Overexpression of Triticum durum TdAnn12 gene confers stress tolerance through scavenging reactive oxygen species in transgenic tobacco
Rania Ben Saad A E , Marwa Harbaoui A , Walid Ben Romdhane A B , Nabil Zouari A , Khong N. Giang D , Anis Ben Hsouna A C and Faical Brini A
+ Author Affiliations
- Author Affiliations
A Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax (CBS)/University of Sfax, B.P. ‘1177’ 3018, Sfax, Tunisia.
B Plant Production Department, College of Food and Agricultural Sciences, King Saud University, PO Box 2460, 11451 Riyadh, Saudi Arabia.
C Department of Life Sciences, Faculty of Sciences of Gafsa, Zarroug 2112, Gafsa, Tunisia.
D International Joint Laboratory (LMI-RICE2), National Key Laboratory of Plant Cell Biotechnology, Agricultural Genetics Institute (AGI), Vietnam Academy of Agriculture Sciences (VAAS), Km2 Pham Van Dong Road, Co Nhue, Tu Liem District, Hanoi 10000, Vietnam.
E Corresponding author. Email: raniabensaad@gmail.com
Functional Plant Biology 46(10) 885-895 https://doi.org/10.1071/FP18316
Submitted: 30 November 2018 Accepted: 22 May 2019 Published: 14 June 2019
Abstract
Plant annexins are proteins with multiple functions and roles in plant development and responses to abiotic stresses. We report here the functional analysis of the TdAnn12 annexin protein isolated from Triticum durum Desf. We have previously shown that TdAnn12 expression is highly induced by different abiotic stresses. In the present study, to investigate the physiological and biochemical stress-induced responses, we overexpressed TdAnn12 in tobacco. We demonstrate that transgenic tobacco plants expressing TdAnn12 exhibited enhanced tolerance to salt, osmotic stress and H2O2 at the seedling stage. Under greenhouse conditions, these plants showed tolerance to drought and salt stresses. Moreover, scavenging reactive oxygen species (ROS), higher chlorophyll content, lower lipid peroxidation levels and increased antioxidant activities (peroxidase, catalase and superoxide dismutase) were observed. Finally, accumulation of TdAnn12 in tobacco positively affects the regulation of some stress-related genes (MnSOD, APX1, CAT1, P5CS, NHX1, SOS1 and DREB1A). TdAnn12 interacts directly or indirectly with stress-related genes that could stimulate an adaptive potential to gain tolerance which is not present in non-transgenic (NT) plants. Our results clearly show that overexpression of TdAnn12 in transgenic tobacco improves stress tolerance through the removal of ROS.
Additional keywords: durum abiotic stress tolerance, transgenic plants, wheat.
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