Cl– uptake, transport and accumulation in grapevine rootstocks of differing capacity for Cl–-exclusion
Joanna M. Tregeagle A B D E , Judy M. Tisdall B , Mark Tester C and Rob R. Walker AA CSIRO Plant Industry, PMB, Merbein, Vic. 3505, Australia.
B Department of Agricultural Sciences, La Trobe University, Vic. 3086, Australia.
C Australian Centre for Plant Functional Genomics and the University of Adelaide, Private Mail Bag 1, Glen Osmond, SA 5064, Australia.
D Present address: GHD Pty Ltd, 57 Orange Avenue, Mildura, Vic. 3500, Australia.
E Corresponding author. Email: rob.walker@csiro.au
Functional Plant Biology 37(7) 665-673 https://doi.org/10.1071/FP09300
Submitted: 16 December 2009 Accepted: 19 May 2010 Published: 2 July 2010
Abstract
Mechanisms of chloride (Cl–) exclusion in rootstocks of grapevine (Vitis spp.) were studied using a strong Cl–-excluding rootstock (140 Ruggeri) and a poor Cl–-excluding rootstock (K51–40). Xylem Cl– concentration in potted whole grapevines of 140 Ruggeri treated with 50 mM Cl– was 6.8-fold lower than that in K51–40. Five-fold lower total shoot Cl– in salt-treated 140 Ruggeri grapevines relative to that in K51–40 after 50 days, when shoots were of similar biomass, was unrelated to water use. Unidirectional influx of 36Cl– into excised roots (up to 30 min), and uptake of 36Cl– in roots of intact rooted-leaves (up to 180 min) in 10 mM Cl– was similar between the genotypes. However, net accumulation of Cl– by excised roots of K51–40 up to 180 min in 10 mM Cl– was significantly higher than that of 140 Ruggeri. Intact rooted-leaves of 140 Ruggeri in 10 mM Cl– accumulated higher Cl– concentrations in roots, and had a lower percentage of total plant 36Cl– accumulation in the shoot (petiole plus lamina) than those of K51–40. The greater Cl– exclusion capacity of 140 Ruggeri appears to be associated with restricted entry of Cl– to xylem and lower root to shoot Cl– transport.
Additional keywords: 140 Ruggeri, anion, influx, K51–40, roots, salinity.
Acknowledgements
This research was supported by the Commonwealth Cooperative Research Centre Program and was conducted through the former Cooperative Research Centre for Viticulture with support from Australia’s grape growers and winemakers through the Grape and Wine Research and Development Corporation, with matching funds from the Federal Government. The authors also thank Deepa Jha (Australian Centre for Plant Functional Genomics) for her technical assistance with flux experiments and Deidre Blackmore for assistance during manuscript preparation.
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