Sensitivity to high salinity in tetraploid citrus seedlings increases with water availability and correlates with expression of candidate genes
Wafa Mouhaya A B D , Thierry Allario A B D , Javier Brumos B , Fernando Andrés B , Yann Froelicher A , François Luro C , Manuel Talon B , Patrick Ollitrault A and Raphaël Morillon A B EA Centre de Cooperation Internationale en Recherche Agronomique pour le Développement (CIRAD), UPR Amélioration Génétique des Espèces Multiplication Végétative, Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain.
B Centro de Genomica, Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia, Spain.
C Unité Génétique et Ecophysiologie de la Qualité des Agrumes, Station Institut Nationale de la Recherche Agronomique de San Giuliano, 20230 San Giuliano, France.
D These authors contributed equally to the paper.
E Corresponding author. Email: raphael.morillon@cirad.fr
Functional Plant Biology 37(7) 674-685 https://doi.org/10.1071/FP10035
Submitted: 18 February 2010 Accepted: 23 May 2010 Published: 2 July 2010
Abstract
We investigated tolerance to high salinity in well-irrigated diploid and tetraploid citrus. Comparisons were made between two diploids (2×) of trifoliate orange (Poncirus trifoliata (L.) Raf.) and willow leaf mandarin (Citrus deliciosa Ten), their respective doubled diploids (4×) and the allotetraploid (FLHORAG1) obtained from the protoplast fusion of trifoliate orange and Willow leaf mandarin. Salinity stress was applied by progressively increasing the concentration of NaCl from 50 mM to 400 mM for 8 weeks. Two-year-old plants were watered daily. Maximum quantum yield of PSII, and leaf and root chloride and sodium content were monitored. We previously reported that under moderate saline stress, citrus 4× genotypes were more tolerant that the 2×, but under these experimental conditions, 4× seedlings were certainly more sensitive to salt stress than 2×, as they accumulated more toxic ions and were more affected than 2×. Chloride accumulation in 4× leaves was greater and the maximum quantum yield of PSII was more reduced in 4× than in 2×. The expression of several candidate genes involved in signal transduction, sodium and chloride transport, osmotic adjustment, regulation of the stomata opening and detoxification processes were also investigated by quantitative real-time reverse transcription-PCR. A high correlation was observed between phenotype of sensitivity to stress and gene expression changes.
Additional keywords: polyploidy, rootstocks, salt stress.
Acknowledgements
We thank C. Jacquemond, F. Curk (Unité GEQA, INRA, San Giuliano, France) for providing 2× seedlings. The authors thank the anonymous reviewers for their comments that helped to improve the manuscript. Funding was provided by Comité Mixte Inter Universitaire Franco-Marocain’ (Program Volubilis, MA/05/1 37) for WM and European INCO project (FP6 – 2003 - INCO – DEV – 2 n° 015453) for TA. Interreg IIIA program, Ministerio de Educacion y Ciencia, AGL 2007–65437-C04–01/AGR provided funding.
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