Acidification increases mercury uptake by a freshwater alga, Chlamydomonas reinhardtii
Séverine Le Faucheur A B , Yvan Tremblay A C , Claude Fortin A and Peter G. C. Campbell A D
+ Author Affiliations
- Author Affiliations
A Institut national de la Recherche scientifique, INRS Eau Terre et Environnement, 490 de la Couronne, Québec, QC, G1K 9A9, Canada.
B Present address: Institut Forel, Université de Genève, 10 route de Suisse, CH-1290 Versoix, Switzerland.
C Present address: Bureau d’audiences publiques sur l’environnement, 575 rue Saint-Amable, Québec, QC, G1R 6A6, Canada.
D Corresponding author. Email: peter.campbell@ete.inrs.ca
Environmental Chemistry 8(6) 612-622 https://doi.org/10.1071/EN11006
Submitted: 13 January 2011 Accepted: 25 August 2011 Published: 17 November 2011
Environmental context. Mercury is classified as a priority pollutant owing to the biomagnification of its methylated species along food chains and the consequent effects on top consumers. The pH of natural waters affects many of the biogeochemical processes that control mercury accumulation in aquatic organisms. Here, evidence is presented that pH affects mercury uptake by unicellular algae, primary producers in aquatic food chains, thereby providing a new example of the pervasive influence of pH on the mercury biogeochemical cycle.
Abstract. We have examined the influence of pH on HgII uptake (mainly in the form of the lipophilic complex HgCl2) by a green, unicellular alga, Chlamydomonas reinhardtii. Uptake of the dichloro complex increased by a factor of 1.6 to 2 when the pH was lowered from 6.5 to 5.5, an unexpected result given that the intracellular hydrolysis rate of fluorescein diacetate (FDA), used as a probe for the passive diffusion of lipophilic solutes through algal membranes, decreased in the studied alga under similar conditions. Several mechanisms were explored to explain the enhanced uptake at pH 5.5, including pH-induced changes in cell surface binding of Hg or in Hg loss rates from cells, but none of them gave completely satisfactory explanations. The present findings imply that inorganic HgII in aqueous solution behaves, in terms of uptake, neither as a lipophilic complex (the uptake of which would be expected to decrease with acidification because of algal membrane packing), nor as a cationic metal (the transport of which by facilitated transport would be expected to diminish with increasing proton concentration because of metal–proton competition at the transporter binding sites). Mercury uptake by algae seems rather to be stimulated by proton addition.
Additional keywords: cell membrane, lipophilic metal complex, pH, unicellular green algae, uptake rate.
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