RNAi mediated silencing of dehydrin gene WZY2 confers osmotic stress intolerance in transgenic wheat
Zhengyang Yu
A , Xin Wang A , Xiaoqian Mu A and Linsheng Zhang A B
+ Author Affiliations
- Author Affiliations
A College of Life Science, Northwest A&F University, Yangling, Shaanxi, China.
B Corresponding author. Email: linszhang@nwsuaf.edu.cn
Functional Plant Biology 46(10) 877-884 https://doi.org/10.1071/FP19068
Submitted: 7 March 2019 Accepted: 18 May 2019 Published: 14 June 2019
Abstract
Dehydrins are involved in the prevention of osmotic damage in plants. Many studies have shown that overexpression of dehydrin genes can enhance the osmotic stress tolerance in transgenic plants. Our previous studies showed a YnSKn-type dehydrin gene WZY2 could be induced by polyethylene glycol (PEG), cold, indole-3-acetic acid (IAA), salicylic acid (SA) and abscisic acid (ABA). In the present study, we examined the phenotype and physiological indices in a dehydrin gene WZY2 RNA interference (RNAi) lines in wheat. Real-time PCR indicated a depressed WZY2 gene expression in transformed wheat. Furthermore, transgenic wheat showed lower relative water content, oxidative-related enzyme activities and higher malondialdehyde (MDA) content than wild-type bread wheat (Zhengyin No.1) under osmotic stress. Overexpression of the WZY2 in Arabidopsis thaliana (L.) Heynh. revealed a significant increase in tolerance to drought stress. Further studies also showed that WZY2 could participate in ABA-induced stomatal closure. These results demonstrated a key function of WZY2 in plant response to osmotic stress.
Additional keywords: dehydrins, LEA proteins, osmotic stress, oxidative stress, wheat.
References
Bao F, Du D, An Y, Yang W, Wang J, Cheng T, Zhang Q (2017) Overexpression of Prunus mume dehydrin genes in tobacco enhances tolerance to cold and drought. Frontiers in Plant Science 8, 151| Overexpression of Prunus mume dehydrin genes in tobacco enhances tolerance to cold and drought.Crossref | GoogleScholarGoogle Scholar | 28224001PubMed |
Chan Z, Shi H (2015) Improved abiotic stress tolerance of bermudagrass by exogenous small molecules. Plant Signaling & Behavior 10, e991577
| Improved abiotic stress tolerance of bermudagrass by exogenous small molecules.Crossref | GoogleScholarGoogle Scholar |
Chen RG, Jing H, Guo WL, Wang SB, Ma F, Pan BG, Gong ZH (2015) Silencing of dehydrin CaDHN1 diminishes tolerance to multiple abiotic stresses in Capsicum annuum L. Plant Cell Reports 34, 2189–2200.
| Silencing of dehydrin CaDHN1 diminishes tolerance to multiple abiotic stresses in Capsicum annuum L.Crossref | GoogleScholarGoogle Scholar | 26408144PubMed |
Close TJ (1997) Dehydrins: a commonality in the response of plants to dehydration and low temperature. Physiologia Plantarum 100, 291–296.
| Dehydrins: a commonality in the response of plants to dehydration and low temperature.Crossref | GoogleScholarGoogle Scholar |
Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. The Plant Journal 16, 735–743.
| Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.Crossref | GoogleScholarGoogle Scholar | 10069079PubMed |
Guo X, Zhang L, Zhu J, Liu H, Wang A (2017) Cloning and characterization of SiDHN, a novel dehydrin gene from Saussurea involucrata Kar. et Kir. that enhances cold and drought tolerance in tobacco. Plant Science 256, 160–169.
| Cloning and characterization of SiDHN, a novel dehydrin gene from Saussurea involucrata Kar. et Kir. that enhances cold and drought tolerance in tobacco.Crossref | GoogleScholarGoogle Scholar | 28167030PubMed |
Hara M, Terashima S, Fukaya T, Kuboi T (2003) Enhancement of cold tolerance and inhibition of lipid peroxidation by citrus dehydrin in transgenic tobacco. Planta 217, 290–298.
Hashimoto M, Negi J, Young J, Israelsson M, Schroeder JI, Iba K (2006) Arabidopsis HT1 kinase controls stomatal movements in response to CO2. Nature Cell Biology 8, 391–397.
| Arabidopsis HT1 kinase controls stomatal movements in response to CO2.Crossref | GoogleScholarGoogle Scholar | 16518390PubMed |
Jiang X, Wang Y (2004) β-Elimination coupled with tandem mass spectrometry for the identification of in vivo and in vitro phosphorylation sites in maize dehydrin DHN1 protein. Biochemistry 43, 15567–15576.
| β-Elimination coupled with tandem mass spectrometry for the identification of in vivo and in vitro phosphorylation sites in maize dehydrin DHN1 protein.Crossref | GoogleScholarGoogle Scholar | 15581369PubMed |
Li Q, Zhang X, Lv Q, Zhu D, Qiu T, Xu Y, Bao F, He Y, Hu Y (2017) Physcomitrella patens dehydrins (PpDHNA and PpDHNC) confer salinity and drought tolerance to transgenic Arabidopsis plants. Frontiers in Plant Science 8, 1316
| Physcomitrella patens dehydrins (PpDHNA and PpDHNC) confer salinity and drought tolerance to transgenic Arabidopsis plants.Crossref | GoogleScholarGoogle Scholar | 28798765PubMed |
Mori IC, Murata Y, Yang Y, Munemasa S, Wang YF, Andreoli S, Tiriac H, Alonso JM, Harper JF, Ecker JR, Kwak JM, Schroeder JI (2006) CDPKs CPK6 and CPK3 function in ABA regulation of guard cell S-type anion- and Ca(2+)-permeable channels and stomatal closure. PLoS Biology 4, e327
| CDPKs CPK6 and CPK3 function in ABA regulation of guard cell S-type anion- and Ca(2+)-permeable channels and stomatal closure.Crossref | GoogleScholarGoogle Scholar | 17032064PubMed |
Mouillon JM, Eriksson SK, Harryson P (2008) Mimicking the plant cell interior under water stress by macromolecular crowding: disordered dehydrin proteins are highly resistant to structural collapse. Plant Physiology 148, 1925–1937.
| Mimicking the plant cell interior under water stress by macromolecular crowding: disordered dehydrin proteins are highly resistant to structural collapse.Crossref | GoogleScholarGoogle Scholar | 18849483PubMed |
Mustilli AC, Merlot S, Vavasseur A, Fenzi F, Giraudat J (2002) Arabidopsis OST1 protein kinase mediates the regulation of stomatal aperture by abscisic acid and acts upstream of reactive oxygen species production. The Plant Cell 14, 3089–3099.
| Arabidopsis OST1 protein kinase mediates the regulation of stomatal aperture by abscisic acid and acts upstream of reactive oxygen species production.Crossref | GoogleScholarGoogle Scholar | 12468729PubMed |
Negi J, Matsuda O, Nagasawa T, Oba Y, Takahashi H, Kawai-Yamada M, Uchimiya H, Hashimoto M, Iba K (2008) CO2 regulator SLAC1 and its homologues are essential for anion homeostasis in plant cells. Nature 452, 483–486.
| CO2 regulator SLAC1 and its homologues are essential for anion homeostasis in plant cells.Crossref | GoogleScholarGoogle Scholar | 18305482PubMed |
Pedrosa AM, Martins Cde P, Goncalves LP, Costa MG (2015) Late embryogenesis abundant (LEA) constitutes a large and diverse family of proteins involved in development and abiotic stress responses in sweet orange (Citrus sinensis L. Osb.). PLoS One 10, e0145785
| Late embryogenesis abundant (LEA) constitutes a large and diverse family of proteins involved in development and abiotic stress responses in sweet orange (Citrus sinensis L. Osb.).Crossref | GoogleScholarGoogle Scholar | 26700652PubMed |
Saibi W, Feki K, Ben Mahmoud R, Brini F (2015) Durum wheat dehydrin (DHN-5) confers salinity tolerance to transgenic Arabidopsis plants through the regulation of proline metabolism and ROS scavenging system. Planta 242, 1187–1194.
| Durum wheat dehydrin (DHN-5) confers salinity tolerance to transgenic Arabidopsis plants through the regulation of proline metabolism and ROS scavenging system.Crossref | GoogleScholarGoogle Scholar | 26105651PubMed |
Sierla M, Horak H, Overmyer K, Waszczak C, Yarmolinsky D, Maierhofer T, Vainonen JP, Salojarvi J, Denessiouk K, Laanemets K, Toldsepp K, Vahisalu T, Gauthier A, Puukko T, Paulin L, Auvinen P, Geiger D, Hedrich R, Kollist H, Kangasjarvi J (2018) The receptor-like pseudokinase GHR1 is required for stomatal closure. The Plant Cell 30, 2813–2837.
Sofronova VE, Maximov TC, Korotaeva NE, Suvorova GG, Oskorbina MV, Borovskii GB (2012) Accumulation of heat shock proteins and dehydrins in the needles of scotch pine at the early stage of the PSII photoinhibition during the autumn adaptation of plants to winter conditions. Doklady Biological Sciences 443, 113–116.
| Accumulation of heat shock proteins and dehydrins in the needles of scotch pine at the early stage of the PSII photoinhibition during the autumn adaptation of plants to winter conditions.Crossref | GoogleScholarGoogle Scholar | 22562683PubMed |
Su J, Lu X, Zhu W, Zhang L (2012) Construction of RNAi expression vector of WZY2 gene from wheat and its genetic transformation. Xibei Nong-Lin Keji Daxue Xuebao. Ziran Kexue Ban 40, 66–72.
Umezawa T, Sugiyama N, Mizoguchi M, Hayashi S, Myouga F, Yamaguchi-Shinozaki K, Ishihama Y, Hirayama T, Shinozaki K (2009) Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America 106, 17588–17593.
| Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis.Crossref | GoogleScholarGoogle Scholar | 19805022PubMed |
Xie C, Zhang R, Qu Y, Miao Z, Zhang Y, Shen X, Wang T, Dong J (2012) Overexpression of MtCAS31 enhances drought tolerance in transgenic Arabidopsis by reducing stomatal density. New Phytologist 195, 124–135.
| Overexpression of MtCAS31 enhances drought tolerance in transgenic Arabidopsis by reducing stomatal density.Crossref | GoogleScholarGoogle Scholar | 22510066PubMed |
Yang W, Zhang L, Lv H, Li H, Zhang Y, Xu Y, Yu J (2015) The K-segments of wheat dehydrin WZY2 are essential for its protective functions under temperature stress. Frontiers in Plant Science 6, 406
| The K-segments of wheat dehydrin WZY2 are essential for its protective functions under temperature stress.Crossref | GoogleScholarGoogle Scholar | 26124763PubMed |
You J, Chan Z (2015) ROS regulation during abiotic stress responses in crop plants. Frontiers in Plant Science 6, 1092
| ROS regulation during abiotic stress responses in crop plants.Crossref | GoogleScholarGoogle Scholar | 26697045PubMed |
Zhu W, Zhang L, Lv H, Zhang H, Zhang D, Wang X, Chen J (2014) The dehydrin wzy2 promoter from wheat defines its contribution to stress tolerance. Functional & Integrative Genomics 14, 111–125.
| The dehydrin wzy2 promoter from wheat defines its contribution to stress tolerance.Crossref | GoogleScholarGoogle Scholar |
