Transcriptional profiling of chickpea genes differentially regulated by salicylic acid, methyl jasmonate and aminocyclopropane carboxylic acid to reveal pathways of defence-related gene regulation
Tristan E. Coram A B C and Edwin C. K. Pang AA RMIT University, School of Applied Sciences, Biotechnology and Environmental Biology, Building 223, Level 1, Plenty Road, Bundoora, Vic. 3083, Australia.
B Present address: United States Department of Agriculture, Agricultural Service, 209 Johnson Hall, Washington State University, Pullman, WA 99163, USA.
C Corresponding author. Email: tcoram@mail.wsu.edu
Functional Plant Biology 34(1) 52-64 https://doi.org/10.1071/FP06250
Submitted: 9 October 2006 Accepted: 8 December 2006 Published: 19 January 2007
Abstract
Using microarray technology and a set of chickpea (Cicer arietinum L.) unigenes and grasspea (Lathyrus sativus L.) expressed sequence tags, chickpea responses to treatments with the defence signalling compounds salicylic acid (SA), methyl jasmonate (MeJA) and aminocyclopropane carboxylic acid (ACC) were studied in three chickpea genotypes with ranging levels of resistance to ascochyta blight [Ascochyta rabiei (Pass.) L.]. The experimental system minimised environmental effects and was conducted in reference design, where samples from untreated controls acted as references against post-treatment samples. Microarray observations were also validated by quantitative reverse transcription–polymerase chain reaction. The time-course expression patterns of 715 experimental microarray features resulted in differential expression of 425 transcripts. The A. rabiei resistant chickpea genotypes showed a more substantial range of defence-related gene induction by all treatments, indicating that they may possess stronger abilities to resist pathogens. Further, the involvement of SA, MeJA and ACC signalling was identified for the regulation of some important A. rabiei responsive transcripts, as well as cross-talk between these pathways. In the current study we also found evidence to suggest the involvement of A. rabiei-specific signalling mechanisms for the induction of several transcripts that were previously implicated in A. rabiei resistance. This study characterised the regulatory mechanisms of many chickpea transcripts that may be important in defence against various pathogens, as well as other cellular functions. These results provide novel insights to the molecular control of chickpea cellular processes, which may assist the understanding of chickpea defence mechanisms and allow enhanced development of disease resistant cultivars.
Additional keywords: ascochyta blight, microarray.
Acknowledgements
The authors acknowledge the Grains Research and Development Corporation (GRDC) for their generous granting of a research scholarship. We thank Dr Chris Pittock and Kristy Hobson (Department of Primary Industry, Horsham) for the supply of seeds, and Nitin Mantri (RMIT University) for assistance in microarray construction. We also thank Dr Beata Skiba (RMIT University) for the supply of grasspea clones.
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