Evaluating non-photochemical quenching (NPQ) kinetics and photosynthetic efficiency in cassava (Manihot esculenta) subjected to variable high light conditions
Raji Sadasivan Nair A , Saravanan Raju A * , Sanket Jijabrao More
B , Jos Thomas Puthur C , Jayanti Makasana D and Velumani Ravi A
A
B
C
D
Abstract
Light intensity is a critical environmental factor influencing plant growth and development. To survive high light conditions, plants have evolved various protective mechanisms, including non-photochemical quenching (NPQ). However, NPQ can limit effective photosynthetic yield when transitioning to low light conditions. This phenomenon is underexplored in cassava (Manihot esculenta), a starchy storage root crop known for its high biological efficiency and climate resilience. To address this knowledge gap, we assessed the photoprotective abilities and growth responses of six cassava varieties under natural environmental light conditions (control) and intermittent high light (IHL) conditions by adding 900 μmol m−2 s−1 using full-spectrum LED lights, on top of the natural ambient daylight. Our results demonstrated a significant impact of light treatment on aboveground biomass, total crop biomass, chlorophyll a and b content, photosynthetic rate, and NPQ values during transitions from low to high light and vice versa. Notably, cassava variety ‘Sree Suvarna’ exhibited the highest yield under both control and IHL conditions. These findings suggest that screening cassava varieties for their ability to postpone photoinhibition and recover quickly from photoinhibition may enhance photosynthetic performance. Such strategies have important implications for improving the efficiency and resilience of cassava crops, ultimately contributing to sustainable agricultural productivity.
Keywords: cassava, crop biomass, intermittent high light, light stress, NPQ induction rate, NPQ relaxation rate, photoinhibition, photosynthetic rate.
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