Cryptic diversity in Engaeus Erichson, Geocharax Clark and Gramastacus Riek (Decapoda : Parastacidae) revealed by mitochondrial 16S rDNA sequences
Mark B. Schultz A , Sarah A. Smith A , Alastair M. M. Richardson B , Pierre Horwitz C , Keith A. Crandall D and Christopher M. Austin E FA Arafura Timor Research Facility, School of Science and Primary Industries, Charles Darwin University, PO Box 41775, Casuarina, Northern Territory 0811, Australia.
B School of Zoology, University of Tasmania, Private Bag 5, Hobart, Tasmania 7001, Australia.
C School of Natural Sciences, Edith Cowan University, 100 Joondalup Drive, Joondalup, Perth, Western Australia 6027, Australia.
D Department of Integrative Biology, 675 Widstoe Building, Brigham Young University, Provo, UT 84602-5255, USA.
E School of Science and Primary Industries, Charles Darwin University, Darwin, Northern Territory 0909, Australia.
F Corresponding author. Email: chris.austin@cdu.edu.au
Invertebrate Systematics 21(6) 569-587 https://doi.org/10.1071/IS07019
Submitted: 12 May 2007 Accepted: 23 October 2007 Published: 18 December 2007
Abstract
Nucleotide sequence data from the mitochondrial 16S rDNA region were utilised to investigate phylogenetic relationships and species boundaries among Australian freshwater crayfish belonging to the genera Engaeus Erichson, 1846, Geocharax Clark, 1936 and Gramastacus Riek, 1972. Geocharax and Gramastacus were found to be monophyletic genera but one species currently assigned to Engaeus may belong to another genus. Relationships between the three existing genera were not resolved. Analysis of species boundaries within Geocharax suggests that there are an additional two species in this genus, and our analysis of Gramastacus indicates that undescribed populations from central New South Wales may comprise a second species. The data provide at least one instance of a taxon crossing the Great Dividing Range and provide confirmation of previously proposed hypotheses seeking to explain trans-Bass Strait distributions of species.
Additional keywords: molecular taxonomy, parastacid, phylogenetics, South Australia, south-eastern Australia, Tasmania, Tenuibranchiurus, Victoria.
Acknowledgements
This work was supported by ARC Discovery grant number DP0557840 to A.M.M.R., C.M.A., P.H. and K.A.C. For their help on various aspects of molecular and statistical genetics, we thank Dr Binh Thanh Thai, Dr Christopher P. Burridge, Dr Dianne Rowe, Dr Nicholas P. Murphy, Dr Adam D. Miller, Claire F. McClusky, Sally-Anne Williamson and Dr Todd Castoe. For their generation of some sequence data, we thank Dr Adam D. Miller, Assoc. Prof. Marina Paolucci and Dr Hemali Munasinghe. For access to museum specimens, we thank Dr Gary Poore and Dr Joanne Taylor (Museum Victoria), Dr Peter Davie (Queensland Museum), Dr George Wilson, Dr Stephen Keable and Dr Shane Ahyong (Australian Museum). For provision of samples, we thank Céleste Jacq, Claire McClusky, Dr Lachlan Farrington, Steve Wickson, Dr Belinda Robson, Kerrylyn Johnston, Simon McBeth, Dr Binh Thanh Thai, Tan Thi Nguyen, Hung Quang Le, Robert McCormack, Dr Adam Pope, Jarred Obst and David Nichol (GHCMA), Darren Welsh, George and Tony Beck (Lynwood Farm, South Australia) and Kãrlis Broders. Some field sampling was carried out under the Department of Sustainability and Environment Victoria Permit Number 10003070 (Dr Sue Hadden). For help with GIS and provision of equipment, we thank Neil Smit, Jai Sleeman, Dr Guy Boggs and Dr Daniel Ierodiaconou. For their assistance at the Arafura Timor Research Facility, we thank Stuart Fitch, Sabine L. M. Pircher, Bioscience North Australia and Charles Darwin University. We are grateful for the comments offered by three anonymous reviewers, which have helped us to improve this publication.
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