Formicidae). Sociobiology 50, 1075–1086.
Paradis, E. (2010). PEGAS: an R package for population genetics with an integrated-modular approach. Bioinformatics (Oxford, England) 26, 419–420.
| PEGAS: an R package for population genetics with an integrated-modular approach.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhs1Onsrs%3D&md5=875aad9a4a320687c5a5461d58e1ffbaCAS |
Paradis, E., Claude, J., and Strimmer, K. (2004). APE: analyses of phylogenetics and evolution in R language Bioinformatics (Oxford, England) 20, 289–290.
| APE: analyses of phylogenetics and evolution in R languageCrossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXms1eitg%3D%3D&md5=88cbeb6097b47dc80ffdfdd504bf1d77CAS |
Pitts, J. P., McHugh, J. V., and Ross, K. G. (2005). Cladistic analysis of the fire ants of the Solenopsis saevissima species-group (Hymenoptera: Formicidae). Zoologica Scripta 34, 493–505.
| Cladistic analysis of the fire ants of the Solenopsis saevissima species-group (Hymenoptera: Formicidae).Crossref | GoogleScholarGoogle Scholar |
Posada, D. (2008). JModelTest: phylogenetic model averaging. Molecular Biology and Evolution 25, 1253–1256.
| JModelTest: phylogenetic model averaging.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXotlKgsb4%3D&md5=8a1f07129ca906c4b292e237699ed9deCAS |
Rambaut, A., and Drummond, A. J. (2009). Tracer version 1.5. Computer program and documentation distributed by the author, website http://tree.bio.ed.ac.uk/software/tracer/. [Accessed April 2012.]
Ratnasingham, S., and Hebert, P. D. N. (2007). BOLD: The Barcode of Life Data System (www.barcodinglife.org). Molecular Ecology Notes 7, 355–364.
| BOLD: The Barcode of Life Data System (www.barcodinglife.org).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXntVyksbc%3D&md5=53cd40598900a88b3cc1c91ad7b9427cCAS |
Ronquist, F., and Huelsenbeck, J. P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics (Oxford, England) 19, 1572–1574.
| MrBayes 3: Bayesian phylogenetic inference under mixed models.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXntlKms7k%3D&md5=9c53a6e1009ac0a4829f70ecd07199e1CAS |
Ross, K. G., and Shoemaker, D. D. (2005). Species delimitation in native South American fire ants. Molecular Ecology 14, 3419–3438.
| Species delimitation in native South American fire ants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtFGitbjO&md5=55310ca4a42d8fad4695d73f6d520772CAS |
Ross, K. G., Gotzek, D., Ascunce, M. S., and Shoemaker, D. D. (2010). Species delimitation: a case study in a problematic taxon. Systematic Biology 59, 162–184.
| Species delimitation: a case study in a problematic taxon.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXis1aksrY%3D&md5=e51a651c69219fa7f31ec0d96f4008f3CAS |
Rubinoff, D., Cameron, S., and Will, K. (2006). A genomic perspective on the shortcomings of mitochondrial DNA for “barcoding” identification. The Journal of Heredity 97, 581–594.
| A genomic perspective on the shortcomings of mitochondrial DNA for “barcoding” identification.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtlCru7jL&md5=f56101a94f8a21efa442294774f30254CAS |
Ryder Wilkie, K., Mertl, A., and Traniello, J. (2007). Biodiversity below ground: probing the subterranean ant fauna of Amazonia. Naturwissenschaften 94, 725–731.
| Biodiversity below ground: probing the subterranean ant fauna of Amazonia.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXos1Cjtrk%3D&md5=3cc74a554de7d75c4e533106a72e0ea0CAS |
Ryder Wilkie, K. T., Mertl, A. L., and Traniello, J. F. A. (2010). Species diversity and distribution patterns of the ants of Amazonian Ecuador. PLoS ONE 5, e13146.
| Species diversity and distribution patterns of the ants of Amazonian Ecuador.Crossref | GoogleScholarGoogle Scholar |
Schlick-Steiner, B. C., Steiner, F. M., Moder, K., Seifert, B., Sanetra, M., Dyreson, E., Stauffer, C., and Christian, E. (2006). A multidisciplinary approach reveals cryptic diversity in western Palearctic Tetramorium ants (Hymenoptera: Formicidae). Molecular Phylogenetics and Evolution 40, 259–273.
| A multidisciplinary approach reveals cryptic diversity in western Palearctic Tetramorium ants (Hymenoptera: Formicidae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XlvF2msr4%3D&md5=5186e8972a441133fb62ba09e4e2e5cbCAS |
Shoemaker, D. D., Ahrens, M. E., and Ross, K. G. (2006). Molecular phylogeny of fire ants of the Solenopsis saevissima species-group based on mtDNA sequences. Molecular Phylogenetics and Evolution 38, 200–215.
| Molecular phylogeny of fire ants of the Solenopsis saevissima species-group based on mtDNA sequences.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtlSiu7jN&md5=29cd239b0dc7f82a9ca00bf065148067CAS |
Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H., and Floors, P. (1994). Evolution, weighting, and phylogenetic utility of mitochondrial gene-sequences and a compilation of conserved polymerase chain-reaction primers. Annals of the Entomological Society of America 87, 651–701.
| 1:CAS:528:DyaK2MXis1Wiu7g%3D&md5=19fca4ef12a72211f41bc066c57d3b0dCAS |
Smith, M. R. (1943. [1942]). A new North American Solenopsis (Diplorhoptrum) (Hymenoptera: Formicidae). Proceedings of the Entomological Society of Washington 44, 209–211.
Smith, A. M., and Fisher, B. L. (2009). Invasions, DNA barcodes, and rapid biodiversity assessment using ants of Mauritius. Frontiers in Zoology 6, 31.
| Invasions, DNA barcodes, and rapid biodiversity assessment using ants of Mauritius.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhtFGrs7s%3D&md5=5a9d48744c36663e24988afd1494d71eCAS |
Smith, A. M., Fisher, B. L., and Hebert, P. D. N. (2005). DNA barcoding for effective biodiversity assessment of a hyperdiverse arthropod group: the ants of Madagascar. Philosophical Transactions of the Royal Society B: Biological Sciences 360, 1825–1834.
| DNA barcoding for effective biodiversity assessment of a hyperdiverse arthropod group: the ants of Madagascar.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtlSjsrjP&md5=9c43ff8e2fbf7cceae387b0951dfb02cCAS |
Smith, A. M., Eveleigh, E. S., McCann, K. S., Merilo, M. T., McCarthy, P. C., and Van Rooyen, K. I. (2011). Barcoding a quantified food web: crypsis, concepts, ecology and hypotheses. PLoS ONE 6, e14424.
| Barcoding a quantified food web: crypsis, concepts, ecology and hypotheses.Crossref | GoogleScholarGoogle Scholar |
Snelling, R. R. (2001). Two new species of thief ants (Solenopsis) from Puerto Rico (Hymenoptera: Formicidae). Sociobiology 37, 511–525.
Strutzenberger, P., Brehm, G., and Fiedler, K. (2011). DNA barcoding-based species delimitation increases species count of Eois (Geometridae) moths in a well-studied tropical mountain forest by up to 50%. Insect Science 18, 349–362.
| DNA barcoding-based species delimitation increases species count of Eois (Geometridae) moths in a well-studied tropical mountain forest by up to 50%.Crossref | GoogleScholarGoogle Scholar |
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28, 2731–2739.
| MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXht1eiu73K&md5=ff48f25899fc988f16ef1534cf14445fCAS |
Tänzler, R., Sagata, K., Surbakti, S., Balke, M., and Riedel, A. (2012). DNA barcoding for community ecology – how to tackle a hyperdiverse, mostly undescribed Melanesian fauna. PLoS ONE 7, e28832.
| DNA barcoding for community ecology – how to tackle a hyperdiverse, mostly undescribed Melanesian fauna.Crossref | GoogleScholarGoogle Scholar |
Taylor, H., and Harris, W. (2012). An emergent science on the brink of irrelevance: a review of the past 8 years of DNA barcoding. Molecular Ecology Resources 12, 377–388.
| An emergent science on the brink of irrelevance: a review of the past 8 years of DNA barcoding.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC38vjvVynug%3D%3D&md5=e2f38a9b9e6979e684f791bfb2a2f41eCAS |
Thompson, C. R. (1989). The thief ants, Solenopsis molesta group, of Florida (Hymenoptera: Formicidae). The Florida Entomologist 72, 268–283.
| The thief ants, Solenopsis molesta group, of Florida (Hymenoptera: Formicidae).Crossref | GoogleScholarGoogle Scholar |
Trager, J. C. (1991). A revision of the fire ants, Solenopsis geminata group (Hymenoptera: Formicidae: Myrmicinae). Journal of the New York Entomological Society 99, 141–198.
Tschinkel, W. R. (2006) ‘The Fire Ants.’ (Belknap Press of Harvard University Press.)
Tschinkel, W. R., Mikheyev, A. S., and Storz, S. R. (2003). Allometry of workers in the fire ant, Solenopsis invicta. Journal of Insect Science 3, 2.
Ward, P. S. (2000). Broad-scale patterns of diversity in leaf litter ant communities. In ‘Ants: Standard Methods for Measuring and Monitoring Biodiversity’. (Eds D. Agosti, J. D. Majer, L. E. Alonso, and T. R. Schultz.) pp. 99–121. (Smithsonian Institution Press: Washington, DC.)
Ward, P. S., and Downie, D. A. (2005). The ant subfamily Pseudomyrmecinae (Hymenoptera: Formicidae): phylogeny and evolution of big-eyed arboreal ants. Systematic Entomology 30, 310–335.
| The ant subfamily Pseudomyrmecinae (Hymenoptera: Formicidae): phylogeny and evolution of big-eyed arboreal ants.Crossref | GoogleScholarGoogle Scholar |
West-Wood, J. O. (1840). Observations on the genus Typhlopone, with descriptions of several exotic species of ants. Annals and Magazine of Natural History 6, 81–89.
| Observations on the genus Typhlopone, with descriptions of several exotic species of ants.Crossref | GoogleScholarGoogle Scholar |
Wetterer, J. K. (2011). Worldwide spread of the tropical fire ant, Solenopsis geminata (Hymenoptera: Formicidae). Myrmecological News 14, 21–35.
Wiemers, M., and Fiedler, K. (2007). Does the DNA barcoding gap exist? – a case study in blue butterflies (Lepidoptera: Lycaenidae). Frontiers in Zoology 4, 8.
| Does the DNA barcoding gap exist? – a case study in blue butterflies (Lepidoptera: Lycaenidae).Crossref | GoogleScholarGoogle Scholar |
Wild, A. L. (2009). Evolution of the Neotropical ant genus Linepithema. Systematic Entomology 34, 49–62.
| Evolution of the Neotropical ant genus Linepithema.Crossref | GoogleScholarGoogle Scholar |
Yassin, A., Markow, T. A., Narechania, A., O’Grady, P. M., and DeSalle, R. (2010). The genus Drosophila as a model for testing tree- and character-based methods of species identification using DNA barcoding. Molecular Phylogenetics and Evolution 57, 509–517.
| The genus Drosophila as a model for testing tree- and character-based methods of species identification using DNA barcoding.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtlOhtrjN&md5=a34d9262390fceba86cbae8f125611cbCAS |
