Early accumulation of non-enzymatically synthesised oxylipins in Arabidopsis thaliana after infection with Pseudomonas syringae
Christoph Grun A , Susanne Berger A , Daniel Matthes A and Martin J. Mueller A BA Julius-von-Sachs-Institute for Biosciences, Pharmaceutical Biology, University of Wuerzburg, Julius-von-Sachs-Platz 2, D-97082 Würzburg, Germany.
B Corresponding author. Email: martin.mueller@biozentrum.uni-wuerzburg.de
Functional Plant Biology 34(1) 65-71 https://doi.org/10.1071/FP06205
Submitted: 23 August 2006 Accepted: 3 November 2006 Published: 19 January 2007
Abstract
The formation of non-enzymatic oxylipins is catalysed by reactive oxygen species. Reactive oxygen species are produced in response to pathogen attack. In this study, the accumulation of non-enzymatically formed hydroxy fatty acids and F1-phytoprostanes in leaves of Arabidopsis thaliana (L.) Heyhn upon infection with Pseudomonas syringae was investigated and compared with the accumulation of the enzymatically formed oxylipins jasmonic acid and 12-oxo-phytodienoic acid. Levels of all oxylipins increased after infection with a virulent and with an avirulent strain of P. syringae. Inoculation of the avirulent strain resulted in a biphasic accumulation with a first maximum around 5 h which was missing after inoculation of the virulent strain. Levels of free and esterified hydroxy fatty acids and F1-phytoprostanes increased after pathogen treatment; however, esterified compounds were 30 times more abundant than free oxylipins. The increase of the free compounds occurred later than the increase of the esterified compounds suggesting that non-enzymatic lipid oxidation occurs predominantly in membranes from which oxidised lipids can be released.
Additional keywords: hydroxy fatty acids, phytoprostanes.
Acknowledgements
We are grateful to M. Krischke for helpful discussion. This work was supported by the SFB 567.
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