Adult and larval associations of the alpine stonefly genus Riekoperla McLellan (Plecoptera : Gripopterygidae) using mitochondrial DNA
J. H. Mynott A , J. M. Webb A B C and P. J. Suter AA Taxonomy Research Information Network, Department of Environmental Management and Ecology, La Trobe University, Wodonga, Vic. 3690, Australia.
B Present address: Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
C Corresponding author. Email: jmw975@yahoo.com
Invertebrate Systematics 25(1) 11-21 https://doi.org/10.1071/IS10025
Submitted: 26 August 2010 Accepted: 15 February 2011 Published: 14 July 2011
Abstract
The current taxonomic understanding of the genus Riekoperla McLellan, 1971 (Gripopterygidae) is poor, with 15 of the 28 species and subspecies having unknown or uncertain larval associations. Sequences of a 657 bp fragment from the mitochondrial gene cytochrome c oxidase subunit 1 (CO1) were obtained from 122 specimens of 13 species collected throughout the alpine areas of New South Wales and Victoria, Australia. Of these, sequence data associated adults and larvae for the following 10 species: R. alpina McLellan, 1971, R. cf. intermedia, R. compressa Theischinger, 1985, R. hynesorum Theischinger, 1985, R. karki McLellan, 1971, R. montana Theischinger, 1985, R. reticulata (Kimmins, 1951), R. rugosa (Kimmins, 1951), R. trapeza Theischinger, 1985, and R. tuberculata McLellan, 1971. Adults of R. intermedia Theischinger, 1985, R. triloba triloba McLellan, 1971 and R. williamsi McLellan, 1971 were sequenced but no larvae were associated with them. The 13 species were reciprocally monophyletic and had minimum interspecific sequence divergences ranging from 7.2–19.5%, higher than the maximum intraspecific sequence divergences (0.6–5.8%). The combination of morphology and molecular data enabled rapid life stage association for alpine Riekoperla species and this method should be used more frequently for other environmentally significant aquatic insects.
Additional keywords: Australia, association, cytochrome oxidase 1, molecular phylogenetics.
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