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RESEARCH ARTICLE
Contents Vol 19(3)

Phylogeny of the Australian freshwater crayfish Cherax destructor-complex (Decapoda : Parastacidae) inferred from four mitochondrial gene regions

Thuy T. T. Nguyen A B C and Christopher M. Austin A
+ Author Affiliations
- Author Affiliations

A School of Ecology and Environment, Deakin University, Warrnambool, Victoria 3280, Australia.

B Present address: Network of Aquaculture Centres in Asia-Pacific, PO Box 1040, Kasetsart Post Office, Bangkok 10903, Thailand.

C Corresponding author. Email: thuy.nguyen@enaca.org

Invertebrate Systematics 19(3) 209-216 https://doi.org/10.1071/IS04021
Submitted: 16 August 2004  Accepted: 11 April 2005   Published: 15 July 2005

Abstract

The phylogenetic relationships among 32 individuals of Australian freshwater crayfish belonging to the Cherax destructor-complex were investigated using a dataset comprising sequences from four mitochondrial gene regions: the large subunit rRNA (16S rRNA), cytochrome oxidase I (COI), adenosine triphosphatase 6 (ATPase 6), and cytochrome oxidase III (COIII). A total of 1602 bp was obtained, and a combined analysis of the data produced a tree with strong support (bootstrap values 94–100%) for three divergent lineages, verifying the phylogenetic hypotheses of relationships within the C. destructor species-complex suggested in previous studies. Overall, sequences from the 16S rRNA gene showed the least variation compared to those generated from protein coding genes, which presented considerably greater levels of divergence. The level of divergence within C. destructor was found to be greater than that observed in other species of freshwater crayfish, but interspecific variation among species examined in the present study was similar to that reported previously.


Acknowledgments

This research was supported by AusAID via an Australian Development Scholarship awarded to TTTN. Additional financial assistance was provided by a Deakin University internal grant to CMA and the Australian Pacific Science Foundation. Many people generously provided assistance in sampling including Dr Dean Jerry, Dr Lachlan Farrington, Stephen Ryan, Mike Truong, Dr Nick Murphy, Bernadette Bostock, and the Ryan family. Adam Miller helped by providing primers for sequencing. We greatly appreciated the advice of Dr Christopher Burridge in relation to data analysis and for reviewing the manuscript. TTTN is grateful to Professor Sena De Silva for his continuing support. Comments from the two anonymous reviewers are much appreciated.


References


Ahern M. D., Morris S. (1998) Accumulation of lead and its effects on Na+ balance in the freshwater crayfish Cherax destructor. The Journal of Experimental Zoology 281, 270–279.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Austin C. M. (1996) An electrophoretic and morphological taxonomic study of the freshwater crayfish genus Cherax (Decapoda: Parastacidae) in northern and eastern Australia. Australian Journal of Zoology 44, 259–296.
| Crossref |
open url image1

Austin C. M., Knott B. (1996) Systematics of the freshwater crayfish genus Cherax Erichson (Decapoda: Parastacidae) in south-western Australia: electrophoretic, morphological and habitat variation. Australian Journal of Zoology 44, 223–258.
| Crossref |
open url image1

Austin C. M., Nguyen T. T. T., Meewan M., Jerry D. R. (2003) The taxonomy and evolution of the Cherax destructor complex (Decapoda: Parastacidae) re-examined using mitochondrial 16S sequences. Australian Journal of Zoology 51, 99–110.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Basil J., Sandeman D. (2000) Crayfish (Cherax destructor) use tactile cues to detect and learn topographical changes in their environment. Ethology 106, 247–259.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Baum D. (1992) Phylogenetic species concepts. Trends in Ecology & Evolution 7, 1–2.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Campbell N. J. H., Geddes M. C., Adams M. (1994) Genetic variation in yabbies, Cherax destructor and C. albidus (Crustacea: Decapoda: Parastacidae), indicates the presence of a single, highly sub-structured species. Australian Journal of Zoology 42, 745–760.
| Crossref |
open url image1

Clark E. (1936) The freshwater and land crayfishes of Australia. Memoirs of the National Museum of Victoria 10, 5–56. open url image1

Clark E. (1941) New species of Australian freshwater crayfish and land crayfishes (family Parastacidae). Memoirs of the National Museum of Victoria 12, 31–40. open url image1

Cracraft J. (1989). Speciation and its ontology: the emperical consequences of alternative species concepts for understanding patterns and processes of differentiation. In ‘Speciation and its Consequences’. (Eds D. Otte and J. A. Endler.) pp. 28–37. (Sinauer: Sunderland, MA, USA.)

Crandall K. A. (2002) Crayfish as model organism.  13, 3–10. open url image1

de Queiroz K., Donoghue M. J. (1988) Phylogenetic systematics and the species problem. Cladistics 4, 317–338. open url image1

Dooley P. C., Crouch P. J., West J. M. (2002) Free amino acids in claw muscle and haemolymph from Australian freshwater crayfish at different stages of the moult cycle. Comparative Biochemistry and Physiology (A). Molecular and Integrative Physiology 131, 625–627.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Farris J., Källersjö M., Kluge A. G., Bult C. (1994) Testing significance of incongruence. Cladistics 10, 315–319.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Felsenstein J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution; International Journal of Organic Evolution 39, 783–791. open url image1

Fetzner J. W. J., and Crandall K. A. (2001). Genetic variation. In ‘Biology and Freshwater Crayfish’. (Ed. D. M. Holdich.) pp. 291–326. (Blackwell Science: Oxford, UK.)

Gilbert D. G. (1999). ‘SeqPup: A Biosequence Editor.’ (Biology Department, Indiana University: Bloomington, IN, USA.)

Hillis D. M., Huelsenbeck J. P. (1992) Signal, noise, and reliability in molecular phylogenetic analyses. The Journal of Heredity 83, 189–195.
| PubMed |
open url image1

Munasinghe D. H. N., Murphy N. P., Austin C. M. (2003) Utility of mitochondrial DNA sequences from four gene regions for systematic studies of Australian freshwater crayfish of the genus Cherax (Decapoda: Parastacidae). Journal of Crustacean Biology 23, 402–417. open url image1

Nguyen T. T. T. (2003). Molecular genetic studies of the yabby, Cherax destructor Clark. Ph.D. Thesis, School of Ecology and Environment, Deakin University, Warrnambool, Australia.

Nguyen T. T. T., Meewan M., Ryan S., Austin C. M. (2002a) Genetic diversity and translocation in the marron, Cherax tenuimanus (Smith): implications for management and conservation. Fisheries Management and Ecology 9, 163–173.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Nguyen T. T. T., Murphy N. P., Austin C. M. (2002b) Amplification of multiple copies of mitochondrial cytochrome b gene fragments in the Australian freshwater crayfish, Cherax destructor Clark (Parastacidae: Decapoda). Animal Genetics 33, 304–308.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Nguyen T. T. T., Austin C. M., Meewan M., Shultz M. B., Jerry D. R. (2004) Phylogeography of the freshwater crayfish Cherax destructor Clark (Parastacidae) in inland Australia: Historical fragmentation and recent range expansion. Biological Journal of the Linnean Society 83, 539–550.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Nguyen T. T. T., Burridge C. P., Austin C. M. (2005) Population genetic studies on the Australian freshwater crayfish, Cherax destructor (Crustacea: Parastacidae) using allozyme and RAPD markers. Aquatic Living Resources 18, 55–64.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Nixon K. C., Wheeler P. A. (1990) An amplification of the phylogenetic species concept. Cladistics 6, 211–223. open url image1

Palumbi S. R., Martin A., Romano S., McMillan W. O., Stice L., and Grabowski G. (1991). ‘The Simple Fools Guide to PCR. Version 2.’ (Department of Zoology, University of Hawaii: Honolulu, HI, USA.)

Posada D., Crandall K. A. (1998) ModelTest: testing the model of DNA substitution. Bioinformatics 14, 817–818.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Rice W. R. (1989) Analysing tables of statistical tests. Evolution; International Journal of Organic Evolution 43, 223–225. open url image1

Riek E. F. (1969) The Australian freshwater crayfish (Crustacea: Decapoda: Parastacidae), with description of new species. Australian Journal of Zoology 17, 855–918.
| Crossref |
open url image1

Scholtz G., Dohle W. (1996) Cell lineage and cell fate in crustacean embryo – a comparative approach. The International Journal of Developmental Biology 40, 211–220.
| PubMed |
open url image1

Shaw K. L. (1998). Species and the diversity of natural groups. In ‘Endless Forms: Species and Speciation’. (Eds D. J. Howard and S. H. Berlocher.) pp. 44–56. (Oxford University Press: Oxford, UK.)

Swofford D. L. (2001). ‘PAUP*: Phylogenetic Analysis Using Parsimony (* And Other Methods). Version 4b10.’ (Sinauer: Sunderland, MA, USA.)

Thompson J. D., Gibson T. J., Plewniak E., Jeanmougin E., Higgin D. G. (1997) The ClustalX windows interface: flexible strategies for multiple sequence aligment aided by quality analysis tools. Nucleic Acids Research 25, 4876–4882.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Versteegen M., Lawler S. H. (1997) Population genetics of the Murray River crayfish Eustacus armatus. Freshwater Crayfish 11, 146–157. open url image1

Walsh K. A., Macmillan D. L., Bourke D. W. (1994) The effect of ethanol on the dynamic response of a mechanoreceptor neuron. Research Communications in Substance Abuse 15, 9–20. open url image1

Whitington P. M., Leach D., Sandeman R. (1993) Evolutionary change in neural development within the arthropods – axonogenesis in the embryos of two crustaceans. Development 118, 449–461.
| PubMed |
open url image1